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Troubleshooting Guide
Warning and error codes
52
Thermo Scientific PikoReal Real-Time PCR System User Manual
Thermo Fisher Scientific
When an error is detected, the current operation is aborted. After an
error, it is best to restart from the beginning after the problem has been
fixed. When an error occurs, the error code and the suggested actions
are presented. You can restore the data of the last run in event of
technical problems.
Press [
C
]
to clear the warning or error message.
Table 7–5.
Warnings
Code
Symbolic name
Description
Action text
W1
POWER FAILURE
This warning is set if the power was
lost during protocol execution for less
than 24 h and the protocol was
recovered.
CHECK LOGFILES
W8
BLOCK TEMP HIGH
This warning is set if the block
temperature is over 112°C.
W9
LID TEMP HIGH
This warning is set if the lid
temperature is over 115°C.
W10
SINK TEMP HIGH
This warning is set if the sink
temperature is over 75°C.
CHECK THAT THE
HEATSINK IS CLEAN
W27
PROTOCOL STOPPED
This warning is set if the protocol is
stopped before the end of the protocol.
W28
CAMERA FAILURE
This warning is set if the first camera
plate read command fails (only on RT).
W29
PROTOCOL PAUSED
This warning is set if the protocol is
paused during the protocol run.
CHECK LOGFILES
W30
CYCLES ADDED
This warning is set if extra cycles are
added to a protocol loop.
CHECK LOGFILES
W31
STEP SKIPPED
This warning is set if the protocol step
is skipped (NEXT).
CHECK LOGFILES
W32
LID TEMP OVERRIDDEN
This warning is set if the lid
temperature is overridden from the
service menu.
CHECK LOGFILES
Table 7–6.
Errors
Code
Symbolic name
Description
Action text
E1
POWER FAILURE
This error is set if the power was lost
during protocol execution for over 24 h.
CHECK LOGFILES
E3
BLOCK TEMP.
This error is set if the block
temperature is over 115°C.
CONTACT SUPPORT
E4
LID TEMP.
This error is set if the lid temperature
is over 125°C.
CONTACT SUPPORT
E5
SINK TEMP.
This error is set if the sink temperature
is over 85°C.
ENSURE THAT THE
AIRFLOW TO THE FAN
IS NOT OBSTRUCTED.
IF PROBLEM PERSIST
CONTACT SUPPORT
Continued
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