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PI-MTE System Manual
Issue 6
Figure 5-1: Block Diagram of Signal Path in System
Whether or not the data is displayed and/or stored depends on the data collection
operation (Focus or Acquire) that has been selected in the application software. In
WinView, these operations use the Experiment Setup parameters to establish the
exposure time (the period when signal of interest is allowed to accumulate on the
CCD).
•
In Focus mode, the number of frames and accumulations settings are ignored. A
single frame is acquired and displayed, another frame is acquired and
overwrites the currently displayed data, and so on until Stop is selected. No
frames of data are stored. However, when Stop is selected, the File Save
function can be used to save the currently displayed data. This mode is
particularly convenient for familiarization and setting up. For ease in focusing,
the screen refresh rate should be as rapid as possible, achieved by operating
with axes and cross-sections off, and with Zoom 1:1 selected.
•
In Acquire mode, every frame of data collected can be automatically stored (the
completed dataset may include multiple frames with one or more
accumulations). This mode would ordinarily be selected during actual data
collection. One limitation of Acquire mode operation is that if data acquisition
continues at too fast a rate for it to be stored, data overflow will eventually
occur. This could only happen in Fast Mode operation.
The remainder of this chapter begins by discussing the system on/off sequences. Then
the First Light procedure for imaging applications is presented. This procedure provides
step-by-step instructions about how to initially verify system operation.
Finally, the last three sections discuss factors that affect exposure, readout, and
digitization of the incoming signal. By understanding these factors and making
adjustments to software settings, signal quality can be maximized.
For information about synchronizing data acquisition with external devices, refer to
, on page 62, and
External Sync with Continuous Cleans
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