R-Biopharm RIDA ABSORBANCE 96, Руководство пользователя

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RIDA
®
ABSORBANCE 96 Version 1.0 | Bedienungsanleitung/User manual
39
2 Instructions for using the RIDA
®
ABSORBANCE 96
2.2 Measurement method
The RIDA
®
ABSORBANCE 96 is designed to carry out fast and sensitive absorbance
measurements. It measures the optical density (OD) of samples at defined wavelengths.
Absorbance/Transmission
Absorbance refers to the amount of light absorbed by a medium. Absorption reduces
transmission. Transmission is the ratio of incident to transmitted light. Accordingly, the degree
of transmission is calculated as follows
T = (I/I
0
)
where I is transmitted light and I
0
is incident light.
Optical Density
Optical density is a measure of the attenuation of light radiation after it has passed through a
medium. Optical density is the logarithmic quantity that describes the reciprocal of the
transmittance T:
OD = log(I
0
/I)
where I is transmitted light and I
0
is incident light.
Optical density is the absorbance of the sample plus other attenuating effects such as
scattered light due to turbidity. It is therefore necessary to avoid scattered light in order to
measure absorbance correctly.
If no other attenuating effects are present, absorbance = optical density.
2.3 About the RIDA
®
ABSORBANCE 96
The RIDA
®
ABSORBANCE 96 is an absorbance measuring instrument, i.e. a measuring
instrument, with which the absorbance values of a 96-well microtiter plate can be read,
recorded and further processed. The RIDA
®
ABSORBANCE 96 has 96 detection units, which
allow measurement without a scanning mechanism.
In the upper part of the measuring instrument are 4 light sources with different wavelengths
(405, 450, 540, 630 nm) and 4 interference filters. The four signal lights on the top of the
reader show, which measuring channel is used for the current measurement.