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OM0280 

Page 31 of 63 

Rev 3 July/2021 

7.1.1 

Manual Decon

 

1.  Disconnect power to the cabinet.  Remove screws at each upper side of the control center and allow the 

control center to rotate down, resting on the safety straps.  Disconnect electrical connectors on left side.  
Disconnect electrical from right side.  (Be sure to note the location of the supply and exhaust sensor wires 
before disconnecting them from the main board).  Remove control center by disconnecting safety straps 
lifting the control center up and away from cabinet.  Fold and pinch tubing to seal. 

2.  Remove the front decorative panel via top/front fasteners.   
3.  Remove window assembly (both frame and window, either being manual, sliding, or automatic) via the 

upper and lower fasteners (Refer to BCD-16376). At this point, the sliding window assembly can be 
removed. 

4.  Remove exhaust sensor shrouds.   
5.  Remove the diffuser screen and gasket around perimeter of workzone, if present. 
6.  Prepare decontamination equipment.  Reference decontamination procedure and use the following chart 

to calculate chemical requirements. 

 

 

Note:  The outlets in the work area are energized as long as the cabinet is plugged in and  

 

 

switched on the front panel. Unplug the cabinet before decontamination equipment is  

 

 

plugged into these outlets.  The control centers electrical connectors may be re-attached  

 

 

to utilize the cabinet's interior outlets and fan during the decontamination. 

7.  Set control center on its side resting on the left side of cabinet.  Then plug in the 16-pin connector cable 

from the cabinet to the control center.  Reconnect power to the cabinet.  The outlets and blower can then 
be activated.   

8.  Seal front and top openings using plastic and tape.   

 

 
 

 

 

 

 

BE SURE CABINET IS TOTALLY SEALED TO PREVENT ANY  

 

 

 

 

 

 

LABORATORY EXPOSURE TO DECONTAMINATION GAS. 

 
9.

 

 Perform decontamination procedure. 
 

 

Please consult with NuAire, Inc. about any unique contamination problems. 

 
 

Normally, no preventive maintenance is required on the interior of the cabinet (i.e., the area behind the 
access panel containing the HEPA filters and motor (blower assembly).  All required adjustments in order 
to maintain proper cabinet airflows are external to the cabinet interior.  The motor is lubricated for life 
and is thermally protected with automatic reset. 

 

7.2  Fluorescent/LED Lamp Replacement  

The two (T8) fluorescent bulbs or optional LED lamps are cool white and placed external to the cabinet to aid maintenance 
and minimize heat build-up within the cabinet.  The life rating of the bulb is 9,000 for fluorescent and 50,000 hours for LED 
based on three-hour burning cycles. 
 
To replace a lamp, it is necessary to remove the lamp assembly. 

1.  Switch cabinet light switch off. 
2.  Remove the screws at each upper side of the control center and allow the control center to rotate down, resting 

on the safety straps. 

3.  The lamp is now directly exposed for replacement. 
4.  The lamp is removed by displacing the bulb to one side against the compressible bulb holder and lifting out the 

lamp. 

5.  Reverse the procedure to reinstall the lamp assembly being careful not to pinch the safety straps, cable or tubing 

during closure of the control center. 

 

¹ Available from Lab Safety Supply, Janesville, WI 53547-1368, or other laboratory, industrial, or hospital supply distributor 

Cabinet Size 

300E 

400E 

500E 

600E 

Cabinet 

Dimensions 

58-7/8 x 28 x 34-3/8 in. 

(1.50 x .711 x .873 m) 

58-7/8 x 28 x 46-3/8 

(1.50 x .711 x 1.18 m) 

58-7/8 x 28 x 58-3/8 

(1.50 x .711 x 1.48m) 

58-7/8 x 28 x 70-3/8 

(1.50 x .739 x 1.88 m) 

Cabinet 
Volume 

32.79 cu. ft. 
(.929 cu. m) 

44.24 cu. ft. 
(1.25 cu. m) 

55.69 cu. ft. 
(1.58 cu. m) 

67.14 cu. ft. 
(1.90 cu. m) 

 

CAUTION 

Содержание LabGard NU-540-300E

Страница 1: ...abinet Models NU 540 300E 400E 500E 600E Bench Console Operation and Maintenance Manual July 2021 Revision 3 NU 540 300E 400E 500E 600E Series F Manufactured By NuAire Inc 2100 Fernbrook Lane Plymouth...

Страница 2: ...m particulate contaminants as prescribed in EN 12469 and NSF ANSI 49 Please read this manual carefully to familiarize yourself with proper installation maintenance and operation of the cabinet Other r...

Страница 3: ...ings are appended in the back flap of this manual along with any modifications and or additions to procedures as outlined in this manual A copy of the original factory test report is also appended to...

Страница 4: ...eak test 7 7 Airflow Smoke Pattern Test 7 8 Site Installation Assessment Test 7 9 Cleanliness Classification Test for Pharmacy Application 7 10 Main Control Board Description and Replacement Section N...

