Test Procedures
Page 32 of 60
M1393R0
OQ/PQ Operating Instructions
4.7.1
Performing the UV Detector Linearity Test
Depending on the system configuration, the ‘
UV Detector linearity_NAN’
or ‘
UV Detector
linearity_CAP’
sequence is used to perform the detector linearity test. The detector
linearity is determined at 272 nm using caffeine standard samples with different
concentrations:
•
CAP configuration: 0.25, 0.5, 1.0, 2.0, 5.0, 10.0 and 20.0
µ
g/mL
•
NAN configuration: 1.0, 2.0, 5.0, 10.0 , 20.0, 40.0 and 60.0
µ
g/mL
Install the samples in their proper positions in the FAMOS autosampler rack (TABLE 3-5).
TABLE 2-1 presents the conditions for the linearity test for the two different system
configurations.
TABLE 4-1
Experimental Conditions – UV Detector Linearity
Parameter
Nano Configuration
Capillary Configuration
Detection wavelength
272 nm
272 nm
Run time
8 min
4 min
Flow rate
0.4
µ
L/min 4.0
µ
L/min
CRP 625
50
Injection loop
1.0
µ
L 1.0
µ
L
Injection type
Partial Loop Fill, 0.2 µL
Partial Loop Fill, 0.5 µL
To perform the UV Detector linearity test:
a)
Setup the UltiMate system and the FAMOS Microautosampler as discussed in Sections
3.2.2 A and 3.2.2 B.
b)
Start the flow delivery of the UltiMate Micropump from the CHROMELEON control panel.
c)
After equilibration, start the sequence ‘XQ_3_UV_Linearity_NAN’ (or ‘_CAP’,
respectively).
To evaluate the result of the ‘UV Detector linearity Test’:
a)
Use the quantization file ‘
caffeine.qnt’.
b)
Check the integration and correct if necessary.
c)
Verify that the correlation coefficient meets the acceptance criteria (TABLE 2-3).
