Qiagen PyroMark Q24 MDx, Руководство пользователя

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Operating Procedures
PyroMark Q24 MDx User Manual 01/2016 5-5
Optional
If desired, enter a note about the assay and set up the
variable positions in the “Variable Positions” tab (AQ and
CpG assays only).
If creating a CpG assay, it is recommended that bisulfite
treatment controls are added. In the sequence before
bisulfite treatment, check if the suggested bisulfite controls
are Cs converted to
T s (read as Gs and A s in a reverse
assay) and are suitable as controls, or not.
To add a control, left-click a bold, orange
T or A in the
histogram, preferably at the beginning of the sequence.
5.2.3 Setting up a run
1. In the shortcut browser, right-click the folder you want to
place the run file in and select “New Run” from the
context menu.
Note : To add a shortcut to a folder in the shortcut
browser, click “Add Folder Shortcut”.
2. Enter the file name and press “Enter”.
3. Select “Instrument Method”; see Section 5.2.4 for
detailed instructions.
4. Add an assay to each well used, e.g., drag an assay
from the shortcut browser to a well or a selection of
wells.
A well is colored according to the assay loaded into the
well.