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MP-285 SYSTEM ROE BASIC OPERATIONS – REV. 4.04 (20210122) 

This four-hole pattern is also compatible with the MP-285 rotary base, part #285RBI and the 
mounting adapter plate, # 285210 (see the Reference Manual for further instructions). 
While it is possible to insert and tighten all four mounting screws with the MP-285 in one 

position, you may find it convenient to connect and power up the manipulator and position 
the X-axis for the best access to the screw holes. 

2.4

 

Mounting Headstages 

The most stable way to mount a headstage is to fasten it directly to the Z-axis slide. The 
mounting hardware included with the MP-285 is designed to eliminate the need for custom 

machining of headstage and pipette holders. For example, Axon Instruments’ new 203B 
headstage mounts directly to the rotating dovetail on the MP-285 Z-axis. Other headstages 
require use of the dovetail mounting plate:  

 

dovetail mounting plate:

 

         2 middle (D) holes

 

EPC:  1-1/16" x 2-7/32"

 

         (2 holes, A & B)

 

CV-4: 1-1/8" x 1-15/16"

 

          (4 holes, B & C)

 

A

 

B

 

C

 

C

 

C

 

D

 

D

 

 

Figure 2-6. Headstage mounting plate 

The plastic portion of the plate provides electrical isolation while allowing for a direct mount 
of Axon Instruments’ CV-4 headstage or Heka Electronics’ EPC-7, 8, or 9 headstages. The 

user can drill additional holes to allow the plate-mount installation of other headstages. 
Headstage mounting instructions can be found in the Reference Manual. 
Whether using the Axon 203B Headstage or another headstage mounted on the dovetail 
mounting plate, the respective male dovetail is inserted into the female dovetail on the MP-

285 Z-axis. Slide the dovetail mounting plate/headstage assembly into the rotating-dovetail 
base and secure it by tightening the dovetail clamp set screw with a Phillips-head 
screwdriver. 

Содержание MP-285

Страница 1: ...tal Drive Novato CA 94949 Voice 415 883 0128 Web www sutter com Fax 415 883 0572 Email info sutter com MP 285 Micromanipulator System Including Model MP 285A Controller ROE Basic Operations Rev 4 04 2...

Страница 2: ...MP 285 SYSTEM ROE BASIC OPERATIONS REV 4 04 20210122 ii Copyright 2021 Sutter Instrument Company All Rights Reserved...

Страница 3: ......

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Страница 5: ...esigned SAFETY WARNINGS AND PRECAUTIONS Electrical Operate the MP 285 using 110 240 V AC 50 60 Hz line voltage This instrument is designed for use in a laboratory environment that has low electrical n...

Страница 6: ...This unit is not designed for operation at altitudes above 2000 meters nor was it tested for safety above 2000 meters DO NOT CONNECT OR DISCONNECT THE CABLES BETWEEN THE CONTROLLER AND THE MECHANICAL...

Страница 7: ...zo impact microinjection call for the use of mercury in the micropipette tip The use of any hazardous materials with any Sutter Instrument s instrument is not recommended and if undertaken is done so...

Страница 8: ...MP 285 SYSTEM ROE BASIC OPERATIONS REV 4 04 20210122 vi This page intentionally left blank...

Страница 9: ...otating Dovetail Base 8 2 6 Minimizing Electrical Noise 9 3 MICROSCOPIC MANIPULATION 11 3 1 Some Basic Information 11 3 2 SETUP Selection 11 3 3 Changing Minimal Resolution 12 4 PULSE MOVEMENT 15 5 HO...

Страница 10: ...r connections 4 Figure 2 5 Manipulator base dimensions 6 Figure 2 6 Headstage mounting plate 7 Figure 2 7 Mounting the headstage on the manipulator s Z axis 8 Figure 2 8 Adjusting the headstage angle...

Страница 11: ...h emphasis on using the ROE Rotary Optical Encoder for user control These steps are in many ways identical to the steps performed any time one sets up an MP 285 for use at a microscope Many users will...

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Страница 13: ...dovetail headstage mounting plate with plastic isolation plate Metal dovetail rod clamp 2 5mm hex wrench for removing the shipping screws 7 64 hex wrench for adjusting the rotating dovetail base MP 28...

Страница 14: ...cessary strain 1 Connect the power cord to the MP 285 controller Make certain that the voltage selector next to the power cord socket on the back of the MP 285 controller has been set to the appropria...

