Prepare samples using MicroAmp
™
tube strips with
attached caps
1.
Separate the blue tray and retainer by squeezing the release
catch as indicated in the graphic.
1
2
3
1
1
Release catch
2
MicroAmp
™
96-Well Retainer
3
MicroAmp
™
96-Well Tray
2.
Place the blue tray on the 96-well base.
3.
Load the tube strips on the tray.
4.
Pipette the reaction mixture into the tubes.
5.
Seal the tube strip using a MicroAmp
™
cap strip. See “Seal
tubes strips with cap strips“ on page 5 for instructions.
6.
Remove the blue tray containing the sealed tube strips from
the 96-well base and place the tray and sealed tube strips into
the instrument.
1
MicroAmp
™
8-Tube Strip with Attached Caps (0.2-mL)
2
MicroAmp
™
96-Well Tray
3
MicroAmp
™
Splash Free 96-Well Base
Seal tubes strips with cap strips
IMPORTANT!
Apply significant downward pressure on the
sealing tool in all steps to form a complete seal on top of the tubes.
1.
Align and place the cap strips on the tubes.
2.
Seal the cap strips using the rocking capping tool:
a.
Slip your fingers through the handle with the holes in
the tool facing down.
b.
Place the holes in the tool over the first eight caps in a
row.
c.
Rock the tool back and forth a few times to seal the
caps.
d.
Repeat for remaining caps in the row, then for all
remaining rows.
Prepare samples using MicroAmp
™
Reaction Tubes
1.
Set the black tray on a 96-well base.
2.
Place the reaction tubes in the black tray.
1
2
3
1
MicroAmp
™
Reaction Tube with Cap (0.2-mL)
2
MicroAmp
™
96-Well Tray for VeriFlex
™
Blocks
3
MicroAmp
™
Splash Free 96-Well Base
3.
Pipette the reaction mixture into the reaction tubes.
4.
Cap the tubes.
5.
Remove the black tray with sealed reaction tubes from the
96-well base and place the tray and sealed tubes into the
instrument.
SimpliAmp
™
Thermal Cycler Installation and Operation Quick Reference
5