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Low-volume measurements with SkanIt Software

Creating a new measurement session

Thermo Fisher Scientific

Thermo Scientific μDrop and μDrop Duo Plates User Manual

7

Figure 6.

Selecting the plate template

All existing sample types are available for the layout (see

) and calculations.

Figure 7.

Example layout of μDrop Plate containing only blanks

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Содержание mDrop

Страница 1: ...Cat No N12691 Rev 4 0 2020 Thermo Scientific μDrop and μDrop Duo Plates User Manual ...

Страница 2: ...ty of their respective holders Disclaimer Thermo Fisher Scientific reserves the right to change its products and services at any time to incorporate technological developments This manual is subject to change without prior notice as part of continuous product development Although this manual has been prepared with every precaution to ensure accuracy Thermo Fisher Scientific assumes no liability fo...

Страница 3: ...GO Thermo Scientific Varioskan LUX Safety and Special Notices Make sure you follow the precautionary statements presented in this guide The safety and other special notices appear in boxes The following symbols and markings appear in this user manual WARNING Biohazard risk WARNING Risk of injury to the user or users CAUTION Risk of damage to the instrument other equipment or loss of performance or...

Страница 4: ...his Guide ii Thermo Scientific μDrop and μDrop Duo Plates User Manual Thermo Scientific Contacting Us For the latest information on products and services visit our website at www thermofisher com platereaders ...

Страница 5: ...ssions 6 Placing the samples 10 Cleaning 10 Cleaning verification 10 Chapter 3 Cuvette measurements with SkanIt Software 13 Placing the cuvette 13 Creating a new measurement session 14 User made sessions 14 Chapter 4 Setting up a measurement from the instrument 17 Chapter 5 Maintenance 19 Regular and preventive maintenance 19 μDrop and μDrop Duo Plates care 19 Cleaning the μDrop and μDrop Duo Plat...

Страница 6: ...Contents iv Thermo Scientific μDrop and μDrop Duo Plates User Manual Thermo Scientific ...

Страница 7: ...le volumes and one for cuvettes The low volume area of the μDrop and μDrop Duo Plates is compatible with an 8 channel pipette to enable easy pipetting of the samples to the μDrop and μDrop Duo Plates It can be used for endpoint and spectral measurements The cuvette slot is compatible with standard rectangular cuvettes with a stopper It can be used for endpoint spectral and kinetic measurements Int...

Страница 8: ...al pathlength for the sample is fixed to 0 5 mm The verified pathlength for each μDrop Plate is indicated on the quality control measurement report delivered with the μDrop Plate The μDrop Duo Plate has two low volume measurement areas on the left hand side see Figure 4 The verified pathlengths for each μDrop Duo Plate are indicated on the quality control measurement report delivered with the μDro...

Страница 9: ...rop and μDrop Duo Plates User Manual 3 Figure 2 An open μDrop Plate showing the top slide 1 bottom slide 2 sample positions A2 H3 2 x 8 matrix 3 low volume area 4 and cuvette slot 5 Figure 3 A closed μDrop Duo Plate showing the low volume measurement area 1 adjustment screw 2 and cuvette measurement point 3 ...

Страница 10: ...top slides 1 bottom slides 2 sample positions two 2 x 8 matrixes 3 low volume area 4 and cuvette slot 5 The measurement sessions for Multiskan SkyHigh Multiskan Sky Multiskan GO and Varioskan LUX can be created using Thermo Scientific SkanIt Software It is also possible to measure the μDrop and μDrop Duo Plates with Multiskan SkyHigh and Multiskan Sky internal software ready made protocols ...

Страница 11: ... sessions Create your own user defined sessions Ready made sessions The ready made sessions for commonly used assays can be downloaded from the SkanIt Cloud Library You can access the Cloud Library sessions if you have an Internet connection Figure 5 SkanIt Cloud library For more details about the SkanIt Cloud Library refer to the Thermo Scientific SkanIt Software for Microplate Readers Technical ...

Страница 12: ...of 0 05 cm 0 5 mm In the case of the μDrop Plate the pathlength provided in the certificate in cm has to be used in the Lambert Beer s equation to calculate the concentration of the target molecules In the case of the μDrop Duo Plate two pathlengths are provided one for each 2x8 matrix and these values need to be separately used in the calculations for the unknown samples located in each matrix In...

Страница 13: ... Thermo Fisher Scientific Thermo Scientific μDrop and μDrop Duo Plates User Manual 7 Figure 6 Selecting the plate template All existing sample types are available for the layout see Figure 7 and Figure 8 and calculations Figure 7 Example layout of μDrop Plate containing only blanks ...

Страница 14: ... types can be used with the μDrop and μDrop Duo Plates Note that long kinetic measurements or incubation are not recommended due to evaporation The μDrop and μDrop Duo Plates can be used in the whole wavelength range of the instrument in question Figure 9 Protocol example Note The μDrop and μDrop Duo Plates support only photometric measurements Note The μDrop and μDrop Duo Plates do not support di...

Страница 15: ...lue at 260 nm after blank subtraction A320 absorbance value at 320 nm after blank subtraction PATHLENGTH_CM pathlength value of the μDrop Plate in cm For the μDrop Duo Plate if different pathlengths are reported for each 2 x 8 matrix then two separate calculations of concentrations need to be carried out To accomplish that two different sample groups can be for instance created in SkanIt Software ...

