I.B. Maxwell
®
16 Blood DNA Purification Procedure
The Maxwell
®
16 Clinical Instrument provides automated nucleic acid purification from up to 16
samples using cell lysis and binding of magnetized silica particles to nucleic acid as the primary
separation principle. Used in conjunction with the Maxwell
®
16 Clinical Instrument, the Maxwell
®
16
Blood DNA Purification Kit (Cat.# AS1015) provides high purity DNA extraction from blood and buffy
coat samples. The purified DNA is eluted in a 300µl volume.
The automated steps performed by the Maxwell
®
16 System include:
•
Sample lysis in the presence of a chaotropic agent and detergent
•
Binding of nucleic acids to magnetized silica particles
•
Washing of the bound particles away from other cellular components
•
Elution of nucleic acids into a formulation that can be added directly to standard PCR.
The user selects the clinical protocol as prompted by the Maxwell
®
16 Instrument, places samples into
the reagent cartridges, places the cartridges onto the instrument platform and closes the door. The user
then starts the instrument, which automatically performs all the steps in the protocol, allowing the user
to walk away and do other work.
The temperature of samples is regulated by a heating system that is controlled by the protocol.
The extracted nucleic acid can be used for PCR amplification.
Promega Corporation ·
2800 Woods Hollow Road
·
Madison, WI 53711-5399 USA
·
Toll Free in USA 800-356-9526
·
Phone 608-274-4330
·
Fax 608-277-2516
· www.promega.com
Part# TM300
Printed in USA.
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