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PerkinElmer 200 UV/VIS Series, Руководство пользователя

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Using Method Mode

Viewing the Chromatogram

4  - 20

Viewing the Chromatogram

Press the View key to display the detector baseline prior to a run, or to display the chromato-
gram after the detector has been placed into run. The default scale for the Y axis is 1.0 
AUFS. For the X axis, the time is 20 minutes when the detector is in the Ready state. At run, 
the X axis displays the chromatogram for the total analysis time of the running method.

The up/down arrows decrease/increase the Y scale by a factor of two over the range 1.0 to 
0.001 AUFS. 

The following procedure describes how to view the chromatogram graphically.

MAIN METHOD SCREEN

To view the chromatogram, press F1 

 from the Main Method Screen. The View 

Screen, similar to the one below, appears. 

VIEW

1

F1

When you are finished viewing the chromatogram, press the 

 key to return to the 

current method.

return

2

VIEW SCREEN

Содержание 200 UV/VIS Series

Страница 1: ...Series 200 UV VIS Detector User s Manual...

Страница 2: ...tial damages in connection with furnishing performance or use of this material NOTE TotalChrom is the updated version of the software previously marketed as Turbochrom This product is compatible with...

Страница 3: ...vely as industrial commercial or medical test equipment It is exempt from the technical standards specified in Part 15 of the FCC Rules and Regulations based on Section 15 103 c Europe All information...

Страница 4: ...and should be read before proceeding to the installation section Symbols and Conventions Used in this Manual The following graphic symbols and graphic text formats are used in this manual to set apart...

Страница 5: ...that could cause per sonal injury if precautions are not followed This can be any type of hazard When you see this symbol refer to the safety pages in the manual for further information Consulter les...

Страница 6: ...ing conductor disconnected D brancher le cordon d alimentation avant d ouvrir da continuit des masses est essentielle Pour un fonctionnement sans danger Ne jamais uti liser l quipment si le fil de ter...

Страница 7: ...e explains this procedure The following illustration contains safety information for the procedure Figure S 3 Front Panel with door open for a lamp replacement Turn the Detector power switch to the OF...

Страница 8: ...outside the instrument or disconnection of the protective ground terminal may impair the protection provided by the instrument Do not operate the instrument with any covers or parts removed Do not at...

Страница 9: ...ircuit the fuse holder The instrument is to be positioned in a clean area free of dust smoke vibration and cor rosive fumes out of direct sunlight and away from heating or cooling units or ducts The i...

Страница 10: ...s The Series 200 UV VIS Detector was designed for indoor use and will function correctly under the following ambient conditions Temperature 10 35 C Relative Humidity 20 80 noncondensing Altitude 0 200...

Страница 11: ...an cause eye injury wear suitable protective goggles when changing or adjusting the lamp If the detector has been operated recently the lamp assembly will be very hot Check carefully before touching t...

Страница 12: ...low 10 to 12 minutes for the monochromator to become completely filled Continue this nitrogen flow while the instrument is in use Purging is particularly important when the instrument is removed from...

Страница 13: ...ent is able to withstand transient overvoltage according to Installation Cate gory II as defined in IEC 1010 1 Pollution Degree This equipment will operate safely in environments that contain noncondu...

Страница 14: ...ory Cell phones beepers and other similar devices operate in a much higher frequency range and do not cause interference Fuses The following fuses are in the power entry module to protect the instrume...

Страница 15: ...he Series 200 UV VIS Detector and its other system compo nents by a PerkinElmer Service Engineer to ensure its operation within published specifications These tests consist of measuring the most impor...

Страница 16: ...the applicable Material Safety Data Sheets MSDS or OSHA ACGIH or COSHH documents Venting for fumes and disposal of waste must be in accordance with all national state and local health and safety regul...

Страница 17: ...an optional solvent cabinet for example the SEC 4 Do not keep buffer solutions in the system when the instrument is not in use since salt may crystallize out Do not use acidic chlorides with pH less...

Страница 18: ...f column s being used Know the relative polarity and miscibility of the solvents being used Solvents with Low Boiling Points Do not use liquids that have a boiling point less than 30 C Buffers Exercis...

Страница 19: ...ile phase from the pump and flush solvent are made of titanium fluoropolymers or glass This makes the Series 200 UV VIS Detector inert to the most common mobile phases that are used for biological ass...

