3
Superior S/N ratio delivers imaging excellence
UIS2 objectives' fluorescence S/N ratio is improved by using
selected low fluorescence glass material, and minimized
autofluorescence obtained by anti-reflection coating and cementing
material. Also numerical aperture (N.A.) has improved in addition to
the reduction of autofluorescence. Weak fluorescence emissions
are efficiently detected even from weak excitation light, which is
friendly for the living cell.
The ideal fluorescence imaging of live cells with the UIS2 systems!
High transmittance UPLSAPO100XO
T
ransmittance(%)
Wavelength (nm)
0
300
400
500
600
700
800
10
20
30
40
50
60
70
80
90
100
(new) UPLSAPO100XO
(conventional) PLAPO100XO
Focus (
µ
m)
Wavelength (nm)
Comparing chromatic aberration compensation levels:
(The smaller the figure the better)
UPLSAPO series chromatic aberration compensation
-0.5
0
400
450
500
550
600
650
700
750
800
850
900
0.5
1
1.5
2
(new) UPLSAPO100XO
(conventional) UPLAPO100XO
Alexa Fluor488
Cy7
UIS2 optics are designed to maximize S/N ratio and optical
performance for live cell fluorescence imaging.
High transmittances over a wider
wavelength range
UIS2 objective for the IX71/81 achieves flat, high transmittance from
visible to near-infrared light, thanks to new UW multi-coating which
effectively cuts reflection over the super wide band spectrum. In
particular, transmission in the near infrared range is significantly
enhanced. Overall, performance all across the wavelength range is
ideally suited for today's most demanding research applications.
High N.A. objectives for fluorescence imaging
PLANAPO60XO and UPLSAPO100XO have outstanding N.A., 1.42
and 1.4 respectively, suitable for fluorescence imaging and bright-
field observation as well. In addition to their high fluorescence S/N
ratio, both these lenses are able to handle UV excitation light. The
UPLSAPO100XO provides a transmission of down to 340 nm.
Aspherical collector lens provides
excellent excitation efficiency.
Unique, optional ring slit
illumination reduces
objective autofluorescence.
Objectives providing
the best fluorescence
S/N ratios.
High performance mirror units
optimized for fluorescence.
Stray light reduction to
absorb spurious reflections
from dichromatic mirror.
4
Effective compensation for
chromatic aberration up to near-infrared
The UPLSAPO series is the highest class UIS2 objectives, whose
super apochromatic features effectively compensate for chromatic
aberration from the visible spectrum all the way to near IR. This
means that just one objective covers imaging from UV to IR range.
The series also offers outstanding image clarity without color shift
for multi-color observations using fluorochromes covering a wide
wavelength spectrum.
UPLSAPO100XO
Cy7
Wavelength:
767 nm
Alexa
Fluor488
Wavelength:
519 nm
UPLAPO100XO
Objective:
UPLSAPO100XO
Mirror unit:
U-MNIBA3
Objective:
UPLAPO100XO
Mirror unit:
U-MNIBA2
