Revised 03/25/2020
18
d.)
Check that the C2 lens (LC “Intensity”) is not severely under- or overfocused,
spreading the beam to a point that it is too dim to see. The optimal C2 setting
will vary with magnification but is typically 30
–
60%.
e.) If no beam is still visible, try loading a previously saved gun alignment file.
Alignments can be loaded through the “Alignments” control panel, typically
founding under the “Align” tab. Open the flap-out menu, go to the “File” tab,
and select a saved alignment file
. Select “Gun” from the “Available” list by
either double clicking or using the < button, then click apply. If desired,
alignments for other systems/modes of the microscope can also be loaded by
moving the appropriate labels to the “Selected” list and pressing apply.
2.)
One of the control pads (or z axis or tilt buttons) is unresponsive
Re-initialize the pad by unplugging the USB cable from the back of the PC
and re-inserting it. The right plug connects to the right panel, left to the left.
Always note that this occurred in the instrument log
. Reinitializing the
panels may randomly activate functions on that panel, so check for changes
in spot size, diffraction mode, etc. after plugging it back in.
If no direct
List of saved
alignment files
Microscope
systems/modes
with available
saved settings.
Selected settings
(will be loaded
when “Apply” is
clicked.)
