Revised 03/25/2020
16
c.) If artifacts persist, a
new gain reference
must be collected.
To prepare a gain reference:
i.) Move to an
empty location
, such as a
large hole in the support film. This
empty region
must cover the entire
CCD field of view
.
ii.)
Spread the beam
until it illuminates
at
least half of the viewing screen
.
iii.) Lift the
screen
and click “
Gain Acq.
”
in the “Bias/Gain” flap-out panel. (Or
select “Camera” → “Prepare Gain
Reference” in Digital Micrograph.)
VII. End of Session
1.) Leave the
microscope in the standard condition
for the next user:
a.)
Remove
any
objective
or
SA aperture
; leave
condenser
aperture
inserted
(in, out, out).
b.) Leave the
column valves closed
.
c.) Place the v
iewing screen down
; cover the window with the rubber mat.
d.) Switch the
filament off
(“Filament” button in the “Filament” panel).
e.) Leave the
magnification
in the
SA range
(preferably 2400×). This is
essential to maintain stable objective lens current and prevent thermal drift for
the next user.
f.)
Remove the sample holder
(be sure to
reset the stage
first), remove your
sample, and
return the holder to the microscope
. See section III for details.
g.)
Check the reservation system
to see if another user is scheduled after you.
h.) If you are
not the last user
of the day,
fill the LN2 dewar
.
i.)
Sign out
of the reservation system and leave any relevant comments. If
running the cryo cycle, please indicate this in the comments.
