22
PALM
®
MicroBeam User Manual - 0202 Z
PALM
®
MicroBeam with Fluorescence Illumination
Cutting speed
6.6 Cutting speed
The microdissection of big areas usually should be
performed with a low magnification objective at
comparatively high energy values with a high cut-
ting speed. These parameters automatically yield
a broad cutting line.
To reach a fine cutting line it is necessary to opti-
mize the laser energy with regard to the cutting
speed, as the laser pulse repetition rate is fixed at
30 pulses per second.
7 PALM
®
MicroBeam with Flu-
orescence Illumination
7.1 Operate the Microscope with Fluores-
cence Illumination
The epifluorescence equipment is attached with
clamping screws to the nozzle of the illumination
insert at the back of the microscope.
7.1.1
Simultaneous
Fluorescence
Observation
The beam splitter (in the fluorescence slide switch
directly in front of the objective, in the reflector
slide or in the fluorescence cube) is custom-made.
The beam splitter must have a special coating suit-
able for the simultaneous reflection of the laser
light and the fluorescence excitation wavelength
as well as for the transmission of the emitted fluo-
rescence light.
If required, please contact P.A.L.M. Microlaser
Technologies AG.
Notice
Make sure that there is no filter within the
beam path of the laser that could block the
laser transmission.
Therefore, the excitation filter is mounted out-
side the laser beam path directly between the
fluorescence lamp housing and the laser coup-
ling in the filter wheel.
Caution
Do not use at any rate the beam splitters deliv-
ered by microscope manufacturers along with
the filter because such beam splitters would be
destroyed by the high energy of the laser.