Страница 20: ...re in Table S 3 Table S 3 Auto Ignition Temperatures of Common LC Solvents Do not use carbon disulfide or other solvents which have an auto ignition temperature below 110 C Solvent Auto Ignition Tempe...

Страница 21: ...d attention If you are injecting clean samples and there is no other apparent cause for the pressure build up remove disassemble and clean the flowcell If you are injecting dirty samples the flowcell...

Страница 22: ...VIS Detector weighs only 11 4 kg 25 lbs improper lifting can cause injury to the back Exercise caution when lifting High Voltage The Series 200 UV VIS Detector contains high voltage up to 375 volts B...

Страница 23: ...Safety and Regulatory Information Warnings from the User s Manual S 21...

Страница 24: ...Safety and Regulatory Information Warnings from the User s Manual S 22...

Страница 25: ...Nitrogen Purge S 10 Detector Flowcell S 10 Storage Conditions S 11 Dimensions and Weight S 11 Additional Electrical Considerations and Warnings S 11 Quality Control Good Laboratory Practices S 13 Def...

Страница 26: ...2 Unpacking Your Detector 2 4 Items Included 2 4 Making Electrical Connections 2 5 Checking Electrical Power Requirements 2 5 Set ting the Operating Voltage and Installing the Fuse 2 5 Installing the...

Страница 27: ...ining a Sequence 5 4 Rules for Defining a Sequence 5 4 Identifying the Methods to Include in a Sequence 5 5 Procedure for Defining a Sequence 5 6 Procedure for Defining a Sequence continued 5 7 Settin...

Страница 28: ...8 1 8 Theory of Operation 8 2 Beer s Law 8 2 Optical Design 8 4 Design Considerations 8 6 Application Notes 8 7 Wavelength Selection 8 7 Detector Response Time 8 8 Enabling External Control 9 1 9 Con...

Страница 29: ...information A Description of the Series 200 UV VIS LC Detector The Series 200 UV VIS LC Detector s Features and Benefits The Major Components of the Series 200 UV VIS LC Detector Powering Up the Dete...

Страница 30: ...function keys on a keyboard located on the front panel of the instrument A backlit Liquid Crystal Display LCD located directly above the keyboard shows both status information about the instrument an...

Страница 31: ...NOTES TO REMEMBER WHEN USING THE DETECTOR When using the S200 Detector with Turbochrom the detector must display the top level method or manual screen before attempting to take control from Turbochrom...

Страница 32: ...llowing components Keyboard and Display All instrument functions are accessed using the keyboard Status information is shown on the screens appearing in the display An overview describing how to perfo...

Страница 33: ...in Components of the Series 200 UV VIS LC Detector Getting Started with the Detector 1 5 Figure 1 2 Front Right Side View of UV VIS Detector Input Output Connector Panel Keyboard and Display Access Do...

Страница 34: ...el The rear panel contains the Power Input Module and the serial ports for the UV VIS Detector The power module accepts the AC line cord and contains the system fuses and volt age selector card You ca...

Страница 35: ...s the power switch located on the rear panel of the Detector to the ON position The Copyright and Self Test Screen first appears on the Liquid Crystal Display The display is located directly above the...

Страница 36: ...nnot save the parameters that you set in Manual Mode The set of parameters that appear the first time you start the detector are the default settings The next time you start the detector in Manual Mod...

Страница 37: ...the information on the screens and also to start or stop certain operations on the UV VIS Detector As you move from one screen to another the screen defined function keys change The functions of the v...

Страница 38: ...y is equivalent to the right cur sor arrow key and operates whenever the right cursor key is operative CE Press CE to clear a typed value and redisplay the original value Detector Control Keys reset P...

Страница 39: ...rement the steps following it by one for example when adding methods in a sequence delete Press to delete the current step and decrement the steps following it by one Use the cursor arrow keys to acce...

Страница 40: ...Started with the Detector Using the Keyboard and Display 1 12 Screen Hierarchy Diagram The following figure provides an overview of the hierarchy of the detector s screens Figure 1 5 Screen Hierarchy...

Страница 41: ...s an overview of how to perform the following basic operations using the LC Detector keyboard Selecting screen options Selecting data fields and entering numeric information Entering non numeric infor...

