40
e
、
Resolution
problems
⚫
The image is
not obvious
；
⚫
Contrast to
the bad
；
⚫
Details are
not clear
；
⚫
Not obtain
phase contrast
effect
。
Objective nosepiece is not
turned into optical path
correctly
Be sure that the objective nosepiece
is in the right location.
Aperture is too big or to
Small in bright field
observation
Adjust the aperture correctly
Lens(condenser,objectives,e
yepieces or petri dish) are
dirty
Wipe all
The thickness of the bottom
of petri dish is beyond
1.2mm in phase contrast
observation.
Change to a petri dish the thickness
of whose bottom is not above
1.2mm
Use the bright field
objective lens
Please use phase contrast objective.
The halo of condenser is not
Matched with that of
objective
Adjust the halo to make them
matched with Each other
Halo and phase contrast are
not centering
Adjust the screw to center
The objective is not
compatible with the phase
contrast observation
Please use the objective suitable for
phase
contrast observation
When observing the edge of
petri dish,the phase contrast
ring and halo are
separated
Move petri dish until obtain phase
contrast effect
f
、
Inaccurate
focusing occurs
when using high
magnification
objectives
Objective nosepiece is not
Turned into optical path
correctly
Be sure that thee nosepiece is in the
specified location
Sample is not fixed on the
specimen preparation plate
correctly
Put the sample on the specimen
preparation plate correctly
Optical performances of
petri dish(outline
irregularly,etc) are bad
Please use the petri dish with
regular outline and high quality.
g
、
The focus on
one side of the
field of view (up,
down, left and
right) is not
accurate. Image
flow (i.e.
asymmetric
defocus occurs
when the focus is
moved)
Specimen tilt
Make sure the specimen is tilted
Tilt of transmission lighting
support
Confirm that the lighting support is
in place
The objective lens does not
enter the optical path or is
not installed in place
Ensure that the objective lens
enters the optical path and the
objective lens thread is installed in
place
