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Chapter 5: Installation
Thermo Scientific Wellwash
®
4 Mk 2 User Manual Rev.2.3, Cat. no. 1507340
25
second intervals until the PRIME indicator is reached. The pressure
generation takes 10 seconds. The PRIME indicator continues flashing to
prompt the user to prime the system.
4.
Select the preprogrammed wash card for 1 wash and for the 12-way wash
head. Plug it into the slot on the right-hand side of the washer unit (Fig. 4.1,
item 13).
5.
Lift the wash head and slide the plate carrier onto the stainless steel
slideway so that the reservoir is under the wash head (Fig. 5.13).
6.
The instrument should now be primed. Press the PRIME button to initiate
the sequence. The wash head will lift and the pinch valve will open for one
second allowing wash fluid to flow into the priming reservoir. The wash
head will then lower itself to empty the prime reservoir. The PRIME
indicator will turn off when the priming is complete.
If no further key entry is made within ten (10) seconds of priming, the pump
will automatically switch OFF. The instrument will then enter its idle state,
waiting for instructions from the keypad and program card.
7.
Place a microplate onto the plate carrier. When using a 12-way wash head,
the well A1 of the microplate should be positioned in the top left-hand
corner. When using an 8-way wash head, the well A1 should be positioned
in the top right-hand corner. See Fig. 5.16.
Fig. 5.16 Placing the microplate with 12-way and 8-way wash head
8.
Select the row number of the last row to be washed on the keypad (e.g.,
when button 4 is pressed, rows 1 to 4 will be washed).
Note:
When the 12-way program card is installed, buttons 9 to 12 are
inoperative.
9.
Press the START button to set the washing cycle into operation. The plate
carrier will be drawn into the washer, filling and aspirating each row
according to the sequence selected on the program card. At the end of a
sequence, rows will be left substantially dry and the plate carrier will stop
with the priming reservoir under the wash head.
10.
The instrument is performing properly, if the wells are dry. If they are not,
check that both bottle caps are firmly closed
A1
A1
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