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Section 3 - Calibration
3.1 Calibration Mode
After the unit has stabilized, several different systems can
be calibrated. In Calibration mode, the air temperature, CO
2
and RH levels can be calibrated to reference instruments. To
access Calibration mode, press the Mode key until the Calibrate
indicator lights. Press the right and/or left arrow until the
appropriate parameter appears in the message center. See Chart
2-1 at the end of this section for more detail.
Calibration frequency is dependent on use, ambient condi-
tions and accuracy required. A good laboratory practice would
require at least an annual calibration check. On new installa-
tions, all parameters should be checked after the stabilization
period.
Prior to calibration, the user should be aware of the follow-
ing system functions. While the unit is in Calibration mode, all
system control functions are disabled so the unit remains stable.
Readout of the system being calibrated will appear on the mes-
sage center. If no keys are pressed for approximately five min-
utes while in Calibration mode, the system will reset to Run
mode so control functions are reactivated.
Before making any calibration or adjustments to
the unit, it is imperative that all reference instru-
ments be properly calibrated.
a. Calibrating the Temperature
Before calibration, allow the cabinet temperature to stabi-
lize. Place the calibrated instrument in the center of the cham-
ber. The instrument should be in the airflow, not against the
shelf.
Temperature Stabilization Periods
Startup
- Allow 12 hours for the temperature in the cabinet to
stabilize before proceeding.
Presently Operating
- Allow at least 2 hours after the display
reaches setpoint for temperature to stabilize before proceeding.
1. Press the Mode key until Calibrate indicator lights.
2. Press the right arrow until “TEMP CAL XX.XC”
appears in the message center.
3. Press up/down arrow to match display to calibrated
instrument.
4. Press Enter to store calibration.
5. Press the Mode key to return to Run or the right/left
arrow to go to next/previous parameter.
3 - 1
b. Calibrating the Infrared CO
2
System
All models of the Steri-Cult incubator have an infrared (IR)
CO
2
sensor. Infrared CO
2
sensors are not effected by chamber
atmosphere temperature or humidity.
CO
2
Sensor Stabilization Times
Start-Up
- Allow the CO
2
of the cabinet to stabilize at least 12
hours before proceeding.
Presently Operating
- Allow CO
2
to stabilize at least 2 hours
at setpoint before proceeding.
1. Measure the CO
2
concentration in the chamber through
the gas sample port with a Fyrite or other independent
instrument. Several readings should be taken to ensure
accuracy.
2. Press the Mode key until the Calibrate indicator lights.
3. Press the right arrow until “CO2 CAL XX.X%” appears
in the message center.
4. Press the up/down arrow to adjust the display to match
the independent instrument reading.
5. Press Enter to store the calibration.
6. Press the Mode key to return to Run mode.
c. Calibrating Relative Humidity
Place an accurate independent instrument in the center of
the chamber. Most RH measuring devices are least accurate
above 90%. It is recommended that when calibrating above
90%, the incubator should be adjusted to the lower edge of the
instrument accuracy range. For example; with the incubator set
at 95%, the independent instrument with accuracy of ±5% reads
94%. Calibrate the incubator display to 99%. (This prevents
condensation issues caused by actual RH levels above 95%.)
Relative Humidity Stabilization Times
Start-Up
- Allow 12 hours for the relative humidity and tem-
perature in the chamber to stabilize before proceeding.
Presently Operating
- Allow at least 2 hours after temperature
display reaches setpoint for relative humidity to stabilize before
proceeding.
1.
Press the Mode key until the Calibrate indicator lights.
2. Press the right arrow key until “RH CAL XXX%”
appears in the message center.
3. Press the up/down arrow to match the display to the
independent instrument.
4. Press Enter to store the calibration.
5. Press the Mode key to return to Run mode.
Steri-Cult Incubator _________________________________________________________________________Calibration
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