background image

20

[ Frame plate adapter/
IX2-FP ]

This is used to fix the
microscope frame
to the anti-
vibration stand.

*Screws (available
separately) are required for
fixing.

[ Nosepiece stage/IX2-NPS]

This simple mechanical stage is designed for long time observations to minimize the
distance change between the specimen and the objective in other words ‘focus drift’. 
It works by minimizing the effect of temperature change and prevents blur during long
observations. Attach one objective in use.

[ Incubators ]

This box type incubator keeps the microscope temperature stable
with enclosing many components inside the box. Please see your
local supplier for more detail. 

[ Comparison of normal observation images ]

Change of focus when environmental temperature (25°C )
changes by ±5°C. 

*When the microscope is used without an incubator.

Accessories to improve stability in long-duration observations

[ CO

2

incubators/MIU-IBC-I, MIU-IBC-IF ]

Highly precise incubator control keeps the environment inside a
laboratory dish completely stable, at just below 37°C temperature,
90% moisture and 5% CO

2

concentration (when using a CO

2

5%

concentration bomb); in this way, live cell activity can be maintained
for about 2 days. A special designed structure is employed to
minimize the focus drift during temperature control. This is the ideal
solution for time-lapse experiments under both a confocal laser
scanning microscope and a wide field
observation. The opening hole located on
the top heater is available for the cell
injection.

* Built-in stage warming plate 
* Objective heater
* 5% CO2 supply tube with ø4 outer diameter, ø2 inner diameter
and 400 mm length.
* Not available in some areas

[ Thermoplate/MATS series ]

This thermoplate maintains the
temperature of the sample at 37°C.

* Tokai Hit Company products

MIU-IBC-IF

High grade configuration with a built-in
flowmeter for 5% CO

2

and 95% air. Use the 5%

CO

2

and 95% air bombs.

MIU-IBC-I

Basic configuration for control of heaters for
top, bath, stage and objective.

[ Comparison of fluorescence images ]

Time-lapse observation images. 

*When the microscope is used without an incubator.

With IX2-NPS

Normal observation images

With IX2-NPS

Normal observation images

20°C

25°C

30°C

0

30

60

120 min

Elapsed time

Environmental temperature

Live Cell Imaging System

19

Maintaining long-term stability for live cell observation.

Focus drift compensation function for 
time-lapse experiments.

Motorized inverted research microscope with 
focus drift compensation/IX81-ZDC

This landmark microscope model makes it easy to reproduce any
preset focus position. 785 nm weak laser light is introduced
through the additional optical path between the tube lens and an
objective to measure the distance between the objective and the
reflection plane, which is normally the boundary of the reflective
index difference such as the boundary between cover glass and
cell. Therefore this system never cause unnecessary
photobleaching of the specimen. 

Live cell imaging system

B.

Focusing

position

Reflection 
plane

Objective

Light
shielding
plate 

Split detector

Telan lens

Laser light
source 
(class 1)

A. 

Closer

than focus

position

C. 

Further

than focus

position

* Extended image

Содержание IX71

Страница 1: ...Laboratory for genomic Reprogramming Head of Laboratory Riken Kobe Institute Center for Developmental Biology CDB P13 Yuji Abe M D Ph D The 1st Department of Obstetrics Gynecology School of Medicine...

Страница 2: ...wavelength range and flat high transmittance this new system sets a new world standard of fluorescence performance efficiently detecting even faint fluorescence signals without damaging the cell optim...

Страница 3: ...lar transmission in the near infrared range is significantly enhanced Overall performance all across the wavelength range is ideally suited for today s most demanding research applications High N A ob...

Страница 4: ...put output of a parallel pencil of rays and the multiple port structure for gaining the primary image are designed internally in the form of tiers To maximize the possible wavelength width the optical...

Страница 5: ...filter wheels on the excitation and emission sides However this kind of mirror encounters the problem that each fluorescence image gets darker as the number of color dyes increase because the transmi...

Страница 6: ...g adapter U DULHA Allows simultaneous attachment of two light sources such as halogen and mercury Selection mirror is replaceable for custom applications Fluorescence illumination light source Reflect...

Страница 7: ...DICT U DICTS Thin specimen Big shearing value Thick specimen Small shearing value 10X 40X 100X U DICTHC for superior contrast with thin specimen observation U DICTHR for superior resolution with thic...

Страница 8: ...s DIC optical elements for IX2 MLWCD and applicable objectives 1 Objective with compensation for 1 mm plastic dish plus 0 5 mm thick thermoplate 2 Objective with compensation ring for 0 2 mm thick cov...

Страница 9: ...ffers different magnification without switching the objective lens 1 6X is standard IX71 81 and 2 0X is optional In addition to maximizing rigidity of IX2 microscope frame Olympus simplified or shorte...

Страница 10: ...pen close and camera vs visual observation Parfocal compensation function among objectives This function allows the focus point to be matched from low to high magnification objectives Refocusing each...

Страница 11: ...e cell injection Built in stage warming plate Objective heater 5 CO2 supply tube with 4 outer diameter 2 inner diameter and 400 mm length Not available in some areas Thermoplate MATS series This therm...

Страница 12: ...llumination with twin scanners TIRFM Total Internal Reflection Fluorescence Microscopy Since 1997 Olympus has been a market leader in objective based Total Internal Reflection microscope that allows a...

Страница 13: ...system from an arc lamp source ARC EVA SYSTEM DIAGRAM Main specifications Microscope Research inverted system microscope IX71 Fluorescence Arc illumination total internal reflection illuminator fluor...

Страница 14: ...order Easy system upgrade by attaching double lamphouse illuminator IX2 RFAW to IX2 series inverted microscope Photoactivation illuminator for inverted microscopy The novel Kaede gene is useful in bi...

Страница 15: ...urret IX2 RFAC 6 positions in a rotating turret built in shutter Light source 100 W Hg lamp housing and transformer or 75 W Xe lamp housing and transformer IX71 specifications Microscope body Revolvin...

Страница 16: ...tage IX SUSP Stage plate Incubator IX2 KSP Narrow plain stage CK2 SS Side stage U SIP Split primary image camera port Cube cassette for full image split images U IFFH Focus handle Interface U ZPCB2 Z...

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