Process
Page 20 of 60
M1393R0
OQ/PQ Operating Instructions
3.2.2 B
FAMOS Microautosampler
To prepare the FAMOS Microautosampler for the OQ/PQ tests:
a)
Position the caffeine standards of the OQ/PQ kit in the FAMOS sample rack as
presented in TABLE 3-5.
TABLE 3-5
Sample Positions in the Autosampler Rack
Tray Position
Sample
A2
0.25
µ
g/ml Caffeine (CAP system only)
A3
0.5
µ
g/ml Caffeine
(CAP system only)
A4
1.0
µ
g/ml Caffeine
A5
2.5
µ
g/ml Caffeine
A6
5.0
µ
g/ml Caffeine
A7
10.0
µ
g/ml Caffeine
A8
20.0
µ
g/ml Caffeine
B1
40.0
µ
g/ml Caffeine (NAN system only)
B2
60.0
µ
g/ml Caffeine (NAN system only)
b)
Use mobile phase A as the wash solvent (TABLE 3-4).
c)
Degas the wash solvent. Helium sparging is strongly recommended.
d)
Check that there is no air in the syringe and run a wash cycle on the instrument.
3.2.2 C
Switchos Advanced Microcolumn Switching Unit
To prepare the Switchos Advanced Microcolumn Switching Unit for the OQ/PQ:
a)
Prepare the Switchos with the following mobile phases
Solvent A:
0.1 % TFA in water
Solvent
B:
0.1
% TFA in water
Solvent
C:
0.1 % TFA in water
Solvent
D:
0.1
% TFA in water.
b)
Connect a trap-column connecting tubing 30
µ
m ID x 15 cm (P/N 160182) to port 2 of
switching valve A.
c)
Start helium sparging to degas the solvents.
d)
Thoroughly purge all channels as described in the user’s manual of the instrument.
