Roth 4849.1 Скачать руководство пользователя страница 4

 

 

 

Real-time electrophoresis for ROTIPHORESE

®

 PROfessional I and II units 

 
9958.1

 

/ 9961.1 ROTIPHORESE

®

 PROfessional runVIEW Base MINI  

 

The runVIEW base MINI consists of a blue light illuminator and a special lid, containing a spectral 
emission filter and extractor fan within its viewing pane.  

The lid is suitable for dyes with green fluorescence, e.g. the non-toxic dye SYBR

®

 Green, and red 

fluorescence, e.g. ethidium bromide or the non-toxic dye ROTI

®

Gelstain Red (Art. No. 0984).  

The illuminator is adjustable to 2 different sizes. It is suitable to the units ROTIPHORESE

® 

PROfessional I (Art. No. 2788.1) and ROTIPHORESE

® 

PROfessional II (Art. No. 2799.1). No UV 

safety equipment required for blue light illumination. 

Convenient power supplies are EV1450 (Art. No. CP56.1) or Roth MINI (Art. No. 2907.1).  

 

Operational (runVIEW Base MINI) 

 
 
 
 

 
 

USING THE HORIZONTAL GEL ELECTROPHORESIS UNITS

 

A. Safety Precautions

 

1.

 

Read the instruction manual thoroughly before use. 

2.

 

Never touch the power outlets with any conductive object (e.g. naked metal wire) other than 
properly insulated power supply cables. 

3.

 

Do not spill liquid or insert metal objects inside the power supply. 

4.

 

Never block the ventilation holes or place the unit in any enclosure unless there is adequate 
ventilation; never expose the power supply to a direct heat source. 

5.

 

Never touch any part of the power supply assembly (i.e. power supply, cables or electrophoresis 
tank) before switching OFF the power supply. 

6.

 

Never manipulate with wet hands. 

7.

 

Do not connect to ground any of the power outputs or the buffer within the electrophoresis tank; the 
power outputs should be only connected to an insulated electrophoresis tank equipped with a 
safety cover. 

8.

 

Do not connect any power supplies in series or in parallel. 

9.

 

Never open the back plate nor remove the cover, otherwise an electric shock may result. Repairs 
should only be made by the manufacturer or a service technician authorised by the manufacturer. 

10.

 

Never use this power supply if the safety cover is not in position correctly. 

11.

 

Do not use the unit if there is any sign of damage to the external tank or cover. Contact our 
technical service (++49-721-5606-172) immediately to replace or repair any damaged parts. 

12.

 

Never use the power supply in the presence of flammable or combustible material as fire or 
explosion may result.  

13.

 

Ensure that the power supply is only connected to an earthed power line. Do not cut and splice the 
power line. When removing the power cord from the wall, unplug it by holding the plug attachment 
and not by pulling the cord. Do not hold the plug with wet hands or gloves. 

14.

 

Please wear always protective gloves while working. 

15.

 

Do not fill the unit with running buffer above the maximum fill lines. 

16.

 

Do not move the unit when it is running. 

 

CAUTION:

 During electrophoresis very low quantities of various gases are produced at the 

electrodes. The type of gas produced depends on the composition of the buffer employed. To disperse 
these gases, make sure that the apparatus is run in a well ventilated area. 

 

Wave length (excitation) 

470 nm (blue) 

Ambient temperature 

15-40 °C 

Current 

100 

– 240 V 

Содержание 4849.1

Страница 1: ...zontal electrophoresis units from ROTH are designed for long term laboratory use and to obtain reproducible results Please spend a few moments reading the instruction manual thoroughly These units comply with the statutory CE safety rules 73 23 EEC Low voltage directive IEC 1010 1 1990 plus amendment 1 1992 EN 61010 1 1993 BS EN 61010 1 1993 Please verify that you received the unit completely and ...

Страница 2: ...ruction manual The lid is suitable to electrophoresis unit ROTIPHORESE PROfessional III Art No 4850 1 9958 1 ROTIPHORESE PROfessional runVIEW Base MINI 1 o 1x blue light illuminator adjustable to 2 different sizes including power cord o 1x special lid with spectral emission filter and extractor fan within its viewing pane for dyes with green and red fluorescence o Instruction manual The lid is sui...

