PerkinElmer RamanMicro 200 Series, Руководство по началу работы

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84 . RamanMicro 200 Series Getting Started Guide
Intensity calibration should typically be performed on an annual basis – that is, during an
annual instrument service – but also whenever the instrument is moved, following a service
repair or when there has been significant temperature variation in the local environment.
NOTE: As intensity calibration is not required on a regular basis, NIST Standards are not
included in the basic RamanMicro 200 package. Standard Reference Materials can be
purchased from PerkinElmer (L1321831). Your instrument will be intensity calibrated
prior to shipping and during installation.
NOTE: The intensity calibration should be performed for each sampling accessory. In the
case of the RamanMicro 300 Accessory, an intensity calibration is required for each
objective lens.
1. Ensure that the RamanMicro 200 is switched on.
If not follow SOP-1.
2. Select the 50x objective lens on the microscope.
3. Place the NIST standard in a suitable sample holder.
4. Select
Raman Instrument
from the Setup menu.
The Setup Instrument tabs are displayed.
5. Select
Raman Instrument
from the Setup menu.
The Setup Instrument tabs are displayed.
6. On the Setup Instrument Basic tab select
Microscope
or
Microscope XYZ Stage
as
appropriate from the
Accessory
drop-down list
7. Select
Microscope 50x
from the Objective drop-down list.
8. Select the appropriate
Sample Holder
in the drop-down list and load the sample holder
onto the sample stage, following the instructions on-screen if you have a motorized
stage.
9. Select the Dark Field (DF) switch position on the microscope and switch off the
illumination.
10. Cover the microscope with a dark cloth or, if fitted, close the light-proof enclosure doors
and ensure that caps are placed on any external fiber optic probes or accessories, to
minimize stray light from interfering with the calibration.
11. Select the Setup Instrument Advanced tab, and ensure that the
Laser Power
is set to
100%.
12. Focus on the appropriate NIST standard with the dull surface facing upwards.
If you have a motorized stage you can use the joystick or Stage Position controls on the
SetupMicroscope XYZ Stage tab. Alternatively, click
Raman AutoFocus
to focus
using the strongest Raman signal. The Focus Limits should be set to
Full Range
.
If you do not have a motorized stage, you may find it useful to Monitor your sample to
view the Raman signal while manually adjusting the focus.
13. When the polystyrene sample is optimally focused for Raman signal, select
Toolbox
on
the Setup Instrument Advanced tab.
The Adjustments Toolbox dialog is displayed.