Thermo Scientific OWL HEP-1, Руководство по эксплуатации и техническому обслуживанию

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5-2 Semidry Electroblotter Thermo Scientific
Section 5
Technical Tips
1. Bjerrum, O.J. and Schafer-Nielsen, C. in: Dunn, J.J. (ed.)
Electrophoresis ‘86 VCH Weinheim 1986, pp. 315-327. These authors
compare results using different transfer buffers (Towbin buffer vs. the
three buffer system).
2. Khyse-Anderson, J 1984. Electroblotting of multiple gels. A simple
apparatus without buffer tank for rapid transfer of proteins from
polyacrylamide gels to nitrocellulose. J. Biochem. Biophys. Methods
10: 203-209. This paper describes the semidry blotter with a 3 buffer
system (Owl ER35) which is effective for transfer of proteins.
Protein Blotting
3. Castora, Frank 1, “Western Blotting of Proteins”, Clinical
Biotechnology 1: 43-49 (1989). This review article on Western
Blotting gives a good overview of factors such as transfer buffers, types
of membranes, and post-membrane stains. Although written for
standard tank blotting, much of this is applicable also to semidry
blotting.
4. Eckerskorn, Christoph and Lottspeich, Friedrich, "Structural
characterization of blotting membranes and the influence of membrane
parameters for electroblotting and subsequent amino acid sequence
analysis of proteins", Electrophoresis 14: 831-838, 1993. A useful
reference if you plan to do protein sequencing of samples transferred
samples.
5. LeGendre, Nancy "Immobilon-P Transfer Membrane: Applications and
Utility in Protein Biochemical Analysis", BioTechniques suppl to vol 9:
788-805 (1990). This references deals specifically with transfer
conditions using Immobilon-P type membranes.
6. Tovey, E.R. and B.A. Baldo. 1987. Comparison of semidry and
conventional tank buffer electrotransfer of proteins from
polyacrylamide gels to nitrocellulose membranes. Electrophoresis 8:
384-387. This paper discusses quantitative yields of proteins of
different molecular weights using different transfer conditions.
7. Dunbar, B.S., Ed. 1994. Protein Blotting: A Practical Approach. IRL
Press at Oxford University Press, Oxford, England. A great guide to
blotting techniques, including visualization, immunological techniques,
and sequence analysis.
Semidry Blotting -
General References