14
PALM
®
MicroBeam User Manual - 0202 Z
The PALM
®
MicroBeam - Survey
Microscope Zeiss Axiovert 200
5.5 Microscope Zeiss Axiovert 200
Figure 7
Survey of the Microcope
The high optical and mechanical quality Zeiss
inverse microscope Axiovert 200 is part of the
PALM
®
MicroBeam. This microscope can also be
used as a normal research microscope. Please
observe the Axiovert 200 user´s manual.
The laser beam is guided through the microscope
adapter (see section 5.4, page 13) into the epiflu-
orescence channel of the microscope. It is
reflected by a special coated beam splitter in the
filter revolver and focused by the objective.
It is necessary to observe the following instruc-
tions, otherwise the laserbeam might be blocked
and performing microdissection will not be possi-
ble:
• The electromagnetic fluorescence shutter in the
microscope has to be opened. This can be done
with the button "FL on/off". When the shutter is
open, the sign "FL" appears on the display of
the microscope.
• The filter revolver has to be in the position in
which the beam splitter reflects the laserbeam.
This position is labeled with "Laser". In case of
installed fluorescence attachment (option), it is
possible to use also the installed beam splitters
of the fluorescence filtersets (only in case of
original used P.A.L.M. beam splitters).
• The two diaphragms in the epifluorescence
channel must be open.
• The adjustment aid for the fluorescence illumi-
nation has to be in the left position, otherwise
the laserbeam will be blocked.
• Do not insert any filters in the filterslider in the
epifluorescence channel. They might block the
laser beam.
1
8
5
9
6
4
3
7
1 display
2 special coated beam splitter
3 button for fluorescence shutter
4 fluorescence attachment (option)
5 microscope
adapter
6 epifluorescence
channel
7 adjustment
aid
8 two diafragms
9 filterslider
2
filter
revolver
set to
"Laser"
FL-shutter "open"
1
filters and
diaphragms
"open"
adjustment aid in
"left position"
Notice
If the illumination arm of the microscope is
pushed back, the laser will be switched off
immediately. This is a security device, which
protects you from looking into the laserbeam.