NuAire LabGard NU-813-300, Руководство по эксплуатации и техническому обслуживанию

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OM0236 Page 21 of 52
February/2019
6.2 Operating Guidelines
6.2.1
The intent herein is to present general operating guidelines that will aid in the use of the CVE to control airborne
contaminants of low to moderate risk.
6.2.2
Procedure protocols defined in terms of the barrier of control concepts unique to the CVE must be developed in
order to obtain a maximum potential for safety and protection. The pre-planning necessary to develop these
protocols is based on several fundamental considerations, each of which will contribute to optimum benefits from
the equipment:
a. Minimize disruption of "air curtain"
b. Minimize room activity
c. Employ aseptic techniques
6.2.3 The minimum number of items necessary should be placed into the enclosure to prevent overloading, but the
work should also be planned to minimize the number of times an operator's hands and arms must enter and leave
the air curtain at the open face. The ideal situation is to have everything needed for the complete procedure place
in the enclosure’s workzone before starting, so that nothing need pass in or out through the air barrier at the face
until the procedure is completed. This is especially important in working with moderate risk agents.
Note: When working with agents of lower risk, it is not as important for all materials to be placed in the enclosure
workzone before starting, or for the procedure to be completely finished before materials are removed. Also, the
time period for a unit of work may be continued over a more extended period during which entries and
withdrawals from the enclosure may be made.
6.2.4 Minimize Room Activity
Activity in the room itself should be held to a minimum. Unnecessary activity may create disruptive air currents as
well as interfere with the work of the operator. A person walking past the front of CVE can cause draft velocities
up to 175 fpm (.88 m/s), which are sufficient to disrupt the air barrier provided by the work access opening.
6.2.5 Employ Methodical Technique
The operator must not assume an attitude of "let the CVE do it". Properly used, the CVE will do an excellent job of
containing viable or toxic agents. Normal laboratory contamination control procedures and basic methodical
techniques are necessary to obtain maximum benefit from the CVE. This precaution is merely an extension of
good laboratory technique as practiced on open bench tops. The good laboratory practices designed to minimize
creating and/or release of aerosols to the environment should not be discontinued.
Items of equipment in direct contact with powders or etiological agents must remain in the cabinet until enclosed
or until surface-decontaminated. Trays of discard work materials and other work aids must be covered or bagged
before removal from the enclosure workzone (aluminum foil may substitute for fabricated covers).
If an accident occurs which spills or splatters suspensions of chemicals or etiologic agents around the work area, all
surfaces and items in the enclosure workzone must be surface-decontaminated before being removed.
In brief, the consideration which should be made in order to obtain optimal personnel safety and product
protection may be reiterated:
a. Pre-plan the procedures carefully
b. Minimize disruption of the "air curtain"
c. Employ aseptic techniques
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