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OM0280 

Page 15 of 63 

Rev 3 July/2021 

 

5.2  Set-Up Instructions 

Remove outer shipping protection (carton or crating).  The cabinet is fastened to the base skid and it is usually the best 
procedure to leave the skid in place until the cabinet is located in its approximate position to facilitate ease in handling.  It 
can then be removed from the skid by removing the banding, bolts and screws holding the cabinet to the skid.  It may be 
necessary to remove the control center in order to gain passage through a doorway.  It can easily be removed by following 
the instructions on drawing BCD-16301.   
 
 

 

 

 

 

It is recommended that no less than two people are present using a 

 

 

 

 

 

lifting system for placement of the cabinet onto the base stand.   

 

 

 

 

 

It is not recommended to manually lift the cabinet onto the base stand. 

 

5.2.1 

Base Stand Assembly 

The base stand is typically shipped knocked down in a separate carton and is assembled per drawing BCD-16385 
Once assembled; place the stand in approximate position (ready for cabinet placement onto the base stand). Now 
lift the cabinet on top of the base stand  and bolt the base stand to the cabinet using two 3/8" - 16 x 3/4" bolts and 
washers provided for the front base stand tabs and two 1/4" acorn nuts for the rear weld studs.  Place the cabinet 
in the desired location. 
 

5.2.2 

Leveling 

Using a level placed on the work tray, first adjust the leg levelers end to end and then front to back.  The NSF 
approved leg levelers provide a 

 3/4" (20mm) adjustment. 

 

5.2.3 

Bench Installation

 (BCD-16882) 

Place the cabinet on the bench with approximately a 2" (51mm) overhang clearance for installation of the drain 
valve.  Place the cabinet in desired location and using RTV caulk, seal all around the base of the cabinet and the 
bench.  This provides a tight seal to prevent bench spills from migrating under the cabinet. 
 
If the drain valve is desired, (NOTE, CHECK WITH YOUR SAFETY PERSONNEL FOR REGULATORY REQUIREMENTS (i.e. 
locking type) OF DRAIN VALVE INSTALLATION) remove the handle from the valve stem to gain clearance for valve 
body rotation.  Add Loctite 242 (furnished) to the threads and rotate valve body until secure, with the valve stem 
(for handle) on the left side.  Re-install handle to valve stem.  Adjust the cabinet on bench to provide a 2" (51mm) 
overhang and seal the interface of the bench and cabinet, using RTV caulk as above. 

 

5.2.4 

Gas Service 

NuAire doesn't recommend the use of natural gas within the LFBSC, but if gas service is determined to be 
necessary for the application, appropriate safety measures must take place.  All NuAire LFBSC's have precautionary 
warning labels that say the following: 

                                            

 

Use of explosive or flammable substances in this cabinet  

 

                                should be evaluated by your appropriate safety personnel. 

 

Once the determination has been made by the appropriate safety personnel, the application of natural gas must 
be performed in accordance to national, state and local codes.  IT IS ALSO STRONGLY RECOMMENDED THAT AN 
EMERGENCY GAS SHUTOFF VALVE BE PLACED JUST OUTSIDE THE LFBSC ON THE GAS SUPPLY LINE. 
 
The gas valve, when this option is installed, will only operate or flow gas when the cabinet blower is on and no 
alarm is present.  A solenoid valve is installed on the gas supply line for this purpose. 

 

Note: Some countries (i.e. Germany) only allow certain types of certified valves to be used for natural gas  
           (i.e. Germany DVGW Certified).  Verify requirements per Local, State and Federal codes/laws. 

 
As previously stated NuAire doesn't recommend the use of natural gas within the LFBSC and ASSUMES NO 
RESPONSIBILITY FOR ITS USE.  USE AT YOUR OWN RISK.  The Bunsen burner flame within the LFBSC not only 
contributes to heat build-up; it also disrupts the laminar air stream, which must be maintained for maximum 
efficiency.  IF THE PROCEDURE DEMANDS USE OF A FLAME, A BUNSEN BURNER WITH ON DEMAND IGNITION IS 
STRONGLY RECOMMENDED.  DO NOT USE CONSTANT FLAME GAS BURNERS.  During use, the Bunsen burner 
should be placed to the rear of the workspace where resulting air turbulence will have a minimal effect. 

 

 

CAUTION 

CAUTION 

 

Содержание LabGard NU-540-300E

Страница 1: ...abinet Models NU 540 300E 400E 500E 600E Bench Console Operation and Maintenance Manual July 2021 Revision 3 NU 540 300E 400E 500E 600E Series F Manufactured By NuAire Inc 2100 Fernbrook Lane Plymouth...

Страница 2: ...m particulate contaminants as prescribed in EN 12469 and NSF ANSI 49 Please read this manual carefully to familiarize yourself with proper installation maintenance and operation of the cabinet Other r...

Страница 3: ...ings are appended in the back flap of this manual along with any modifications and or additions to procedures as outlined in this manual A copy of the original factory test report is also appended to...