Страница 5: ...Airflow Schematic and the application of environmental controls as required in the field of biological research or chemical containment The LFBSC when used with proper technique is an effective primar...

Страница 6: ...while specific agents are used o which measures are to be taken in the case of an accident Repairs to the device must be carried out only by trained and authorized expert personnel Keep these operatin...

Страница 7: ...OM0280 Page 7 of 63 Rev 3 July 2021...

Страница 8: ...OM0280 Page 8 of 63 Rev 3 July 2021 2 0 Models and Features The model NU 540E LabGard ES Class II Laminar Flow Biosafety Cabinet is manufactured in four sizes 3 ft 9m 4 ft 1 2m 5 ft 1 5m and 6 ft 1 8m...

Страница 9: ...OM0280 Page 9 of 63 Rev 3 July 2021...

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Страница 13: ...Shipments 4 1 1 Terms are factory unless stated otherwise Therefore it is important to check each shipment before acceptance 4 1 2 If there is visible damage the material can be accepted after the dri...

Страница 14: ...aintenance purposes Do not position the cabinet to prevent access to the power cord The power cord plug serves as the disconnect and should remain readily accessible If the outlet is inaccessible such...

Страница 15: ...ENTS i e locking type OF DRAIN VALVE INSTALLATION remove the handle from the valve stem to gain clearance for valve body rotation Add Loctite 242 furnished to the threads and rotate valve body until s...

Страница 16: ...OM0280 Page 16 of 63 Rev 3 July 2021...

Страница 17: ...uld not be used Do not attempt to clean the HEPA filter media Cabinet interior walls or work surface are easily cleaned with any mild household detergent cleaner using a soft cloth Turn the cabinet on...

Страница 18: ...Sidewall One Backwall Left or Right Work Surface Cabinet Size Inches mm Height Fully Assembled Height Minimum for Transport Width Depth with Armrest Removed 60 7 8 1546 59 1499 41 5 8 1057 31 7 16 79...

Страница 19: ...compensation constant volume control for both filter loading and line voltage variances Monitor and display airflow system performance via PresurFlow monitor The LabGard ES NU 540E offers the latest d...

Страница 20: ...OM0280 Page 20 of 63 Rev 3 July 2021...

Страница 21: ...bove the key indicates full blue for fluorescent light on 6 1 2 4 Ultraviolet UV Light Key The UV light key controls the on off power to the UV light if optionally installed LED above indicates full y...

Страница 22: ...e cabinet airflow status During the warm up period the aseptic cleaning process may begin If the sliding window is raised an audible and visual alarm will occur but may be silenced by pressing the ala...

Страница 23: ...r expires the function will turn off Select auto timer duration function o Outlets Press and hold key then press hidden outlet outlet keys sequentially LED indicator above outlet will blink fast Adjus...

Страница 24: ...blower hidden keys sequentially Red LED indicator above blower will blink fast Select password Press or key to scroll through the code choices below No Password B B H password B H B password default B...

Страница 25: ...should be evaluated by appropriate safety personnel 6 2 2 Minimize Penetration of Air Curtain The minimum number of items necessary should be placed into the cabinet to prevent overloading but the wor...

Страница 26: ...precaution is merely an extension of good aseptic technique as practiced on open bench tops The standard laboratory practices designed to minimize creation and or release of aerosols to the environmen...

Страница 27: ...lls Materials should be arranged so that clean dirty used and virus materials are well separated Passage of contaminated materials over uninoculated cultures or clean glassware should be avoided and t...

Страница 28: ...re feet are resting on the floor chair foot support or foot rest Also be sure back is fully supported with proper chair adjustments Forearm elbow support The cabinet is provided with a non metallic fo...

Страница 29: ...I LFBSC can be found in the 2006 ASHP Technical Assistance Bulletin ASHP Guidelines on Handling Hazardous Drugs2 6 6 2 Procedure a Make sure that the cabinet remains in operational mode with internal...

Страница 30: ...ly decontaminated is known to be biologically clean or known to be chemically inert Surface disinfection is performed as specified in the Cleaning Procedures section Hazardous Gases Personal Protectio...

Страница 31: ...E SURE CABINET IS TOTALLY SEALED TO PREVENT ANY LABORATORY EXPOSURE TO DECONTAMINATION GAS 9 Perform decontamination procedure Please consult with NuAire Inc about any unique contamination problems No...

Страница 32: ...lock washers The screws have O rings and should be replaced if damaged or badly deformed The interior of the cabinet is now fully exposed for replacement of the filters and motor blower The lowest wi...

Страница 33: ...x 32 813mm x 3 76mm 20 508mm x 14 366mm x 6 152mm Filter Manufacturer Camfil Farr Camfil Farr NU 540 400E NuAire Part Number A 980979 01 A 980957 18 Filter Size 21 533mm x 44 1118mm x 3 76mm 20 508mm...

Страница 34: ...top of the hood Tighten the spring loaded bolts 4 places depressing the gasket material by 1 8 inch 3mm Step 5 Motor Blower Assembly Removal a It is recommended that the motor blower to be removed as...

