3
6.
Q-Blue
CD
with
temperature control software,
help file and this qpod 2e manual.
C. BATH 100
1.
submersible pump
2.
plastic bucket
3.
a length of tubing
D. Optional Items
- If you ordered
any optical
components separately, such as polarizers or
optical filter holders, you will find them packed in a
separate internal box.
SYSTEM SETUP
1.
If desired, fasten the qpod 2e down to a table
or optical breadboard. The holes in the
corners of the base are located and sized such
that it will attach via ¼-20 screws to a table
with 1-inch centers or with M-6 screws to a
table with 25 mm centers.
2.
Supply circulating water to the Peltier unit.
Attach tubing from the submersible pump to
either one of the water hose barbs on the qpod
2e. Attach another piece of tubing from the
qpod 2e back to the bucket. Put water in the
bucket, turn on the pump by plugging it into an
outlet and check to be sure that water is
flowing back into the bucket. Check for leaks.
Unplug the pump until you have completed the
remainder of the system set up.
3.
If you plan to work at low temperatures,
connect a source of dry gas (typically nitrogen)
using a leng
th of tubing with 1/8” (3mm) inside
diameter, to the hose barb labeled “gas” on
the side of the qpod 2e. A flow of gas must be
used to prevent condensation on the faces of
the cuvette when working below the dew point
temperature. Set the dry gas flow rate to 50 -
200 cc/min.
SOFTWARE INSTALLATION
The
qpod 2e
may be controlled using a USB cable.
If you will be using USB to control the
qpod 2e
you
must install the software before connecting the
hardware to the computer, otherwise the
sequence is not important.
4.
Insert the Q-Blue software CD into the drive. If
the installation does not start automatically,
locate the Setup.exe file in the root directory
and run it. The installation process starts with
a small black window that is shown during
installation of the drivers needed to control the
qpod 2e
through a USB connection. This
window will then be replaced by the software
installation window. Follow the onscreen
prompts to complete the installation.
Setting up for temperature control using USB
5.
Connect the
qpod 2e
to your computer using
the USB cable provided. The New Hardware
installation process will begin automatically
and take a few moments to finish.
OPTICS
There are two basic optical configurations for the
qpod 2e
: one to measure
absorbance
and the
other to measure
fluorescence
.
Optics to measure absorption
Figure 1
- Absorbance configuration
As shown in
Figure 1
, to set the
qpod 2e
up for
absorbance measurements insert collimating lens
