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OM0301

August 20

Page 95 of 105

Energies Required to Destroy Some Microorganisms By Ultraviolet Radiations(e)

Mold Spores

Microwatt

seconds

per cm/2

Protozoa

Microwatt

seconds

per cm/2

Penicillum roqueforti

26,400 Paramecium

200,000(a)

Penicillium expansum

22,000

Penicillium digitiatum

88,000

Nematode Eggs

40,000(b)

Aspergillus glaucus

88,000

Aspergillus flavus

99,000

Algae

22,000(c)

Aspergillus niger

330,000

Rhizopus nigricans

220,000

Virus

Mucor racemosus A

35,200 Baceriophage (E. Coli)

6,600

Mucor racemosus B

35,200 Tobacco Masaic

440,000

Oospora lactis

11,000 Influenze

3,400(d)

Yeasts

Saccharomyces

13,200

ellipsoideus

17,600

Saccharomyces cerevisiae

13,200

Brewers' yeast

6,600

Baker's yeast

8,800

Common yeast cake

13,200

Bacteria

Streptococcus lactis

8,800

Strep. hermolyticus (alpha type)

5,500

Staphylococcus aureus

6,600

Staphylococcus albus

5,720

Micrococcus sphaeroides

15,400

Sarcina lutea

26,400

Pseudomonas fluorescens

7,040

Escherichia coli

7,040

Proteus vulgaris

7,480

Serratia marcescens

6,160

Bacillus subtilis

11,000

Bacillus subtilis spores

22,000

Spirillum rubrum

6,160

References:

(a) Luckiesh, Matthew (1946) Application of Germicidal, Ethyemal and Infrared Energy, D. Van Nostrand o., New York, New York, pp 253

(b) Hollaender (1942) Aerobiology, A.A.A.S. (for 90% inactivation), pp 162

(d) Hollaender, A., Oliphant, J.W. (1944) The inactivation effect of monochromatic ultraviolet. Radiation on Influenza Virus (for 90% inactivation)

Jour. of Bact. 48, pp. 447-454

(e) This table, "Energies Required to Destroy Some Microorganisms by Ultraviolet Radiations" comes from Westinghouse brochure entitled - "Westinghouse

Sterilamp Germicidal Ultraviolet Tubes"

Содержание LabGard ES NU-565-400

Страница 1: ...Flow Biosafety Fume Hood Cabinet Models NU 565 400 600 Bench Console Operation Maintenance Manual Series C Revision 1 August 2020 Manufactured By Nuaire Inc 2100 Fernbrook Lane Plymouth MN 55447 Toll...

Страница 2: ...m particulate contaminants as prescribed in NSF ANSI 49 Please read this manual carefully to familiarize you with proper installation maintenance and operation of the cabinet Other reference and guide...

Страница 3: ...p drawings are appended in the back flap of this manual along with any modifications and or additions to procedures as outlined in this manual A copy of the original factory test report is also append...

Страница 4: ...EPA Filter Leak Test 7 7 Airflow Smoke Pattern Test 7 8 Site Installation Assessment Tests 7 9 Cleanliness Classification Test for Pharmacy Application 7 10 Main Control Board Description Replacement...

Страница 5: ...d with proper technique is an effective laboratory aid in obtaining the optimum control over product quality while reducing the potential for exposure of both product and personnel to airborne biologi...

Страница 6: ...apply while specific agents are used o Which measures are to be taken in the case of an accident Repairs to the device must be carried out only by trained and authorized expert personnel Keep these op...

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Страница 8: ...OM0301 August 20 Page 8 of 105 2 0 Models Features The model NU 565 LABGARD ES Class II Type B2 Laminar Flow Biosafety Fume Hood Cabinet is manufactured in two sizes 4 ft 1 2m and 6 ft 1 8m...

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Страница 12: ...ipments 4 1 1 Terms are factory unless stated otherwise Therefore it is important to check each shipment before acceptance 4 1 2 If there is visible damage the material can be accepted after the drive...

Страница 13: ...accessible If the outlet is inaccessible such as a conduit hardwired connection then an appropriate warning label should be applied near the cabinet s on off switch to indicate the circuit breaker on...

Страница 14: ...ecure See BCD 16300 5 2 2 Leveling Using a level placed on the work tray adjust the leg levelers first end to end then front to back The NSF approved leg levelers provide a 3 4 20mm adjustment 5 2 3 B...

Страница 15: ...emote controlled needle valve plumbing fixtures Nuaire provides for rear bottom or top connections of plumbing services to plant utilities Connection from the needle valve assembly to the welded exit...

Страница 16: ...e A minimum of 20 gauge for round duct is required to prevent duct collapse due to high static pressure conditions square duct will require heavier gauge material 8 All ductwork should be maintained a...

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Страница 19: ...ume and corresponding inflow velocity is displayed on the front panel Preset lower and upper alarm limits are factory set but can be field verified at any time To ensure that adequate exhaust is avail...

Страница 20: ...tch operation utilizing independent exhaust volume measurement instrument DIM Per NSF ANSI 49 a 20 loss of exhaust volume must produce an airflow alarm within 15 seconds The testing methods and equipm...

