Revised 03/25/2020
17
2.)
If you are the last user of the day
:
a.)
Remove the LN2 dewar
, return the LN2 to the large dewar, and place the
microscope dewar
upside-down on its stand
. Place a towel below the cold
finger on the microscope to collect condensing moisture.
b.)
Run the cryo cycle
, located in the flap-
out panel from the “Vacuum” control
panel. Standard settings are “Duration” = 300 min, “Start after” = 10 min.
Log off your user account and log in as the vacuum monitor:
username = “
vacuumuser
”, password = “
vacuum
”
c.) The Vacuum Logger software should start automatically.
Click “
Log
” →
“
Start
” to begin logging the vacuum levels. C
reate a new file using today’s
date
(mm-dd-yyyy) as the file name (include zeros in the day/month).
VIII. Troubleshooting
1.)
No beam is visible
There are several possible causes for no beam being visible. Try the following
steps until a beam can be found:
a.) Make sure that no objective or SA aperture is inserted to block the beam.
b.)
Decrease the magnification (RC “Magnification”).
c.) Move the specimen stage (RC trackball) in case a grid bar or other non-
electron-transparent region is blocking the beam path.
