16
STEINDORFF
®
4.2.2.
The centering ring (Figure 28 and Figure 29)
Usually you needn’t the operation of centering. If necessary, please accord to the following steps:
1.
Place the specimen on the stage and focus it.
2.
Take out the eyepiece, replace it with the CT (the centering telescope), and inserted it into the viewing tube
without diopter adjustment.
3.
Make sure the matched phase contrast objective and light ring (in the phase contrast slider) have been in the
center of the light path.
4.
Using the CT to look the light ring’s image
①
and the phase contrast ring’s image
②
, if the light ring’s
image is not sharp, please shifting the CT’s ocular until you can see a clear image of the light ring
②
.
5.
Adjusting the bolts of the two centering holes
③
in the phase contrast slider by the screwdriver
③
until the
light ring center and the phase contrast center are coincided.
6.
The 10X and the 20X phase contrast objective use the same light ring on the phase contrast slider. So you
need to check the coincidence of the light ring center and the phase contrast center when changing the
objective. If having departure, you ought to center again.
If the light ring is centering incorrectly, you will fail to obtain the best viewing effect of the microscopy.
After removing or replacing a thick specimen, the light ring and the phase contrast ring are likely to deviate
each other, which will result in a decline of the image contrast. So if happened, please repeat the steps as
above.
If the container or the cover flip which used to place the specimen is not flat, it maybe need to repeat the
centering steps for obtaining a more contrast effect. Please center the light ring by the phase contrast
objective, according to the sequence of low to high magnification.
Figure 28
Figure 29