Страница 3: ...able for dyes with green fluorescence e g the non toxic dye SYBR Green and red fluorescence e g ethidium bromide or the non toxic dye ROTI Gelstain Red Art No 0984 The blue light illuminator provides instant time saving visualisation of DNA RNA band migration No UV safety equipment required for blue light illumination For the ROTIPHORESE PROfessional III unit gel trays of 15 x 7 15 x 10 and 15 x 1...

Страница 4: ...late with wet hands 7 Do not connect to ground any of the power outputs or the buffer within the electrophoresis tank the power outputs should be only connected to an insulated electrophoresis tank equipped with a safety cover 8 Do not connect any power supplies in series or in parallel 9 Never open the back plate nor remove the cover otherwise an electric shock may result Repairs should only be m...

Страница 5: ...are partially shrouded for protection However when cleaning the main tank do not use cleaning brushes in the electrode area Ensure that the connectors are clean and dry before usage or storage RNAse Decontamination Clean the units with a mild detergent as described above Wash with 3 hydrogen peroxide H2O2 for 10 minutes Rinse with 0 1 DEPC diethyl pyrocarbonate treated distilled water Caution DEPC...

Страница 6: ...e Use to alternatebetween Voltage Current and Time Use or to adjust the value to the desired setting b Operation Mode Press to start electrophoresis The LED light next to the Start Stop button will light up to indicate the unit is in operation Use to monitor the remaining time and changes in current and voltage c Blue light There are two modes of blue light illumination for visualisation of nuclei...

Страница 7: ...he DNA loading and staining reagents ROTI Load DNAstain1 2 or 3 containing the non toxic fluorescent dye EvaGreen see below 5 Select your settings accordingly In order to run the system at constant voltage switch the mode button to the Voltage setting and alter the value to the desired setting as described in Set Up Mode the Volt LED will be illuminated by this stage 6 Use the same principle to ru...

Страница 8: ... shielding the unit from light by a dark cloth or paper 2 In order to conserve the blue light lamp the light will be there for 10 seconds only and then switch off again by its own Press the button for 3 seconds to switch constant light on Using runVIEW for DNA recovery 1 Cast a gel featuring two rows of wells with one matching pair of the preparatory combs supplied before transferring the blue lig...

Страница 9: ...oresis the application of current through a gel leads to a build up of heat resulting in the accumulation of condensation within the runVIEW lid viewing pane Excessive levels of condensation impair visualisation of the nucleic acid bands within the gel Condensation may be cleared by using the fan extractor in runVIEW lid G At the End of the Run Turn the power supply settings to zero turn off mains...

Страница 10: ...or ROTIPHORESE PROfessional II electrophoresis unit Special lid for runVIEW system green and red fluorescence 3680 1 Power Supply Roth MINI 2907 1 Power Supply EV1450 CP56 1 Further accessories see Professional II unit Art No 2799 1 K Additional Items and Reagents Levelling table N854 Agaroses Standard 3810 NEEO ultra quality 2267 Agarose Tablets HP67 Broad Range for all fragment lengths T846 GTQ ...

Страница 11: ...suitable for all PROfessional gel trays up to a size of 26 x 32 cm W x L When using gel trays up to a size of 10 x 10 cm 2 gels can be poured simultaneously PROfessional I and II The level bubble allows an optimal levelling of the gels Additional sealing is not necessary L Troubleshooting and Maintenance Each runVIEW system uses all solid state components and should require no maintenance or recal...

Страница 12: ...ircuit is broken in the electrophoresis system Check the connections to the power supply and within electrophoresis system to make sure the connection is intact check the electrodes to make sure they are intact Close the circuit by reconnecting the cables Press START STOP to restart the run High resistance due to tape left on a pre cast gel incorrect buffer concentration or insufficient buffer vol...

Страница 13: ...2020 Complete electrophoresis system ROTIPHORESE PROfessional runVIEW 4849 1 Electrophoresis Station ROTIPHORESE PROfessional runVIEW Base Suitable for Unit PROfessional III 4850 1 Electrophoresis Station ROTIPHORESE PROfessional runVIEW Base MINI 1 Suitable for Unit PROfessional I 9958 1 Electrophoresis Station ROTIPHORESE PROfessional runVIEW Base MINI 2 Suitable for Unit PROfessional II 9961 1 ...

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