Страница 42: ...creen corresponding to the selected option appears Typical examples include selecting options from the top level Main Screen and the Configure Screen as shown here F1 F8 To F1 CONFIGURE SCREEN F2 F3 F...

Страница 43: ...press the Enter key For example on the Method Edit Screen shown below the data field for the TIME is highlighted To accept the current entry and move to and highlight the Wavelength field press the ri...

Страница 44: ...es a non numeric entry the options for that field appear as screen defined soft keys To change the current entry in the highlighted field press the corresponding function key F1 through F8 For example...

Страница 45: ...down The caret indicates the selected line The following example shows the three most common types of screen lists the Directory Screen Sequence Screen and the Timed Events Screen caret DIRECTORY SCR...

Страница 46: ...yboard and Display 1 18 Responding to Screen Prompts To respond to a Y N prompt appearing in the Message line of a screen such as the one shown in the following screen press the numeric key to select...

Страница 47: ...ns When you finish making changes to a screen press the key to save those changes Changes you make to a method screen will remain in effect only until you turn off the power to the Detector To permane...

Страница 48: ...glass envelope located in flowcell drip pan Display Graphic LCD module 240 X 64 pixel Series 200 standard Noise filter Digitally controlled active filter with response RSP or peak width PW selectable...

Страница 49: ...ls that the unit is ready to run TE1 and TE2 programmable outputs Control inputs EXT RUN same as pressing the run front panel key EXT STOP same as pressing the reset front panel key AZ same as pressin...

Страница 50: ...Getting Started with the Detector Instrument Specifications 1 22...

Страница 51: ...bes how to install your Series 200 UV VIS LC Detector It includes the following information Preparing your laboratory Unpacking your detector Making the electrical connections Making plumbing connecti...

Страница 52: ...onstant relative humidity between 20 and 80 without condensation Use care when working with hazardous solvents or solvents that produce hazardous by products Adequate Bench Space Provide bench or tabl...

Страница 53: ...tor between the detector and the AC outlet Solvents Mobile Phase Use only HPLC grade solvents in all analyses HPLC grade water and methanol 1 liter each are required for performance verification Waste...

Страница 54: ...arts from PerkinElmer s catalog service as described on the Customer Service page of this manual Table 2 1 Start Up Kit P N N292 0140 Part Number Description Quantity 0992 0513 ONE PIECE FINGERTIGHT T...

Страница 55: ...Fuse The power input module located on the rear panel contains an outlet for the AC line cord the voltage selector card and the system fuse s Inside the power input module is a small voltage selector...

Страница 56: ...tage selector card so that it is opposite the voltage selector arrow THIS NUMBER MUST CORRESPOND TO THE VOLTAGE FOR YOUR LOCATION Figure 2 1 shows the voltage selector card settings for both the 120 V...

Страница 57: ...r Figure 2 2 Fuse Location Installing the AC Line Cord 3 Warning Ground circuit continuity is vital for safe operation of equipment Never operate equipment with grounding con ductor disconnected Do no...

Страница 58: ...ord plug into the AC supply Figure 2 3 AC Line Cord Selections North America NEMA 5 15 0998 8986 Europe CEE 7 Schuko 0999 1415 Europe Switzerland 0999 1413 Europe Italy 0999 1422 Old British Standard...

Страница 59: ...Function Location Power Connector Universal male AC line connector for power cord input contains the line fuse and voltage selector Rear Panel Fuses Time delay fuses 5 x 20 mm for protection of the S...

Страница 60: ...ems have a 1 V FS input If the COMP output is set to 1 V AU the data system will have a calibration of 1 AUFS If small 1 AU peaks are to be observed this gives maximum sensitivity Large peaks however...

Страница 61: ...ng this input from an external contact closure starts the method This performs the same action as the Run key You can run a method when READY appears on the top line of the method display EXT STOP Act...

Страница 62: ...Plug the modular connector into the I O panel connector on the Series 200 LC Autosam pler Figure 2 4 shows a typical connection between the Detector and the Series 200 Pump 4 Refer to Figures 2 5 for...

Страница 63: ...Making Input Output Connections Installation 2 13 Figure 2 5 Connecting a Wire to the Modular Connector...

Страница 64: ...Installation Making Input Output Connections 2 14 Figure 2 6 System Connection Diagram Series 200 LC Autosampler Series 200 LC Pump Series 200 UV VIS Detector and a Data System...

