Thermo Scientific OWL HEP-1, Руководство по эксплуатации и техническому обслуживанию

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8. Alternatively, you can place the filter paper in a box with buffer, toss in
the gel, and drain the box until the gel falls to the pad. Add a few mL
of buffer to the filter pads already in the bottom chamber. Then pick
up the paper and gel and layer them on the filter stack.
9. Add a few mL of buffer to the gel, and gently layer the membrane, as
you did the gel.
10. Repeat with three more pieces of filter paper.
11. Holding the stack, drain off all excess buffer from the plate. Wipe away
any droplets around the edge of the stack.
Semidry Electroblotter 2-5 Thermo Scientific
Section 2
Setting Up
Gel Sandwich
Assembly (continued)
Anode
Membrane
Gel
Cathode
Transfer
Filter
Paper
Filter
Paper
Figure 2-1. Assembly Order