21
Figure 3. Experimental data obtained for the melting transition of the synthetic alternating
copolymer of deoxyadenosine and deoxythymidine, poly(dA-dT)
using a linear temperature
ramp. Data are shown for the copolymer alone (black) and for the copolymer in the
presence of ethidium (gray). Panel A shows the temperature as a function of time. Panel B
shows the average absorbance as a function of time. Panel C combines the data in A and B
to show the change in the average absorbance as a function of temperature.
Version 01-31-19
34
36
38
40
42
44
46
48
50
52
0
2
4
6
8
10
12
14
16
tim e (m in)
p
ro
b
e
t
e
m
p
e
ra
tu
re
(
d
e
g
C
)
0.34
0.36
0.38
0.4
0.42
0.44
0.46
0.48
0
2
4
6
8
10
12
14
16
tim e (m in)
a
b
s
o
rb
a
n
c
e
(
a
v
g
2
5
5
-2
7
2
)
0
0.02
0.04
0.06
0.08
0.1
0.12
35
40
45
50
tem perature (deg C)
c
h
a
n
g
e
i
n
a
b
s
o
rb
a
n
c
e
(
a
v
g
2
5
5
-2
7
2
)
A
B
C