Страница 65: ...used since there will normally be minimal backpressure on the outlet side of the cell Waste tubing PN 0250 6495 is included in the Start up Kit The Start up Kit contains a supply of Fingertight PEEK...

Страница 66: ...Installation Making Tubing Connections 2 16 Figure 2 7 Cutting the 010 inch Tubing Blade Edge or Triangular File Scribe Line Smooth Jaw Pliers...

Страница 67: ...peat the steps above Connecting the Column Outlet Tubing to the Detector 1 Stop the pump 2 Slide the Fingertight connector over the end of the tubing 3 Insert the end of the tubing into the inlet conn...

Страница 68: ...and Their Functions Connection Function Sample In Connector Bulkhead union 1 l6 inch OD 0 010 inch ID for connection of column outlet tubing to heat exchanger inlet Sample Out Connector Bulkhead unio...

Страница 69: ...1 16 inch tubing for a zero dead volume union This type of union makes it easy for you to disconnect the Autosampler from the pump Scavanger Column PerkinElmer Scavanger Column Kit Part No 0258 0204...

Страница 70: ...tion Making Tubing Connections 2 20 Figure 2 8 provides an overview of typical tubing connections between the Detector and other instruments making up an LC system Figure 2 9 Typical System Tubing Con...

Страница 71: ...w to use the detector s Manual mode of operation It includes the following topics Understanding Manual mode Viewing the Detector Manual Settings Screen Setting control parameters Scanning the chromato...

Страница 72: ...de the wavelength range response time or peak width value and timed events You cannot save the parameters that you set in Manual mode The set of parameters that appear the first time you start the det...

Страница 73: ...o manual or method operation press the CNFG softkey The Detector displays the Configure menu shown below To switch to manual operation from the Configure menu press the MAN softkey The Detec tor displ...

Страница 74: ...nse Time RSP You select either PW or RSP when you configure the detector For more information see Chapter 6 Configuring the Detector Once you select PW or RSP the detector displays the available value...

Страница 75: ...f the Screen Description 6 CNFG Softkey Use to display the Configure menu options For more information on configuring the detector see Chapter 6 Configuring the Detector 7 SCAN Softkey Use to perform...

Страница 76: ...tings Screen For more information see Viewing the Detector Manual Settings Screen topic earlier in this chapter The wavelength field is highlighted 1 Use the numeric keypad to enter the wavelength in...

Страница 77: ...in Chapter 8 Principles Press the right arrow key or the key to set the RSP and move to the TE1 field enter enter 4 The relay event may be either O C or M These letters indicate the following O Open...

Страница 78: ...he Scan command tells the detector to scan across all wavelengths in the sample cell To best correlate the scan wavelength you can have the recorder mark the output at a specified interval F8 DETECTOR...

Страница 79: ...ments For high resolution use a slow speed Range For the recorder output enter the desired range of the scan Value range 0 0005 to 3 00 Absorbance Units Full Scale AUFS For increased sensitivity use a...

Страница 80: ...can press F8 The Detector displays the SCAN IN PROGRESS Screen STRT 3 When the scan is finished the detector turns off the recorder if controlled from TE2 and returns you to the Scan screen 4 Press th...

Страница 81: ...VIS LC Detector It includes the following information Understanding Methods Viewing the Method Screen Editing the Current Method Creating a New Method Storing a Method Recalling a Method from the Dire...

Страница 82: ...ring a chromatogram Once you store a method you can recall it and run it You can run an active method The active method is the method that is currently running on the Series 200 UV VIS LC Detec tor or...

Страница 83: ...Method Operation Screen To set the Detector to Method operation press the CNFG softkey The Detector displays the Configure menu shown below CONFIGURE MENU F6 To switch from manual to method operation...

Страница 84: ...nged This remains displayed until you store the method This field is blank when you are creating a new method 4 Detector Status One of the following appears NTRDY Indicates Not Ready This status appea...

Страница 85: ...ent Method topic later in this chapter 7 Elapsed Time Indicates the time in minutes that has elapsed since the start of a run 8 Softkey Labels Displays the options available on this screen Softkey Des...

Страница 86: ...rameters and timed events The control parameters allow you set values for the method such as a wavelength value and peak width value The timed events allow you to control the relay contact closures wh...

