Chapter 6
HAZARDS, PRECAUTIONS AND LIMITATIONS OF USE
6-4
MR23i
Thermo Fisher Scientific
Once the rotor is clean, rinse it in running water, preferably distilled.
Dry the rotor with a soft absorbant non-woven cloth or tissue.
Drying may be finished off with warm air jet (e.g. a hair dryer).
MAKE CERTAIN THAT THE POCKETS ARE WELL DRIED.
For swing-out rotors, be sure to clean the grease from the trunni-
ons and replace it with a small amount of fresh grease. This will
ensure that the buckets swing freely.
The vast majority of apparent imbalance problems arise from the
failure of the user to clean and grease the trunnions and not from
instrument error.
Pay particular attention that no deposit remains in the bottom of the
pocket because the pressure of a flask or tube from above during
centrifugation will significantly increase the chances of corrosion.
NEVER LEAVE A DAMP ROTOR ON A METAL SURFACE, PAR-
TICULARLY STAINLESS STEEL because an electrochemical
reaction could take place with the aluminium or magnesium in the
rotor.
CONTAMINATION HAZARDS
Our centrifuges are likely to be used in medical research where
hazardous substances, including radioactive chemicals, are fre-
quently found (see also
Chapter 6: “HAZARDS, PRECAUTIONS
AND LIMITATIONS OF USE” Section “BIOLOGICAL RISKS”
ALWAYS USE THE APPROPRIATE DECONTAMINATION PRO-
CEDURES WHERE THE ROTOR IS EXPOSED TO THESE CHE-
MICALS.
Examples of commonly used techniques are outlined below. The
information is given as a guide only. It is the responsibility of the
owner to use the most suitable procedure.
The rotor should always be completely disassembled before being
subjected to heat and after external chemical cleaning.
Seals, tubes and plastic components should be decontaminated
with the method most suitable for them which might not be the
same as for the rotor.
Disinfection
Alcohol (70% ethanol or isopropanol) applied for 10 minutes is
ideal for bacteria and viruses.
Autoclave rotors for 20 minutes at 120°C to destroy micro-orga-
nisms.
Rotor lids must be disassembled from rotor bodies. ‘O’ rings,
autoclaved separately, should be replaced when deformed.
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