44
SimplyBlue
™
SafeStain,
Continued
E-PAGE
96
Gels
For routine staining of E-PAGE
96
Gels, use Protocol A. To obtain the clearest
background for imaging, use Protocol B, which includes a 12–24 hour washing
step to improve the background.
Protocol A
1.
After electrophoresis, remove the gel from the cassette (page 24) and place
the gel in a clean incubation tray.
2.
Rinse the gel 3 times for 5 minutes each with deionized water to remove SDS
and buffer salts.
3.
Stain the gel with sufficient SimplyBlue
SafeStain to cover the gel. Incubate
at room temperature for 1.5 hours with gentle shaking. Decant the stain.
4.
Wash the gel with deionized water for 3 hours with intermittent water
changes.
Protocol B
1.
After electrophoresis, remove the gel from the cassette (page 24) and place
the gel in a clean incubation tray.
2.
Fix the gel in 20% acetic acid for 30 minutes at room temperature. Decant the
solution.
3.
Stain the gel with sufficient SimplyBlue
SafeStain to cover the gel. Incubate
at room temperature for 30 minutes with gentle shaking. Decant the stain.
4.
Rinse the gel briefly with deionized water. Wash the gel in deionized water
for 12–24 hours at room temperature, changing the water at least once.
Содержание E-PAGE Gels
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