SMARTer™ ICELL8® cx Single-Cell System User Manual
(061918)
takarabio.com
Takara Bio USA, Inc.
Page 14 of 35
B.
Setup and Installation
Your TBUSA Service Engineer will unpack and install your ICELL8 cx and explain the basic operation
of the system. They will use material from the ICELL8 cx Installation Kit to qualify the instrument after
installation and will leave reusable and/or remaining materials at your site. Table III below lists the
SMARTer ICELL8 cx Installation Kit components.
Table III. ICELL8 cx Installation Kit components.
Component
ICELL8 Alignment Chip
ICELL8 Chip Alignment Film
Imitation Master Mix with UV Dye & Rox (45 ml)
384-Well Source Plate
Blotting Paper (Pack 10)
384-Well Source Plate Seal (2)
The computer that runs the ICELL8 cx is equipped for Wi-Fi access, but it is disabled. If you choose to
activate Wi-Fi, we recommend that you seek support from your institution’s IT personnel to avoid
interfering with instrument operation.
NOTE:
To avoid contaminating your PCR, do not install the ICELL8 cx in an area that could contain
high-copy DNA or amplicons from previous PCR experiments.
C.
Required Equipment and Supplies from Your Lab or Other Suppliers
Wash Solution
•
500 ml of 0.2% sodium hypochlorite solution, made from reagent-grade sodium hypochlorite in
deionized, filtered water (Milli-Q or Elga system or equivalent; 0.2-µm filtration)
Reagents for ICELL8 cx Containers
•
Water Container:
fill with deionized filtered water (Milli-Q or Elga system or equivalent;
0.2-µm filtration)
•
Wash Container:
fill with Wash Solution
Other Reagents and Materials
•
Prepared samples
•
Instructions for preparing samples and reagents for dispensing with the ICELL8 cx (provided
with application-specific user manuals)
•
70% isopropanol
Equipment
•
Ice bucket and/or cold rack
•
Calibrated pipette and nuclease-free, aerosol-resistant tips (8-channel and repeating pipettes are
very useful in this procedure)
•
Vortex
•
Centrifuge with rotor capable of spinning microwell plates at 3,220
g