Страница 35: ...perly activate the micro switches thus causing potential operational malfunctions to occur If the window counter balance experiences a fault window function must be verified the sliding window retenti...

Страница 36: ...model size Class II 3 foot Class II 4 foot Class II 5 foot Class II 6 foot Laminar 3 4 foot Laminar 5 6 foot o Press hidden key to accept model size and exit o Press key at any time to abort and exit...

Страница 37: ...adjusts or balances the exhaust airflow as well as makes up for filter resistance tolerances Since it has been NuAire s experience that the filters may not load evenly choke adjustments may be necess...

Страница 38: ...justment 7 5 3 2 Inflow Calibration Step 1 Measure the inflow velocity using the recommended procedure found in Table 7 0 If necessary adjust the exhaust filter choke located under the front decorativ...

Страница 39: ...must be non blinking to save or exit Note At any time during the process Press hidden key to accept low alarm setpoint value Press and hold outlet key for three seconds to remove any previous offsets...

Страница 40: ...not go through normal start up procedure 7 5 3 5 Nite Care Calibration See Section 8 2 2 to Activate the Nite Care Function The Nite Care mode is defaulted to operate the blower at approximately 600 r...

Страница 41: ...18 750 476 18 0 457 17 750 451 17 5 445 Number of Readings Average Velocity ft min m s d Acceptance Criteria 1 Average downflow velocity 55 to 65 fpm 28 to 33 m s 2 Individual readings must be within...

Страница 42: ...3 386 37 584 41 782 45 980 50 178 54 375 102 208 315 421 528 635 741 848 955 1061 1168 1274 1381 600E 4 8 158 12 316 16 474 20 632 24 790 28 948 33 106 37 264 41 422 45 580 49 738 53 896 58 054 62 212...

Страница 43: ...fficult to check since protective grills charcoal filters or exhaust transitions could cover the filter Access panels are usually provided and should be removed If an air gap exhaust transition is pro...

Страница 44: ...shall show smooth downward flow with no dead spots or reflux 2 No smoke shall escape from inside the cabinet 3 No smoke refluxes out of the cabinet once drawn in nor does smoke billow over the worksur...

Страница 45: ...ts certified operating voltage as measured by the voltmeter 7 8 3 Exhaust System Performance Canopy Exhaust Transitions Step 1 Introduce a visible medium source into the canopy air intake s while slow...

Страница 46: ...test using non hazardous materials A minimum of three 3 1 minute particle counts shall be sampled and recorded while the user simulates aseptic compounding manipulations At Rest Particle Count Test Ta...

Страница 47: ...MPS 2 AMPS 1 AMP 2 NU 540 400E 6 25 AMPS 3 AMPS 2 AMPS 1 AMP 2 NU 540 500E 8 AMPS 3 AMPS 2 AMPS 1 AMP 2 NU 540 600E 8 AMPS 3 AMPS 2 AMPS 1 AMP 2 7 10 3 Main Control Board Replacement Note All setup an...

Страница 48: ...he initial power up the RED alarm LED will blink to indicate power up status 5 Press key to clear the power on reset message 6 Enter into service parameters and input configuration for calibration and...

Страница 49: ...utlet fuse located on main control board Check voltage coming out of main control board Cabinet ultraviolet light won t turn on Yellow LED above UV light key indicates the UV lamp should be on Check s...

Страница 50: ...running for the accessary outlet to turn on Sync Outlet Power with Active Blower Normally the outlet power is turned on via the outlet key If the outlet power sync is active the outlet power will turn...

Страница 51: ...running Upon closure the blower will continue to run at a calibrated lower speed level to keep the workzone interior sterile The PresurFlow will indicate s blinking green LEDs along with green LED ab...

Страница 52: ...re activated whenever the blower key is pressed and the blower key LED indicator is on or blinking Contact ratings are 250 VAC maximum at 2 Amps 9 2 Alarm Relay The alarm relay contacts are normally o...

Страница 53: ...June 1976 Since ultraviolet rays will not penetrate ordinary glass it is recommended that the sliding window be closed while the ultraviolet light is on within the cabinet or that personnel leave the...

Страница 54: ...form the following Press and hold key the press UV light UV light UV light key sequentially and the reset will occur removing the above alarm condition It is also possible to approximate the UV light...

Страница 55: ...ep hermolyticus alpha type 5 500 Staphylococcus aureus 6 600 Staphylococcus albus 5 720 Micrococcus sphaeroides 15 400 Sarcina lutea 26 400 Pseudomonas fluorescens 7 040 Escherichia coli 7 040 Proteus...

Страница 56: ...lic mains such as hospital and research laboratories and most industrial laboratories the expected transient overvoltage is 2500 V for a 230 V supply and 1500 V for a 120 V supply 11 5 Pollution Degre...

Страница 57: ...t Grill Stainless Steel Worksurface Stainless Steel Window Faring Stainless Steel Window Glides HDPE Window Safety Glass Window Frame Stainless Steel Front Service Panel Painted Steel Front Decorative...

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