Страница 16: ...on as possible after pipetting especially when using a single pipette With smaller sample volumes a larger evaporation effect can be observed due to changes in concentration 1 Open the lid covering the dotted sample area see Figure 2 on page 3 and Figure 4 on page 4 2 Pipette the samples into the small wells You can simultaneously measure a maximum of 16 or 32 samples when using the μDrop or μDrop...

Страница 17: ... of a well cleaned sample position should be below 0 050 Abs with wavelengths above 260 nm If the absorbance level is too high clean the μDrop or μDrop Duo Plate thoroughly For further instructions refer to Cleaning the μDrop and μDrop Duo Plates on page 19 A spectral measurement is also a suitable tool to verify for example traces of DNA in the sample positions ...

Страница 18: ...2 Low volume measurements with SkanIt Software Cleaning 12 Thermo Scientific μDrop and μDrop Duo Plates User Manual Thermo Fisher Scientific ...

Страница 19: ...m window 4 mm Figure 11 Examples of an acceptable 1 and unacceptable 2 stopper Placing the cuvette The cuvette is placed into the cuvette slot with the stopper pointing towards the adjustment screw and the reading window facing upwards see Figure 12 Note Use only cuvettes with stoppers for example Hellma cuvettes Article No 114B 10 40 or 115B 10 40 Note Use only quartz cuvettes black wall cuvettes...

Страница 20: ...ommended 1 Pipette the sample into the cuvette in a sufficient volume 2 Close the stopper and check that there are no visible air bubbles or superfluous particles present in the sample 3 Place the cuvette into the cuvette slot and tighten the adjustment screw see Figure 12 Ensure that there are no air bubbles in the measurement location white spot see Figure 1 on page 2 Creating a new measurement ...

Страница 21: ...The blank is added to the G11 well of the first plate in the layout see Figure 13 and Figure 15 The unknown samples are added to the following plates also to position G11 see Figure 14 and Figure 16 Add plates by clicking Add new in the Plate Layout ribbon All existing sample types are available for the layout and calculations Figure 13 Cuvette layout of μDrop Plate with a blank sample cuvette Fig...

Страница 22: ...tometric measurement types can be used with the cuvette on the μDrop and μDrop Duo Plates Settle delay Varioskan LUX only Use the settle delay with the μDrop and μDrop Duo Plates to let the liquid stabilize in the cuvette before measurement 3 Results All available calculation methods may be used as with normal microplates For more details about SkanIt Software refer to the Thermo Scientific SkanIt...

Страница 23: ...n SkyHigh Technical Manual Cat No N21872 To measure the μDrop or μDrop Duo Plate 1 Load the μDrop or μDrop Duo Plate to the instrument If the plate carrier is in push the power button to drive it out When measuring a cuvette place the cuvette into the cuvette slot For instructions see Placing the cuvette on page 13 2 Access the measurement from the Home screen of the instrument Note Before startin...

Страница 24: ...tific μDrop and μDrop Duo Plates User Manual Thermo Scientific 3 Select the μDrop Plate or μDrop Duo Plate plate template 4 Define the measurement parameters 5 Tap Start to start the measurement 6 View the results of the measurement 7 Export the results if desired ...

Страница 25: ...se disposable gloves 2 Wipe the samples with a dry lens tissue or soft cloth The lens tissue must not contain silicon 3 Clean both surfaces in contact with the sample of the low volume area with a lens tissue dampened with deionized distilled laboratory water 4 If necessary clean also both outside surfaces of the sample area with a dry lens tissue or a soft cloth dampened with deionized distilled ...

Страница 26: ...op Duo Plate decontaminate the μDrop or μDrop Duo Plate Refer to Decontamination procedure on page 20 CAUTION Do not use any solutions containing high concentrations of oxidative chemicals such as hypochlorites and peroxides or strong bases on any of the anodized aluminum surfaces as this may cause permanent damage to the finish WARNING Ensure that the bottom of the μDrop or μDrop Duo Plate is dry...

Страница 27: ...ide SQTM 1 64 Decon 90 min 4 To decontaminate the μDrop or μDrop Duo Plate 1 Prepare the decontaminant 2 Clean the low volume area Ensure that you are wearing disposable gloves Refer to Cleaning the μDrop and μDrop Duo Plates on page 19 3 Disinfect the outside of the μDrop or μDrop Duo Plate or remove stains using a cloth dampened with the decontaminant solution 4 Place the μDrop or μDrop Duo Plat...

Страница 28: ...e chemicals follow the waste management procedures on the μDrop or μDrop Duo Plate to ensure that there is no risk of contamination Follow laboratory and country specific procedures for biohazardous or radioactive waste disposal Use the recycling operators familiar to you for the original packaging and packing materials For more information contact your local Thermo Fisher Scientific representativ...

Страница 29: ...ecifications Table 1 General specifications Overall dimensions 127 8 mm W x 85 5 mm D x 14 5 mm H 5 0 W x 3 4 D x 0 57 H Weight 0 161 kg 0 4 lbs Low volume function μDrop Plate 16 measurement positions in a 2 x 8 matrix μDrop Duo Plate 2 x 16 measurement positions in two 2 x 8 matrixes Sample volume 2 μl to 10 μl Cuvette function Cuvette with stopper Dimensions 12 5 W x 12 5 D x 47 54 H mm Beam ce...

Страница 30: ...6 Technical specifications General specifications 24 Thermo Scientific μDrop and μDrop Duo Plates User Manual Thermo Scientific ...

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