Страница 4: ...eak test 7 7 Airflow Smoke Pattern Test 7 8 Site Installation Assessment Test 7 9 Cleanliness Classification Test for Pharmacy Application 7 10 Main Control Board Description and Replacement Section N...

Страница 5: ...Airflow Schematic and the application of environmental controls as required in the field of biological research or chemical containment The LFBSC when used with proper technique is an effective primar...

Страница 6: ...while specific agents are used o which measures are to be taken in the case of an accident Repairs to the device must be carried out only by trained and authorized expert personnel Keep these operatin...

Страница 7: ...OM0280 Page 7 of 63 Rev 3 July 2021...

Страница 8: ...OM0280 Page 8 of 63 Rev 3 July 2021 2 0 Models and Features The model NU 540E LabGard ES Class II Laminar Flow Biosafety Cabinet is manufactured in four sizes 3 ft 9m 4 ft 1 2m 5 ft 1 5m and 6 ft 1 8m...

Страница 9: ...OM0280 Page 9 of 63 Rev 3 July 2021...

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Страница 13: ...Shipments 4 1 1 Terms are factory unless stated otherwise Therefore it is important to check each shipment before acceptance 4 1 2 If there is visible damage the material can be accepted after the dri...

Страница 14: ...aintenance purposes Do not position the cabinet to prevent access to the power cord The power cord plug serves as the disconnect and should remain readily accessible If the outlet is inaccessible such...

Страница 15: ...ENTS i e locking type OF DRAIN VALVE INSTALLATION remove the handle from the valve stem to gain clearance for valve body rotation Add Loctite 242 furnished to the threads and rotate valve body until s...

Страница 16: ...OM0280 Page 16 of 63 Rev 3 July 2021...

Страница 17: ...uld not be used Do not attempt to clean the HEPA filter media Cabinet interior walls or work surface are easily cleaned with any mild household detergent cleaner using a soft cloth Turn the cabinet on...

Страница 18: ...Sidewall One Backwall Left or Right Work Surface Cabinet Size Inches mm Height Fully Assembled Height Minimum for Transport Width Depth with Armrest Removed 60 7 8 1546 59 1499 41 5 8 1057 31 7 16 79...

Страница 19: ...compensation constant volume control for both filter loading and line voltage variances Monitor and display airflow system performance via PresurFlow monitor The LabGard ES NU 540E offers the latest d...

Страница 20: ...OM0280 Page 20 of 63 Rev 3 July 2021...

Страница 21: ...bove the key indicates full blue for fluorescent light on 6 1 2 4 Ultraviolet UV Light Key The UV light key controls the on off power to the UV light if optionally installed LED above indicates full y...

Страница 22: ...e cabinet airflow status During the warm up period the aseptic cleaning process may begin If the sliding window is raised an audible and visual alarm will occur but may be silenced by pressing the ala...

Страница 23: ...r expires the function will turn off Select auto timer duration function o Outlets Press and hold key then press hidden outlet outlet keys sequentially LED indicator above outlet will blink fast Adjus...

Страница 24: ...blower hidden keys sequentially Red LED indicator above blower will blink fast Select password Press or key to scroll through the code choices below No Password B B H password B H B password default B...

Страница 25: ...should be evaluated by appropriate safety personnel 6 2 2 Minimize Penetration of Air Curtain The minimum number of items necessary should be placed into the cabinet to prevent overloading but the wor...

Страница 26: ...precaution is merely an extension of good aseptic technique as practiced on open bench tops The standard laboratory practices designed to minimize creation and or release of aerosols to the environmen...

Страница 27: ...lls Materials should be arranged so that clean dirty used and virus materials are well separated Passage of contaminated materials over uninoculated cultures or clean glassware should be avoided and t...

Страница 28: ...re feet are resting on the floor chair foot support or foot rest Also be sure back is fully supported with proper chair adjustments Forearm elbow support The cabinet is provided with a non metallic fo...

Страница 29: ...I LFBSC can be found in the 2006 ASHP Technical Assistance Bulletin ASHP Guidelines on Handling Hazardous Drugs2 6 6 2 Procedure a Make sure that the cabinet remains in operational mode with internal...

Страница 30: ...ly decontaminated is known to be biologically clean or known to be chemically inert Surface disinfection is performed as specified in the Cleaning Procedures section Hazardous Gases Personal Protectio...

Страница 31: ...E SURE CABINET IS TOTALLY SEALED TO PREVENT ANY LABORATORY EXPOSURE TO DECONTAMINATION GAS 9 Perform decontamination procedure Please consult with NuAire Inc about any unique contamination problems No...

Страница 32: ...lock washers The screws have O rings and should be replaced if damaged or badly deformed The interior of the cabinet is now fully exposed for replacement of the filters and motor blower The lowest wi...

Страница 33: ...x 32 813mm x 3 76mm 20 508mm x 14 366mm x 6 152mm Filter Manufacturer Camfil Farr Camfil Farr NU 540 400E NuAire Part Number A 980979 01 A 980957 18 Filter Size 21 533mm x 44 1118mm x 3 76mm 20 508mm...

Страница 34: ...top of the hood Tighten the spring loaded bolts 4 places depressing the gasket material by 1 8 inch 3mm Step 5 Motor Blower Assembly Removal a It is recommended that the motor blower to be removed as...

