Sutter Instrument MP-285, Основные операции

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MP-285 SYSTEM ROE BASIC OPERATIONS – REV. 4.04 (20210122)
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3. MICROSCOPIC MANIPULATION
3.1 Some Basic Information
Assuming you have already tried gross movement of the MP-285/M as outlined above (Initial
Setup and Gross Movement) then you understand most of the features necessary for
microscopic manipulation using your MP-285/M. The controller makes two changes to
implement fine movement:

The number of microsteps commanded per revolution of the ROE is reduced.

The size of the microsteps produced by the stepping motor (minimal resolution) is
reduced from 0.2 to 0.04 µm.
These two changes give you a wide range of control over the amount of movement
commanded by a single turn of an ROE knob. In the gross movement mode, you are
commanding slightly over 700µm/turn. In the precise movement mode, you can command as
few as 20 µm/turn.
As with the other portions of this User’s Manual, it is hoped that enough information has
been provided to get the average user “micromanipulating” with a minimum of time spent
covering unnecessary detail. If you find yourself needing additional detail on any of the items
covered, please consult the appropriate topic in the Reference Manual.
3.2 SETUP Selection
In order for the MP-285 to make precise movements and have a greater amount of control
over the movement, you instruct the controller to produce fewer microsteps (the minimal
increment of manipulator movement) per rotation of the ROE knobs. Using simple
commands, you choose one of four different “Setups” that vary the number of microsteps per
turn of the ROE knob over a range of 512 to 5120.
The following steps outline how to use your MP-285 to manipulate a pipette into position in
the field of view of your microscope. In doing so, you will learn how to switch from one setup
to another. The same procedure can also be used as the final steps of manipulator setup and
installation assuming the MP-285 is mounted on or near your microscope.
1.
Turn on the manipulator power; while still in the main menu press <1>. This selects
Setup 1 and allows for rapid positioning of the pipette in your microscopic field of view.
The upper left-hand corner of the display should now read “S
⇒ 1”.
2. Press <MOVE> to allow movement; remember that you cannot make movements unless
you see the movement screen displayed
3. Focus the microscope to a height slightly above your preparation.
4. Using the X and Y-axes, move the pipette tip to a position above the middle of your field
of view. In most cases, the first indication that the pipette is in the correct approximate
location is an out-of-focus shadow. You may need to work at a lower microscopic power to
visualize the tip.
5. Once you can see the shadow of the tip above your preparation, use the Z-axis control (top
ROE knob) to move the pipette down into focus. If you focused above your preparation,
you should not crash the pipette into your dish and/or preparation.