Страница 87: ...onse Time topic in Chap ter 8 Principles The parameter can be entered in either Peak Width PW or Response Time RSP You select either PW or RSP when you configure the detector For more information see...

Страница 88: ...splays the Steps in the method The caret highlights Step 1 EDIT 1 You can use the cursor arrow keys or the key to move from field to field In the TIME field enter a time value such as 40 00 to indicat...

Страница 89: ...parameters for Step 1 you can press the key to go back to the Method Screen or you can add another Step to the method return 6 To add another Step to the method press the key or the right arrow key T...

Страница 90: ...To edit the timed events follow the procedure below From the Method Screen press F2 to display the Method Edit Screen EDIT 1 Press F5 to move to the Timed Events Screen The data field for the Time is...

Страница 91: ...on Momentary closures should be preceded with an Open event enter 5 To add another event press either the key or the key A second event will be added and the Time field for the next event will increme...

Страница 92: ...reate a new method press the softkey The detector displays a one step method with the default operating parameters NEW 2 METHOD EDIT SCREEN 3 Use the cursor arrow keys or the key to move from field to...

Страница 93: ...Once you store the method the method status switches from CHNGD changed to STORD stored The STORD status indicates the method is stored in memory When you store a method the method gets added to a li...

Страница 94: ...hat appears corresponds to the next available method number 1 20 Press the key to accept the method number shown or use the numeric keys to type in a new value and then press enter enter 1 2 3 SAVE ME...

Страница 95: ...ry lists the stored methods and shows you the method number status name and date and time the method was last modified Once you recall a method it becomes the current active method To recall a method...

Страница 96: ...c key to make the selected method the active method The detector displays the selected method on the Method Screen RCL yes 1 F6 MAIN SCREEN Press F6 from the Main Screen to go to the Directory Screen...

Страница 97: ...Directory to recall a stored method or to edit a copy of the current method and save it as a new method When you edit a stored method the changes are stored with the method The detector does not physi...

Страница 98: ...making changes to the control parameters For more information see the Editing the Current Method section earlier in this chapter EDIT 3 4 Message line DIRECTORY SCREEN caret METHOD EDIT SCREEN To sto...

Страница 99: ...follow the procedure below run reset METHOD SCREEN To run the current method press the key on the keypad run The detector runs the method and shows time passing in the TIME field The detector runs the...

Страница 100: ...y state At run the X axis displays the chromatogram for the total analysis time of the running method The up down arrows decrease increase the Y scale by a factor of two over the range 1 0 to 0 001 AU...

Страница 101: ...r describes how to use Sequences on your Series 200 UV VIS LC Detector It includes the following information About Sequences Defining a Sequence Running a Method in Sequence Stopping a Sequence Using...

Страница 102: ...atically run a series of methods while the detector is unattended The following are the possible states for a sequence Display Edit The sequence appears on the display and can be edited The sequence s...

Страница 103: ...ps 3 Sequence Status LINKD indicates that the sequence has been set up and is ready to run In the figure above the sequence status is S03 001 The S indicates Sequence The 03 indicates the current set...

Страница 104: ...es for Defining a Sequence If you have not already done so you need to create and store in the Detector s Directory all of the methods that you intend to include in the sequence Procedures for creatin...

Страница 105: ...hods you want to include in the sequence Follow the procedure below to view the methods in the detector s Directory F6 Press F6 from the Main Method Screen to go to the Directory Screen DIR 1 Using th...

Страница 106: ...s for the SET press the right arrow key to move to the INJ field and enter the number The number of injections indicates the number of times to run the method To create another SET press either the ke...

Страница 107: ...rt To delete the entire sequence so that you can create a new sequence press F8 Pressing the key deletes all sets in the sequence To delete a specific step select the step and press the key on the key...

Страница 108: ...injections A linked sequence requires that you press the Run key to run each injection in the sequence A linked method cannot be edited After you press the system asks if you want to turn the lamp off...

Страница 109: ...n key the detector runs the first method in the sequence When the first method has been run for the first injection the detector becomes ready for the next injection or the next method in the sequence...

Страница 110: ...Screen To break or stop a sequence press the softkey Stopping a method also automatically stops or breaks a sequence Softkey Description SET Allows you to select a SET number and review the parameters...

