background image

 

10 

 
Combs 

Sample volume for a 5 mm thick gel 

 

4 + 2 

10 

12 

16 

20 

28 

35 

0.75 mm 

91 µl 

34 µl 

30 µl 

20 µl 

16 µl 

8 µl 

7 µl 

1.0 mm 

122 µl 

45 µl 

41 µl 

27 µl 

21 µl 

11 µl 

10 µl 

1.5 mm 

182 µl 

68 µl 

61 µl 

41 µl 

32 µl 

17 µl 

15 µl 

2.0 mm 

243 µl 

90 µl 

81 µl 

54 µl 

43 µl 

23 µl 

20 µl 

 
Wells 

4 + 2 

10 

12 

16 

20 

28 

35 

Thickness 

Art. No. 

Art. No. 

Art. No. 

Art. No. 

Art. No. 

Art. No. 

Art. No. 

0.75 mm 

3263.1** 

3266.1 

3269.1 

3270.1* 

3276.1 

3281.1* 

3283.1 

1.0 mm 

3302.1** 

3308.1 

3309.1 

3312.1* 

3317.1 

3332.1* 

3334.1 

1.5 mm 

3339.1** 

3347.1 

3350.1 

3365.1* 

3368.1 

3370.1* 

3372.1 

2.0 mm 

3373.1** 

3374.1 

3378.1 

3379.1* 

3381.1 

3382.1* 

3384.1 

*compatible with multi-channel pipettors  
**comb with especially broad wells + narrow marker well (for preparative gels) 
 

 
I. Accessories for ROTIPHORESE

®

 PROfessional I electrophoresis unit 

Special lid for runVIEW system, green and red fluorescence  

 

    

3663.1 

Power Supply Roth-MINI 

 

 

 

 

 

    

2907.1 

Power Supply EV1450   

 

 

 

 

 

    

CP56.1 

Further accessories see Professional I unit, Art. No. 2788.1. 
 
 

J. Accessories for ROTIPHORESE

®

 PROfessional II electrophoresis unit 

Special lid for runVIEW system, green and red fluorescence 

 

 

3680.1 

Power Supply Roth-MINI 

 

 

 

 

 

     

2907.1 

Power Supply EV1450   

 

 

 

 

 

     

CP56.1 

Further accessories see Professional II unit, Art. No. 2799.1. 
 
 

K. Additional Items and Reagents 

Levelling table 

 

N854 

Agaroses: 

Standard 

 

3810 

NEEO ultra-quality 

 

2267 

Agarose-Tablets 

 

HP67 

Broad Range (for all fragment lengths) 

 

T846 

GTQ (gene technique quality - for DNA elution) 

 

6352 

HR-PLUS (for fragments 100-3000 bp)  

 

HP30 

High Resolution (for small fragments 50-1000 bp) 

 

K297 

Low Melt (for gel elution and in-gel-applications, for fragments 500-20000 bp)   6351 
LM/PCR (Gel elution of fragments < 1500 bp) 

 

HP31 

Super LM (particularly low melting temp., for fragments 

 1000 bp) 

 

HP54 

Synergel

TM

 (Agarose additive for even better band resolution)  

 

0184 

Gel Loading Buffers: 

ROTI

Load DNA 6x (with Glycerol / Ficoll)  

 

X904 / X905 

ROTI

Load DNA short run 6x (with Glycerol) 

 

0095 

ROTI

Load DNA 1x (with Glycerol) 

 

0100 

ROTI

Load DNA short run 1x (with Glycerol) 

 

0099 

ROTI

Load DNA small (with Glycerol) 

 

HP03 

ROTI

Load DNA orange 1 (with Glycerol) 

 

HP04 

ROTI

Load DNA orange 2 (with Glycerol) 

 

HP05 

ROTI

Load DNA tricolor (with Glycerol) 

 

HP06 

Содержание 4849.1

Страница 1: ...zontal electrophoresis units from ROTH are designed for long term laboratory use and to obtain reproducible results Please spend a few moments reading the instruction manual thoroughly These units comply with the statutory CE safety rules 73 23 EEC Low voltage directive IEC 1010 1 1990 plus amendment 1 1992 EN 61010 1 1993 BS EN 61010 1 1993 Please verify that you received the unit completely and ...

Страница 2: ...ruction manual The lid is suitable to electrophoresis unit ROTIPHORESE PROfessional III Art No 4850 1 9958 1 ROTIPHORESE PROfessional runVIEW Base MINI 1 o 1x blue light illuminator adjustable to 2 different sizes including power cord o 1x special lid with spectral emission filter and extractor fan within its viewing pane for dyes with green and red fluorescence o Instruction manual The lid is sui...

