ThermoFisher Scientific E-PAGE Gels, Техническое руководство

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29
Using the iBlot
®
Device with the De-Bubbling Roller,
Continued
Assembling the
iBlot ® Device,
continued
11. Hold the iBlot ® E-PAGE ™ Tab and plastic tab on the iBlot ® Anode Stack,
Bottom together and pull the assembly (anode and cathode stacks, and gel)
together through the De-bubbling Roller towards the blotting surface, in
one smooth, uninterrupted movement until the assembly reaches the Gel
Barriers on the blotting surface (figure A). At the end of de-bubbling, all
layers are aligned to the right as shown below (figure B).
A B
12. Place the iBlot
®
Disposable Sponge on the inner side of the lid (between the
small protrusions on the lid that hold the sponge in its place) such that the
metal contact is to the top right.
The sponge absorbs any excess liquid generated during blotting and exerts
an even pressure on the stack surface.
Performing
Blotting
After assembling the iBlot
®
Gel Transfer Device, perform blotting within
15 minutes of assembling the stacks with the gel as described below.
1. Close the iBlot ® Lid and secure the latch. The red light is on indicating a
closed circuit. Ensure the correct program is selected (Program P3 at 20 volts
for 7–8 minutes).
2. Press the Start/Stop button to start the transfer. The red status light changes
to green. The transfer continues using the programmed parameters.
3. At the end of the transfer, current automatically shuts off and the iBlot ® Gel
Transfer Device signals the end of transfer with repeated beeping sounds,
and flashing red light and digital display.
Note: Previous versions of the iBlot ® Gel Transfer Device (firmware versions
prior to 2.7.9), signaled the end of transfer with repeated beeping sounds,
and flashing green light (instead of red light) and digital display.
4. Press and release the Start/Stop button to stop the beeping. The light turns
to a steady red light.
Contact