Страница 111: ...ries 200 UV VIS LC Detector It includes the following information Setting Configuration Options Setting up the Instrument Diagnostic Tests for Service Use Validating the Wavelength Calibration Checkin...

Страница 112: ...tions 6 2 Setting Configuration Options Setting the Detector configuration options involves the following tasks which appear on the Configure menu Setting up the Instrument Running Diagnostic Tests Ch...

Страница 113: ...Screen Contrast and Screen Saver Setting the Analog Output Setting the System Clock Adjusting the Smoothing Mode Setting up the Leak Detector You can perform all of these functions from the Instrument...

Страница 114: ...tor Setting Configuration Options 6 4 F7 MAIN SCREEN CONFIGURE SCREEN From the Main Screen press F7 to move to the Configure Screen CNFG Press F1 to move to the Instrument Setup Screen INST 1 2 F1 INS...

Страница 115: ...o move to the desired field From the Instrument Setup Screen press F1 to move to the Lamp Setup Screen The the LIMITS field is highlighted 1 2 3 To accept the entry and move to the next field you can...

Страница 116: ...22 being the most bright SCREEN CONTRAST SCREEN From the Instrument Setup Screen press F2 to move to the Screen Contrast Screen SCRN 1 2 3 To increase the screen contrast press F4 To decrease the scr...

Страница 117: ...to offset the value of the detector output to a positive value to allow for any potential negative drift Computer Output Allows to you to set the number of volts per absorbance unit Recorder Adjust A...

Страница 118: ...ne If the signal drifts below the range it cannot be seen on the recorder To get the signal back into the range you can adjust the offset to a positive value 1 2 Use the cursor arrow key to navigate t...

Страница 119: ...your data handling system to identify the required voltage 1 2 Use the cursor arrow keys to navigate to the COMPUTER OUTPUT field Select one of the following softkeys to change the value in the field...

Страница 120: ...To test the recorder select one of these settings CALB Puts out 10 mv ZERO Sets the recorder output to zero volts 1 2 Use the cursor arrow key to navigate to the RECORDER ADJUST field Select one of th...

Страница 121: ...in DATE 12 becomes highlighted Use the numeric keys to enter the new day To move to the month character field 15 in DATE press F2 and use the numeric keys to enter the new month Set the year and the...

Страница 122: ...Width at Half Height in Seconds Allows you to set the smoothing parameter in terms of how far the peak rises For information on selecting RSP or PW as a control parameter see Chapter 3 Using Man ual M...

Страница 123: ...entry is made in the error log of the appropriate error message CONT The system continues to run No change occurs in the detector s state An error message appears on the display The error message is c...

Страница 124: ...creen LEAK From the Instrument Setup Screen press F8 to move to page two of the Instrument Setup Screen MORE 1 2 3 Use the cursor arrow keys or the key to move from field to field Press the corre spon...

Страница 125: ...lectronic and mechanical performance of the Detector This section describes only how to access these options For more information on detector diagnostics contact your PerkinElmer service representativ...

Страница 126: ...results from the detector When you select CHK from the Configure menu the Detector displays the Wave length Calibration Check Screen This screen allows you to run a check on the wavelength calibration...

Страница 127: ...r the Holmium Oxide Filter and then runs the calibration test STRT 3 When the test is complete the Detector displays the results of the test and instructs you to remove the Holmium Oxide Filter Press...

Страница 128: ...ctronic failure If replacing the lamp does not clear the problem please contact your local PerkinElmer service representative FAN FAILURE The system has detected that the fan is not turning the lamp w...

Страница 129: ...er service representative A Short or Open error on the External Leak Detector may be caused by incorrect installation of the leak detector Check the installation If all appears well then the error is...

Страница 130: ...the lamp hours if necessary for example you can reset the lamp hours when you install a new lamp in the Detector LOG 2 To view the Detector s error log press F8 A screen similar to the following appea...

Страница 131: ...odic maintenance procedures that must be performed to keep the detector in optimal working condition The chapter also provides systematic pro cedures for isolating and repairing problems with the inst...

Страница 132: ...e acceptable The procedure outlined here takes the user through a step by step maintenance check It must be remembered that the Series 200 UV VIS LC Detector is just a single component in a complete H...

Страница 133: ...to keep it in proper working condition Routine maintenance consists of replacing the normal wear items such as the deuterium lamp and columns whenever you notice a degradation in performance The exit...

