9
TIP: For extractions performed with
samples of low DNA amount
, a small piece of dialysis
membrane may be inserted into the extraction wells ahead of elution. The paper may be washed by
changing the salt concentration to release the DNA, whereas for the membrane, reversal of the power
output cables in the base unit (i.e. red cable to the black outlet and the black cable to the red outlet)
and application of the voltage for 15-60 seconds, should release the DNA from the dialysis membrane.
For both techniques, the solution should be then removed from the extraction tier and ethanol
precipitation performed as described steps 7-10.
Note:
1.) Blue light bands may only be seen in a darkened room or by shielding the unit from light by a dark
cloth or paper.
2.) In order to conserve the blue light lamp, the light will be there for 10 seconds only and switch off
again by its own. Press the button for 3 seconds to switch constant light on.
3.) By its very nature during electrophoresis the application of current through a gel leads to a build up
of heat resulting in the accumulation of condensation within the runVIEW lid viewing pane. Excessive
levels of condensation impair visualisation of the nucleic acid bands within the gel. Condensation may
be cleared by using the fan extractor in runVIEW lid.
G. At the End of the Run
Turn the power supply settings to zero, turn off mains supply and disconnect the power leads.
Remove safety lid at the end of the run and take gel tray out of the chamber for staining.
Rinse the apparatus with distilled water only after the run (see section: B General Care and
Maintenance). Ensure that the connectors are clean and dry before usage or storage.
NOTE: Make sure to take of current and disconnect the electrophoresis unit from Power Supply
before opening the unit!
H. Accessories for ROTIPHORESE
®
-PROfessional III electrophoresis unit
Special lid for runVIEW system, green and red fluorescence
3077.1
Gel tray (w/o casting dams) 15 x 7 cm
3250.1
Gel tray (w/o casting dams) 15 x 10 cm
3251.1
Gel tray (w/o casting dams) 15 x 15 cm
3252.1
Gel casting dams (1 pair)
3262.1
Positive electrode
3247.1
Negative electrode
3248.1