Страница 35: ...perly activate the micro switches thus causing potential operational malfunctions to occur If the window counter balance experiences a fault window function must be verified the sliding window retenti...

Страница 36: ...model size Class II 3 foot Class II 4 foot Class II 5 foot Class II 6 foot Laminar 3 4 foot Laminar 5 6 foot o Press hidden key to accept model size and exit o Press key at any time to abort and exit...

Страница 37: ...adjusts or balances the exhaust airflow as well as makes up for filter resistance tolerances Since it has been NuAire s experience that the filters may not load evenly choke adjustments may be necess...

Страница 38: ...justment 7 5 3 2 Inflow Calibration Step 1 Measure the inflow velocity using the recommended procedure found in Table 7 0 If necessary adjust the exhaust filter choke located under the front decorativ...

Страница 39: ...must be non blinking to save or exit Note At any time during the process Press hidden key to accept low alarm setpoint value Press and hold outlet key for three seconds to remove any previous offsets...

Страница 40: ...not go through normal start up procedure 7 5 3 5 Nite Care Calibration See Section 8 2 2 to Activate the Nite Care Function The Nite Care mode is defaulted to operate the blower at approximately 600 r...

Страница 41: ...18 750 476 18 0 457 17 750 451 17 5 445 Number of Readings Average Velocity ft min m s d Acceptance Criteria 1 Average downflow velocity 55 to 65 fpm 28 to 33 m s 2 Individual readings must be within...

Страница 42: ...3 386 37 584 41 782 45 980 50 178 54 375 102 208 315 421 528 635 741 848 955 1061 1168 1274 1381 600E 4 8 158 12 316 16 474 20 632 24 790 28 948 33 106 37 264 41 422 45 580 49 738 53 896 58 054 62 212...

Страница 43: ...fficult to check since protective grills charcoal filters or exhaust transitions could cover the filter Access panels are usually provided and should be removed If an air gap exhaust transition is pro...

Страница 44: ...shall show smooth downward flow with no dead spots or reflux 2 No smoke shall escape from inside the cabinet 3 No smoke refluxes out of the cabinet once drawn in nor does smoke billow over the worksur...

Страница 45: ...ts certified operating voltage as measured by the voltmeter 7 8 3 Exhaust System Performance Canopy Exhaust Transitions Step 1 Introduce a visible medium source into the canopy air intake s while slow...

Страница 46: ...test using non hazardous materials A minimum of three 3 1 minute particle counts shall be sampled and recorded while the user simulates aseptic compounding manipulations At Rest Particle Count Test Ta...

Страница 47: ...MPS 2 AMPS 1 AMP 2 NU 540 400E 6 25 AMPS 3 AMPS 2 AMPS 1 AMP 2 NU 540 500E 8 AMPS 3 AMPS 2 AMPS 1 AMP 2 NU 540 600E 8 AMPS 3 AMPS 2 AMPS 1 AMP 2 7 10 3 Main Control Board Replacement Note All setup an...

Страница 48: ...he initial power up the RED alarm LED will blink to indicate power up status 5 Press key to clear the power on reset message 6 Enter into service parameters and input configuration for calibration and...

Страница 49: ...utlet fuse located on main control board Check voltage coming out of main control board Cabinet ultraviolet light won t turn on Yellow LED above UV light key indicates the UV lamp should be on Check s...

Страница 50: ...running for the accessary outlet to turn on Sync Outlet Power with Active Blower Normally the outlet power is turned on via the outlet key If the outlet power sync is active the outlet power will turn...

Страница 51: ...running Upon closure the blower will continue to run at a calibrated lower speed level to keep the workzone interior sterile The PresurFlow will indicate s blinking green LEDs along with green LED ab...

Страница 52: ...re activated whenever the blower key is pressed and the blower key LED indicator is on or blinking Contact ratings are 250 VAC maximum at 2 Amps 9 2 Alarm Relay The alarm relay contacts are normally o...

Страница 53: ...June 1976 Since ultraviolet rays will not penetrate ordinary glass it is recommended that the sliding window be closed while the ultraviolet light is on within the cabinet or that personnel leave the...

Страница 54: ...form the following Press and hold key the press UV light UV light UV light key sequentially and the reset will occur removing the above alarm condition It is also possible to approximate the UV light...

Страница 55: ...ep hermolyticus alpha type 5 500 Staphylococcus aureus 6 600 Staphylococcus albus 5 720 Micrococcus sphaeroides 15 400 Sarcina lutea 26 400 Pseudomonas fluorescens 7 040 Escherichia coli 7 040 Proteus...

Страница 56: ...lic mains such as hospital and research laboratories and most industrial laboratories the expected transient overvoltage is 2500 V for a 230 V supply and 1500 V for a 120 V supply 11 5 Pollution Degre...

Страница 57: ...t Grill Stainless Steel Worksurface Stainless Steel Window Faring Stainless Steel Window Glides HDPE Window Safety Glass Window Frame Stainless Steel Front Service Panel Painted Steel Front Decorative...

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