Страница 134: ...roximately 25 mL of tetrahydrofuran THF through the flowcell via the bulkhead connections THF should be pumped directly into the bulkhead unions from the pump and out to waste Disconnect all other sys...

Страница 135: ...5 Figure 7 1 Flowcell location Top front view of Detector Figure 7 2 Flowcell compartment Front view of Detector Inlet fitting Heat exchanger Flowcell door Outlet fitting Flowcell screw Heat exchange...

Страница 136: ...ws on the exit aperture gradually and uniformly until the aperture pressure on the flowcell windows is removed Remove the screws com pletely Also remove the apertures and the windows from both sides F...

Страница 137: ...lling the Flowcell First reconnect the flowcell to its connecting tubing and the bulkhead bracket The flowcell should now be reinstalled as a complete assembly Before final positioning of the flowcell...

Страница 138: ...periodically cleaned with a soft cotton swab and a solvent such as methanol Cleaning the Photodiode Windows 1 Open the Access door 2 Loosen the knurled nut and swing open the front panel 3 Swing the...

Страница 139: ...flowcell plate 4 Remove the window housings by inserting a large blade screwdriver into the slots in the window housings and turning carefully counter clockwise 5 Clean the windows and allow them to...

Страница 140: ...retaining rings into the housing assembly 2 Place the completed window housing assembly into the flowcell clamp and retighten into the flowcell plate 3 Replace the sample and reference cells and close...

Страница 141: ...ne cord from the AC outlet 2 Allow the detector to cool for 15 30 minutes before proceeding 3 Loosen the knurled nut on the back panel 4 Open the access door Avertissement Ultraviolet radiation from t...

Страница 142: ...mp retainer spring 11 Grip the entire lamp with one hand while holding the detector steady with the other Slide the entire lamp assembly off the lamp mount 12 Unplug the lamp Figure 7 5 Preparing to s...

Страница 143: ...Unfasten the lamp retainer spring 6 Grasp the entire lamp with one hand while holding the detector steady with the other Slide the lamp assembly from the lamp mount Figure 7 6 Locating the lamp Instal...

Страница 144: ...excessive noise or drift Excessive noise and drift after a lamp change may indicate that the lamp is improperly seated Remove the lamp reinstall and recheck If any problems persist call your PerkinElm...

Страница 145: ...the mobile phase between the HPLC pump and column out let because of the relatively high pressures in that area of the chromatographic system However these gases may bubble out of solution after leavi...

Страница 146: ...matter Sonication may also be used to accomplish degassing Place the vessel containing the mixed mobile phase into a sonicator containing about 1 2 inch of water or more depending upon the size of th...

Страница 147: ...tion Cer tain strategies can be used to minimize their negative effects UV Cutoff The UV cutoff of an HPLC grade solvent is usually defined as the wavelength at which the solvent exhibits 1 0 AU absor...

Страница 148: ...Detector is set for the correct voltage 2 Turn on the power You should see the Power Up screen and the lamp should light If nothing happens disconnect the instrument from the power line and check the...

Страница 149: ...ental Whenever a material is warmed or cooled its dimensions change The monochromator of any absorbance detector will follow the thermal changes of its environment with slight changes in its physical...

Страница 150: ...ularly important when an instru ment is removed from a cold room to a high humidity room at ambient temperature Compressed gas cylinders must be safely attached to the wall or bench by means of approv...

Страница 151: ...ak Check waste line for flow then trace tubing for a leak Contaminated mobile phase Replace mobile phase Wavelength setting below the cutoff wavelength of the mobile phase Select the correct wavelengt...

Страница 152: ...vel is not too low Apply back pressure at outlet or connect a backpressure device P N 0990 7126 Air bubbles in the flowcell Degas the mobile phase Connect a backpressure valve P N 0990 7126 Bypass the...

Страница 153: ...e with new column Peaks are broad loss of resolution Column losing efficiency Invert and flush the column If regeneration is unsuccessful replace and equilibrate with column Change in mobile phase Pre...

Страница 154: ...ty flowcell Clean and or rebuild the flowcell High flowcell back pressure Plugged flowcell Clean or rebuild the flowcell Up scale drift higher absorbance levels Chromatographic problems Replace the fl...

Страница 155: ...ents are covered by PerkinElmer for labor travel and service The following basic information should be available when calling the PerkinElmer service group In many cases successful troubleshooting can...