Страница 15: ...three axes Before the unit was shipped from Sutter its absolute origin location 0 0 0 was set at the center of travel of each axis This same location was also defined as Home This means that you can...

Страница 16: ...the bearing ways A supply of four precision thickness washers is included with your MP 285 manipulator To limit misalignment you should use one on each of the four mounting screws between the MP 285 m...

Страница 17: ...y to the rotating dovetail on the MP 285 Z axis Other headstages require use of the dovetail mounting plate dovetail mounting plate 2 middle D holes EPC 1 1 16 x 2 7 32 2 holes A B CV 4 1 1 8 x 1 15 1...

Страница 18: ...igure 2 8 Adjusting the headstage angle To adjust the rotary dovetail to your desired angle 1 Loosen the brake set screw to rotate the headstage or pipette holder Failure to loosen the screw may lead...

Страница 19: ...ulator will largely eliminate this noise source Ground tabs are located on each motor housing for this purpose It should be noted that the manipulator produces negligible electrical noise when it is n...

Страница 20: ...MP 285 SYSTEM ROE BASIC OPERATIONS REV 4 04 20210122 10 This page intentionally left blank...

Страница 21: ...microsteps the minimal increment of manipulator movement per rotation of the ROE knobs Using simple commands you choose one of four different Setups that vary the number of microsteps per turn of the...

Страница 22: ...the pipette If you wish to have even better control press the COARSE FINE button on the ROE see below Four factory installed Setups are selected from the main menu by pressing the corresponding numbe...

Страница 23: ...3 2 Microns per ROE turn settings Setup Number Microns per ROE turn at 0 2 m 0 04 m 1 716 8 143 36 2 1024 204 8 3 512 102 4 4 102 4 20 48 Thus you have a relatively wide range of precision of movemen...

Страница 24: ...MP 285 SYSTEM ROE BASIC OPERATIONS REV 4 04 20210122 14 This page intentionally left blank...

Страница 25: ...e unit is in continuous mode Pulse movement can be most easily observed under a microscope If you have already configured your microscope for micromanipulation as detailed in section Microscopic Manip...

Страница 26: ...1 The speed of pulse movement is 5000 microsteps second for all Setups As there are two different resolutions this translates to the same two different speeds for each setup 1 m sec at coarse resoluti...

Страница 27: ...the cursor indicated by the arrow to New Home Press ENTR the New Home menu will appear 5 Press ENTR to accept the default speed and resolution You will be back in the movement screen 5 2 Moving to the...

Страница 28: ...xtend the same amount If you use a rotating device to position the manipulator try to install it such that rotation is up against a hard stop when the manipulator is in the operating location Note tha...

Страница 29: ...advantageous to define this location as the absolute zero 0 0 0 for the MP 285 controller Once this is done it is straightforward to know where you are when you are looking at the coordinate display...

Страница 30: ...ent press MOVE to return to the movement screen 2 Press to enter the Robotics menu TAB 3X to Learn and press ENTR to gain access to Learn functions You will be issued the following warning 3 If you ha...

Страница 31: ...ogram To store your newly entered routine in a location other than program location 1 follow the steps detailed in the section Clear Program Location 1 for recording a new Robotic Series 6 5 Executing...

Страница 32: ...ogrammed a multiple step withdrawal pattern to move your pipette out through a circuitous route you may want to use a similar pattern to move your pipette into the working area This can be accomplishe...

Страница 33: ...gonal movements while in the movement screen 1 Press the remote NORM DIAG button on the encoder down and lit diagonal mode 2 The movement screen should now indicate that the controller is in diagonal...

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Страница 35: ...ipulator Furthermore one can control the direction of movement commanded by a given input Axes assignment is covered in the manual under Controller Configuration and is adjusted using the menu command...

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Страница 37: ...l void this warranty Warranty work will be performed only at the factory The cost of shipment both ways is paid for by Sutter Instrument during the first three months this warranty is in effect after...

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Страница 39: ...precautions v H home function 17 M mains fuses iii microscopic manipulation 11 movement amount per ROE turn 25 control of 25 N notes user 29 30 P precautions iii electrical iii pulse movement 15 R RO...

Страница 40: ...MP 285 SYSTEM ROE BASIC OPERATIONS REV 4 04 20210122 30 NOTES...

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