8
Note:
1.) Blue light bands may only be seen in a darkened room or by shielding the unit from light by a dark
cloth or paper.
2.) In order to conserve the blue light lamp, the light will be there for 10 seconds only, and then switch
off again by its own. Press the button for 3 seconds to switch constant light on.
3.) By its very nature during electrophoresis the application of current through a gel leads to a build up
of heat resulting in the accumulation of condensation within the runVIEW lid viewing pane. Excessive
levels of condensation impair visualisation of the nucleic acid bands within the gel. Condensation may
be cleared by using the fan extractor in runVIEW lid.
Using runVIEW as a blue light illuminator
We recommend use with the DNA loading and staining reagents ROTI
®
Load DNAstain1 or 2 for blue
light and UV-light excitation.
1.
With runVIEW placed on an even bench surface, switch it on using the ON/OFF button located at
the rear of the base unit.
2.
Using gloved hands, place the gel tray containing the gel onto the illumination platform within the
base unit and place the orange lid on top.
3.
Switch on the blue light by pressing the
button located on the front panel. Any present DNA
stained with ethidium bromide or ROTI
®
Load DNAstain should be immediately visible beneath the
runVIEW lid.
4.
Protective glasses are not necessary when viewing the blue light illuminator.
Note:
1.) Blue light bands may only be seen in a darkened room or by shielding the unit from light by a dark
cloth or paper.
2.) In order to conserve the blue light lamp, the light will be there for 10 seconds only, and then switch
off again by its own. Press the button for 3 seconds to switch constant light on.
Using runVIEW for DNA recovery
1.
Cast a gel featuring two rows of wells with one matching pair of the preparatory combs supplied,
before transferring the blue-light transparent gel tray to the PROfessional III tank located on the
base unit.
2.
Add sufficient buffer just to cover the gel, and remove the combs to load the DNA samples into the
upper row of wells ('Loading' tier).
3.
Replace the lid to connect the PROfessional III tank to the integrated power supply before applying
the voltage as described in SETUP MODE. Press the
button to start the run.
4.
Press the
to switch on the blue LED illuminator.
5.
Watch through the run
VIEW lid’s viewing pane as the samples migrate in real-time to the second
row of wells ('extraction' tier).
6.
Once the DNA bands of interest enter the 'extraction' tier, simply stop the power supply, remove the
lid and harvest the DNA by pipette.
7.
Upon harvesting, measure the volume obtained from the extraction well by pipette before
performing ethanol precipitation using 1/10
th
volume of 3 M Sodium Acetate and 2x volumes of ice-
cold 100 % ethanol. Spin using a micro centrifuge
for 10’ at maximum rpm.
8.
Decant supernatant and perform a second centrifugation for 10 min. with ice-cold 70 % ethanol.
9.
Decant supernatant and dry the DNA pellet at room temperature or using a Speedy vac
10.
Once dry, resuspend the pellet in a small volume of distilled water or TE buffer, and store or use
accordingly.