Страница 156: ...Maintenance Troubleshooting Methods 7 26...

Страница 157: ...principles of operation of the Series 200 UV VIS LC Detector and also provides some guidelines on determining appropriate values for key parameters Topics include Theory of Operation Application Notes...

Страница 158: ...that certain solutions absorb light in an amount directly proportional to the concentration of solute presented to the light Bouger and Lambert discovered that when light was passed through a thin lay...

Страница 159: ...n in the cell The linear relationships pre dicted by Beer s Law remain strictly true only for monochromatic single wavelength light beam Also the linear relationship between absorbance and concentrati...

Страница 160: ...The Series 200 UV VIS LC Detector s dual beam optical design consists of either a deute rium for 190 360 nm or tungsten for 360 700 nm light source a patented monochromator a flowcell and reference ce...

Страница 161: ...while maintaining a narrow bandwidth the Series 200 UV VIS LC Detector uses a bandwidth of 5 nm In the PerkinElmer dual beam design the intensities of the light passing through the sam ple flowcell an...

Страница 162: ...and grat ing for wavelength selection Flowcell Flowcell supplied for sample path operation 12 L 8 mm path std Reference Dry Cell Dry cell supplied for reference path operation Stepper Motor Stepper mo...

Страница 163: ...oosing a wavelength off of the absorbance maximum peak can also be used to decrease the molar absorptivity sensitivity for an analysis with concentrated solutes This will help increase the linearity o...

Страница 164: ...imited In order to obtain the best signal to noise ratio S N an elec tronic filter is added to eliminate noise frequencies while allowing all signal frequencies to pass unattenuated However some frequ...

Страница 165: ...ate and pressure variations teaks temperature variations aging lamps and gas in the detector cell Response Time Versus Time Constant The time constant value describes the degree of filtering of freque...

Страница 166: ...baseline will contain much less short term noise However larger rise times may have the following effects on peaks Peak shape will become asymmetric The peak maximum will be shifted The peak height wi...

Страница 167: ...wing information Configuring Turbochrom with the Series 200 UV VIS Detector The Series 200 UV VIS Detector Configured into Turbochrom as a 785 Detector The Series 200 UV VIS Detector Configured into T...

Страница 168: ...nfigured into Turbochrom as a Series 200 UV VIS Detector In this mode the method capabilities of the Series 200 UV VIS Detector are available from Turbochrom enabling the use of wavelength changes dur...

Страница 169: ...pplied to the digital chromatographic signal This offset is automatically removed by Turbo chrom in its chromatographic displays The offset is also present in both recorder and com puter output analog...

Страница 170: ...Enabling External Control Configuring Turbochrom with the Series 200 UV VIS Detector 9 4...

Страница 171: ...Scanning 3 8 Viewing 4 20 Cleaning Monochromator Exit Windows 7 9 Photodiode windows 7 8 Column scavanger 2 19 COMP and REC outputs 2 10 Computer Output 6 7 Adjusting 6 9 Computer Integrator Connecti...

Страница 172: ...al Power providing sufficient 2 2 Electrical Safety Guidelines S 7 Electrical Warnings S 6 Environmental Considerations S 8 Error Log Viewing 6 18 Exposure Ultraviolet Radiation S 20 F F1 through F8 o...

Страница 173: ...aintenance Guidelines 7 3 Making electrical connections 2 5 Making Plumbing Connections 2 15 Manual Mode Setting Operating Parameters in 3 6 Manual mode Switching to 3 3 Using 3 2 Manual Operation 1 8...

Страница 174: ...RSP 4 7 Running a Method 4 19 Running a sequence 5 9 S Safety Electrical S 6 High voltage S 20 Moving the detector S 20 Suitability test S 13 Sample In Connector 2 18 Sample Out Connector 2 18 Satura...

Страница 175: ...Guide 7 21 Tubing Connections Display of 2 20 Tubing connections 2 15 Tubing Connections making 2 15 Tubing Connections making external 2 19 Tubing Preparing to cut 2 15 Tungsten Lamp 6 5 8 6 Turbochr...

Страница 176: ...Index In 6...

Страница 177: ...kinelmer com The most up to date information on part numbers product brochures systems modules spare parts and application notes are in the PerkinElmer website Telephone U S only Call toll free 1 800...

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