18
PALM
®
MicroBeam User Manual - 0202 Z
How to Prepare the PALM
®
MicroBeam for the Application
Switch On/Off the Device
6 How to Prepare the PALM
®
MicroBeam for the Application
6.1 Switch On/Off the Device
6.1.1
Switch On the Device
• Set the key switch of the ControlUnit to ON and
switch on the computer (for details see „PALM
®
ControlUnit“ on page 12).
The PALM
®
RoboSoftware will be loaded auto-
matically. If not, please see User´s Manual Part
III, PALM
®
RoboSoftware how to start it.
6.1.2
Switch Off the Device
• Exit WINDOWS.
• Switch off the ControlUnit.
• Switch off the multiple socket.
6.2 CCD Video Camera Adapter
6.3 Check of the Laser in the Microscope
The laser is coupled into the microscope via the
epifluorescence beam path.
Prior to the check of the laser, make sure
- that the aperture of the iris diaphragm in the
epifluorescence beam path is completely open,
- that there is no filter within the beam path of
the laser,
- that a possibly existing filter slide is set to free
passage,
- that the filter block or slider in front of the
objective is switched into the correct position
called "laser"
- FL on adjustment failed
- that the FL shutter in the microscope is open:
"FL" appears in the display.
- adjustment aid is in left position
Notice
If the red system LED of the control unit
doesn’t turn off automatically, please witch off
the PALM
®
ControlUnit and after 10 seconds
switch it on again. Should the LED not turn off
please contact P.A.L.M. Microlaser Technolo-
gies AG.
Caution
Do not touch the camera! If camera position is
changed, new calibration is necessary (please
refer to the PALM
®
RoboSoftware Manual how
to calibrate your system.
Notice
Depending of the Camera Adapter, the com-
puter monitor only shows a section of the area
visible in the ocular.
Caution
Only use the objectives labelled with a PALM
logo for the laser operation.
Objectives designed for oil immersion or water
immersion must be used with the respective
immersion medium, otherwise the objectives
might be damaged.
Caution
- Do not turn the objective revolver during
simultaneous laser operation.
- Never use the laser without an objective in
the appropriate position.
- Never insert reflecting objects in the laser
beam path.