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The Otter

TM

Sequencing Gel Casters

Model SGC-1 and SGC-2

O

wner’s

Manual

Rev. Date: 3/2003

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Содержание The Otter SGC-1

Страница 1: ...The OtterTM Sequencing Gel Casters Model SGC 1 and SGC 2 Owner s Manual Rev Date 3 2003 ...

Страница 2: ...mation In order to benefit from the use of this apparatus you must carefully read and understand the contents of this manual prior to use of this apparatus Statement of Proper Use Use this product only for its intended purpose as described in this manual Do not use this product if the power leads are damaged or if any of its surfaces are cracked ...

Страница 3: ......

Страница 4: ...16 ...

Страница 5: ...hecking Your Order 2 Section 2 Using the System 5 Setting Up 5 Gel Casting 6 Operating 7 Section 3 Technical Tips 9 Section 4 Troubleshooting 11 Section 5 Care Cleaning 13 A Few Tips About Caring for Your System 13 Care of Acrylic 14 Section 6 Optional Equipment 15 1 Table of Contents ...

Страница 6: ...orizontal sliding plate procedure capillary action draws the gel between plates Surface tension not tape keeps the gel from leaking No taping or special assembly is required The sliding casting method has been shown to require significantly less time to cast and the clean process decreases exposure to acrylamide and reduces clean up time Gels can be cast in less than one minute Any standard size s...

Страница 7: ...General Information SECTION 1 3 Figure 1 1 Parts Diagram SGC 1 SGC 2 1 Adjustable casting platform 2 knobs for width adjustment attached 12 Binder clamps ...

Страница 8: ...4 ...

Страница 9: ... One or more pairs of glass plates to fit any standard manual or automatic sequencing electrophoresis system Matching spacer set and comb for each gel to be cast Binder clamps 9 per gel cassette Owl catalog CL 12 Rain X or Sigmacote Sigma Chemical St Louis MO Ethanol for washing plates combs and spacers ...

Страница 10: ... must be given during each step of sequencing gel preparation for consistent results Begin by cleaning plates thoroughly 1 To completely clean glass plates of any acrylamide residue left over detergents and dust wash plates with warm water and deter gent 2 Rinse plates completely with deionized water then wipe with 95 ethanol to eliminate any traces of detergent and water marks A final wipe should...

Страница 11: ...sting area is level place the blank longer glass plate into the caster pushing it to the end with the stops The rail it is sitting on is slightly lower than the rail on the other end of the unit 3 Moisten spacers with drops of water and place them flush to the edge of the glass against the side of the rail Moistening helps to keep the spacers from moving while sliding the notched plate over them d...

Страница 12: ...ed and then proceed for ward again until the gel is completly cast 6 Clamp along the spacers in the spaces provided by the caster Clamp in the middle of the spacer and not in the gel area 7 Insert comb see page 9 for Sharktooth and Well Comb instru tions at the top of the gel and clamp with two or three clamps as space allows Clamp in the middle of the comb and not on the teeth or beyond the comb ...

Страница 13: ...vent that the material has any slight variation in thickness Place binder clamps over the comb and glass plates along the upper edge to force the glass plates against the comb This will ensure a tight fit of the comb during and after polymerization If top binder clamps are not used the comb may not be tight enough to prevent sample leaks between wells following gel polymerization Do not clamp the ...

Страница 14: ... usually about 2 cm shorter than the front or blank glass plate Instead of glass ears pieces of adhesive sponge are adhered either to the top of the spacers or to the apparatus itself These sponge tips wear out and may leak Notched glass plates are more fragile and expen sive than offset glass and sponge tips but many researchers prefer notched glass because it is easier to use The third spacer Th...

Страница 15: ...wl catalog B LP If gel will not stay between the plates at the bottom of the cassette this may be caused by bowed plates Try another set of glass or flip the top piece so that the other side is facing the gel solution Dirty glass can also cause slight leaking See cleaning instructions above and in the Gel Casting section of this manual Problem When loading samples the samples seem to be running in...

Страница 16: ...12 ...

Страница 17: ...ination use RNase Away Spray wipe or soak labware with RNase Away then wipe or rinse the surface clean it instantly eliminates RNase RNase Away eliminates the old methods that include treatment with 0 1 Diethyl Pyrocarbonate DEPC treated water and soaking in dilute bleach DEPC is suspected to be a carcinogen and should be handled with care This electrophoresis system should never be autoclaved bak...

Страница 18: ...S Acetic acid Glacial D Ethyl alcohol 95 U Nitric acid 10 S Acetic Anhydride A Ethylene dichloride D Nitric acid 40 A Acetone D Ethylene glycol S Nitric acid concentrate U Ammonia S 2 Ethylhexyl Sebacate S Oleic acid S Ammonium Chloride saturated S Formaldehyde 40 S Olive oil S Ammonium Hydroxide 10 S Gasoline regular leaded S Phenol 5 solution U Hydroxide 10 S Glycerine Heptane commercial grade S...

Страница 19: ...tched Glass S1S 45R Blank Glass S1S 45G 20cmW x 43cmL x 3 16 T Offset Glass S1S 43G 35cmW x 45cmL x 3 16 T Notched Glass S2S 45R Blank Glass S2S 45G 35cmW x 43cmL x 3 16 T Offset Glass S2S 43G 35cmW x 65cmL x 3 16 T Notched Glass S2SL 65R Blank Glass S2SL 65G 35cmW x 63cmL x 3 16 T Offset Glass S2SL 63G Spacers set includes 2 side and 1 bottom Catalog No 45cmL 1 0cmW x 0 4mm thick S2S SA4 1 0cmW x...

Страница 20: ... speed up your return by saving all packing materials cartons and documents until you have thoroughly inspected your shipment Should you find that your order is incorrect or damaged verify the problem with the shipper save all packing mate rial and call Owl for return instructions within 48 hours All returns exchanges and credits must be pre approved by Owl 55 Heritage Avenue Portsmouth NH 03801 T...

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