Страница 3: ...able for dyes with green fluorescence e g the non toxic dye SYBR Green and red fluorescence e g ethidium bromide or the non toxic dye ROTI Gelstain Red Art No 0984 The blue light illuminator provides instant time saving visualisation of DNA RNA band migration No UV safety equipment required for blue light illumination For the ROTIPHORESE PROfessional III unit gel trays of 15 x 7 15 x 10 and 15 x 1...

Страница 4: ...late with wet hands 7 Do not connect to ground any of the power outputs or the buffer within the electrophoresis tank the power outputs should be only connected to an insulated electrophoresis tank equipped with a safety cover 8 Do not connect any power supplies in series or in parallel 9 Never open the back plate nor remove the cover otherwise an electric shock may result Repairs should only be m...

Страница 5: ...are partially shrouded for protection However when cleaning the main tank do not use cleaning brushes in the electrode area Ensure that the connectors are clean and dry before usage or storage RNAse Decontamination Clean the units with a mild detergent as described above Wash with 3 hydrogen peroxide H2O2 for 10 minutes Rinse with 0 1 DEPC diethyl pyrocarbonate treated distilled water Caution DEPC...

Страница 6: ...e Use to alternatebetween Voltage Current and Time Use or to adjust the value to the desired setting b Operation Mode Press to start electrophoresis The LED light next to the Start Stop button will light up to indicate the unit is in operation Use to monitor the remaining time and changes in current and voltage c Blue light There are two modes of blue light illumination for visualisation of nuclei...

Страница 7: ...he DNA loading and staining reagents ROTI Load DNAstain1 2 or 3 containing the non toxic fluorescent dye EvaGreen see below 5 Select your settings accordingly In order to run the system at constant voltage switch the mode button to the Voltage setting and alter the value to the desired setting as described in Set Up Mode the Volt LED will be illuminated by this stage 6 Use the same principle to ru...

Страница 8: ... shielding the unit from light by a dark cloth or paper 2 In order to conserve the blue light lamp the light will be there for 10 seconds only and then switch off again by its own Press the button for 3 seconds to switch constant light on Using runVIEW for DNA recovery 1 Cast a gel featuring two rows of wells with one matching pair of the preparatory combs supplied before transferring the blue lig...

Страница 9: ...oresis the application of current through a gel leads to a build up of heat resulting in the accumulation of condensation within the runVIEW lid viewing pane Excessive levels of condensation impair visualisation of the nucleic acid bands within the gel Condensation may be cleared by using the fan extractor in runVIEW lid G At the End of the Run Turn the power supply settings to zero turn off mains...

Страница 10: ...or ROTIPHORESE PROfessional II electrophoresis unit Special lid for runVIEW system green and red fluorescence 3680 1 Power Supply Roth MINI 2907 1 Power Supply EV1450 CP56 1 Further accessories see Professional II unit Art No 2799 1 K Additional Items and Reagents Levelling table N854 Agaroses Standard 3810 NEEO ultra quality 2267 Agarose Tablets HP67 Broad Range for all fragment lengths T846 GTQ ...

Страница 11: ...suitable for all PROfessional gel trays up to a size of 26 x 32 cm W x L When using gel trays up to a size of 10 x 10 cm 2 gels can be poured simultaneously PROfessional I and II The level bubble allows an optimal levelling of the gels Additional sealing is not necessary L Troubleshooting and Maintenance Each runVIEW system uses all solid state components and should require no maintenance or recal...

Страница 12: ...ircuit is broken in the electrophoresis system Check the connections to the power supply and within electrophoresis system to make sure the connection is intact check the electrodes to make sure they are intact Close the circuit by reconnecting the cables Press START STOP to restart the run High resistance due to tape left on a pre cast gel incorrect buffer concentration or insufficient buffer vol...

Страница 13: ...2020 Complete electrophoresis system ROTIPHORESE PROfessional runVIEW 4849 1 Electrophoresis Station ROTIPHORESE PROfessional runVIEW Base Suitable for Unit PROfessional III 4850 1 Electrophoresis Station ROTIPHORESE PROfessional runVIEW Base MINI 1 Suitable for Unit PROfessional I 9958 1 Electrophoresis Station ROTIPHORESE PROfessional runVIEW Base MINI 2 Suitable for Unit PROfessional II 9961 1 ...

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