15
Connection of CO
2
gas cylinder
WARNING
Check the gas type and ensure that it is fit for the purpose. Make sure that all pipes are
connected correctly and are not liable to become disconnected. Ensure that the gas pressure is
set at the specified value. Improper connection of the gas pipe or use of incorrect gas pressure
may result in leakage of CO
2
gas.
Elevated level of CO
2
gas can be hazardous to health and may
lead to asphyxiation and risk of death.
Use a liquefied CO
2
gas cylinder, not a siphon (dip tube) type. The CO
2
gas should be 99.5 % or more
pure.
1.
Use a gas regulator rated at 25 MPa(G) (250 kgf/cm
2
(G), 3600 psi(G)) on the primary side and 0.2
MPa(G) (2.0 kgf/cm
2
(G), 30 psi(G)) on the secondary side with flow rate of 77 L/min.
2.
Using the gas supply pipe provided, connect the gas regulator to the connecting port for CO
2
gas pipe
located at the rear upper side of the CO
2
incubator.
3.
Set the CO
2
pressure on the secondary side to 0.1 MPa(G) (1.0 kgf/cm
2
(G), 14.5 psi(G)) (at gas
injection). Excessive pressure may cause disconnection of internal pipes inside the CO
2
incubator which
will result in leakage of CO
2
gas into the atmosphere.
Elevated level of CO
2
gas can be hazardous to
health and may lead to asphyxiation and risk of death.
The repair of the incubator will be necessary
if the internal pipe is disconnected.
4.
Check that no gas is leaking at any point where the pipe connects with the gas regulator or the CO
2
incubator.
Note:
• Refer to “Procedure for replacement of gas cylinder” enclosed with the unit at the time of replacement.
• The incubator, including the gas supply pipes and services must be examined at frequent intervals to
ensure they are safe. Ensure that items such as pipes are replaced if there is any sign of deterioration.
