20
INSTALLATION
Connecting a CO
2
gas cylinder
Before connecting a CO
2
gas cylinder, read the precautions for safe operation on pages 5–9.
1.
Get a CO
2
gas cylinder ready and install an optional gas regulator MCO-010R.
Notes:
• Use a liquefied-CO
2
gas cylinder (at least 99.5 % pure). The siphon (dip tube) type cannot be used.
• When MCO-010R is not available, install a gas regulator rated at 25 MPa(G) (250 kgf/cm
2
(G),
3600 psi(G)) for the primary side, and 0.25 MPa(G) (2.5 kgf/cm
2
(G), 36psi(G)) for the secondary side.
2.
Using the gas tube provided, connect the
connecting port A for the CO
2
gas pipe and the gas
regulator of the CO
2
gas cylinder.
Notes:
•
By using two tube bands provided, connect the gas tube tightly to prevent it from coming off.
•
Make sure that the gas tube is not folded.
•
If the CO
2
gas is supplied to multiple CO
2
incubators from a single gas cylinder, a CO
2
solid will be formed
in the gas regulator. The gas regulator safety valve will operate, and there may be an explosive sound.
3.
After connecting the gas tube, make sure that no gas is leaking (ex. by using a gas leak detector spray).
4.
Set the CO
2
gas on the secondary side to 0.03 MPa(G)–0.1 MPa(G) (0.3 kgf/cm
2
(G)–1 kgf/cm
2
(G),
4.4 psi(G)–14.5 psi(G)) for gas injection. Recommended pressure: 0.03 MPa(G) (0.3 kgf/cm
2
(G), 4.4
psi(G)).
Note:
As the pressure increases, the CO
2
gas density variation will increase. Excessive pressure may
cause gas supply lines inside the incubator to come loose, which may result in gas poisoning or oxygen
deprivation due to leaking gas. If gas lines come loose, the incubator must be repaired.
5.
When there is no CO
2
gas left and the CO
2
gas empty alarm is activated, replace the empty gas cylinder.
Note:
When the optional gas auto-changer MCO-21GC is installed, it switches the empty CO
2
gas supply
line to the other automatically. Refer to pages 59–60.
Note:
The gas lines connected to the incubator will degrade over time. If any deterioration or abnormalities
are found during inspection, replace the lines immediately.
Connecting port A for CO
2
gas pipe
Lower right side