CSB CryoEM Facility TF20 Operating Instructions SEC
05/02/16
Procedure
:
During specimen insertion and retraction you should
not
need to use considerable force to load
specimens.
Do not force or jam anything
.
It could damage the compustage and/or holder. Contact
the EM manager if you notice anything unusual (noises, smells, sounds, anything out of the ordinary
could signal trouble. When in doubt, please ask).
1.
Make sure that the Column Valves are closed (Setup Tab, Column Valves closed is yellow).
2.
Verify that the red light on the compustage is off.
3.
Make sure the stage is at 0,0,0 in X, Y and Z by going to the Search Tab, Stage 2 menu, flaput
window, and click on Holder. This will 'Home' the stage.
4.
Turn on the Turbo pump on the Setup Tab (click on Turbo On, the button will go from grey to
orange and when the turbo is ready, it will be yellow).
5.
Get the RT holder, remove the plastic covering at the end.
6.
With the tool that's in the back part of the mounting station, place it in the small orifice in the tip
area (see picture) and carefully lift the clamp (from 3 o'clock to 12 o'clock).
