Confocal laser scanning microscope/
FLUOVIEW FV1000 system
The Fluoview/FV1000 is a next-generation imaging system
designed for high-resolution, confocal observation of both fixed and
live cells. The FV1000 offers advances in confocal system
performance while providing the speed and sensitivity required for
live cell imaging with minimal risk of damage to living specimens.
In addition, the FV1000 offers a revolutionary synchronized laser
scanning system called the SIM Scanner.
While one laser stimulates, the second laser
simultaneously provides high resolution
imaging. This coordination of laser
stimulation and imaging makes the FV1000
an ideal choice for FRAP,
FLIP and photoactivation.
* FV1000 is a class 3B laser product.
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Manipulator
Manipulating cells.
Micromanipulation system/ON3
Olympus' original micromanipulator offers high stability and
excellent stability because of its compact body. Motorized coarse
and oil hydraulic fine three axes operation are designed in its
compact and rigid body with hanging down ergonomic joystick
control. ON3 micromanipulator is securely fixed through the screw
holes on IX2 microscope frame.
ON3-99D
ON3-99D with return mechanism (UT-R)
Manual combination
(ONM-2D+ONO-301D+UT-D)
Manual combination with return mechanism
(ONM-2D+ONO-301D+UT-D+UT-R)
Human embryo
Images of Kaede-expressed cells demonstrating
the photoactivation acquired every 300 msec and
observed via 405 blue diode laser illumination with
twin scanners.
TIRFM
(Total Internal Reflection Fluorescence
Microscopy)
Since 1997, Olympus has been a market leader in objective based
Total Internal Reflection microscope that allows an evanescent
wave illumination approximately 200 nm into the specimen beyond
the coverglass interface. Olympus extends that leadership role by
offering four objectives for TIRFM including the world's highest N.A.
objective, the 100X N.A. 1.65 objective.
The incredibly thin optical section created be TIRFM allows an
extremely high signal to noise image to be collected. Popular
applications include vesicle tracking, cellular adhesions and single
molecule events.
• Olympus' original high N.A. objectives make it easy to produce an
evanescent wave field. So little light is leaked that a high-contrast
image can be obtained against a dark background.
◆
N.A. 1.65, 100X objective (APO100XOHR)
(Use special cover glass and immersion oil)
◆
N.A. 1.49, 60X objective (APON60XOTIRF)
(Use normal cover glass and immersion oil)
◆
N.A. 1.49, 100X objective (UAPON100XOTIRF)
(Use normal cover glass and immersion oil)
◆
N.A. 1.45 150X objective (UAPON150XOTIRF)
(Use normal cover glass and immersion oil)
• Once the initial alignment of the laser
optical path is set, it is just simple operation
switching between TIRF and widefield
illumination.
* TIRFM is a class 3B laser product.
Ultra-sensitive fluorescence microscopy.
Micrometer
TIRFM
Total Internal Reflection Fluorescence
observation with evanescent wave excitation
Widefield fluorescence observation with
mercury arc lamp excitation
Simultaneous laser light stimulation and imaging.
Laser Scanning Confocal
Application System
