Thermo Scientific 11174729, Инструкция по эксплуатации

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Chapter 6
HAZARDS, PRECAUTIONS AND LIMITATIONS OF USE
Thermo Fisher Scientific MR23i 6-17
• A ventilated centrifuge (without built-in refrigeration) perma-
nently exhausts into the room air which has passed through its
bowl while a refrigerated (or thermostated) centrifuge contains
a relatively sealed bowl. Thus the risks from the spread of aero-
sols are minimised in a refrigerated centrifuge and significant in
a ventilated centrifuge.
• Aerosols are produced naturally when using uncapped tubes or
bottles. They are also produced when a container deforms or
breaks provoking a smaller or greater sample leak. We draw
your attention to the use of tubes used in angle rotors. Even if
the liquid level does not touch the cap when in a static state, it
could do so during centrifugation and leak. You should there-
fore follow the instructions of the tube supplier concerning the
fill level and sealing of the tube.
Once a biological risk is known or suspected, samples should be pla-
ced in a sealed container. Should such containers not be available,
sealed accessories should be used such as buckets or angle rotors
with sealed lids.
• Duration of aerosol presence: when aerosols are created in a
centrifuge bowl it persists in the air for a period of 3-5 minutes
after the rotor stops moving. Indeed, the action of opening the
lid itself provokes the dispersion of aerosols in the environment.
• When an elevated risk of aerosols or of breakage is perceived
the accessories should be handled using gloves and opened in
an environment ensuring the protection of the operator (safety
cabinet, glove chamber, wearing a mask ...), even if they are
apparently sealed.