Страница 21: ...bled Incl Exh Filter Fastener Height Minimum for Transport Width Depth with Armrest removed 62 1575 62 1575 53 5 8 1362 32 3 8 823 62 1575 62 1575 77 5 8 1972 32 3 8 823 Work Access Opening Inches mm...

Страница 22: ...ital dual thermistor airflow sensor in the downflow stream to monitor and control airflow to setpoints The control system automatically compensates for filter loading voltage variances and other envir...

Страница 23: ...be accessed for additional user menus 6 1 3 Run Mode Anytime the blower icon is selected the Run Mode screen will appear The Run Mode screen will display set points and the countdown of a 2 minute war...

Страница 24: ...w the icons is the message area that will indicate alarms errors or other notable conditions Since the message area is limited to one line of text only the highest priority message will be indicated H...

Страница 25: ...sed will provide a list of menu items for various TouchLink functions 6 1 5 1 Setpoint Limits This menu screen will indicate the Biosafety Fume Hood Cabinet airflow control setpoints Access is restric...

Страница 26: ...setting text will provide a list of time functions available for use below is a description of each timer function Timer Functions Purge Timer This timer controls how long the blower will run to purge...

Страница 27: ...UV light If timer is zero UV light will stay on until manually turned OFF or window is opened breaking the safety interlock Auto Run Timers This timer provides the ability to program on a daily basis...

Страница 28: ...age 28 of 105 Once into the selected day press on window to enter the on off times Use the keypad to enter hours or minutes Press menu icon SAVE after each time entry Repeat auto timer function for ea...

Страница 29: ...5 3 Historical Performance When press a historical performance text the system displays downflow and inflow history Downflow History press downflow history the system displays the week log data in gra...

Страница 30: ...Inflow History press downflow history the system displays the week log data in graphical form of Inflow versus days Exhaust History press Exhaust history the system displays the week log data in graph...

Страница 31: ...r service personnel to set up and calibrate the cabinet for certification or commissioning 6 1 5 5 Display Setting This menu item provides the ability to alter LCD screen display background contract a...

Страница 32: ...d Gray can be selected Touch display tone audible feedback can turn on off Display brightness can be adjusted Languages may be selected of English Spanish French and German Time Date This menu item pr...

Страница 33: ...0301 August 20 Page 33 of 105 6 1 5 6 Change User Password This menu item provides the ability to change the user password from the default value of 1234 Set Password Enter Old Password Set New Passwo...

Страница 34: ...OM0301 August 20 Page 34 of 105 Retype New Password 6 1 5 7 System Information This menu item provides the cabinet model number serial number and software revision...

Страница 35: ...ding window can be closed and the UV light turned on if installed NOTE If night setback exhaust airflow is reduced by the Building Automation System BAS and not by the Nuaire Model NU 951 012 valve it...

Страница 36: ...by appropriate safety personnel 6 2 2 Minimize Penetration of Air Curtain The minimum number of items necessary should be placed into the cabinet to prevent overloading but the work should also be pl...

Страница 37: ...s precaution is merely an extension of good aseptic technique as practiced on open bench tops The good laboratory practices designed to minimize creation and or release of aerosols to the environment...

Страница 38: ...minated materials over uninoculated cultures or clean glassware should be avoided and transfer of viable materials should be performed as deeply into the cabinet away from open face as possible 6 3 4...

Страница 39: ...ing seat and back control for personalized adjustment to assure proper user posture Be sure feet are resting on the floor chair foot support or foot rest Also be sure back is fully supported with prop...

Страница 40: ...position With the view screen in the full open position personnel protection is compromised and a full faced HEPA filtered respirator must be worn c Clean all readily accessible surfaces of the cabin...

Страница 41: ...f the sample chamber in the safety cabinet that is to be disinfected The formaldehyde evaporates immediately after reaching its boiling point the minimum reaction time is 6 hours Therefore the formald...

Страница 42: ...s Normally no preventive maintenance is required on the interior of the cabinet i e the area behind the access panel containing the HEPA filters and motor blower assembly All required adjustments in o...

Страница 43: ...e exhaust system 7 3 1 Supply Filter Replacement see Drawing BCD 19032 Disconnect electrical power from the cabinet before attempting any maintenance action Step 1 Remove screws at each upper side of...

Страница 44: ...PA seal frame clockwise until the gasket is visually depressed by 1 8 inch 3mm The procedure for replacing the exhaust filter with the Bag in Bag out option is shown on Drawing BCD 05660 7 3 3 Motor B...

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Страница 46: ...at the airflow monitor sensors are calibrated to read the correct values Press MENU to access Calibration Service parameter Entry into the Calibration Service functions requires a service password for...

Страница 47: ...n the control system and is factory set and shouldn t require alteration The cabinet type default information controls unit of measure setpoints and limits based on the type and size of cabinet The OE...

Страница 48: ...flow area workzone exhaust duct diameter size or special work access opening window height these must be entered into the control system to assure the correct display values Press MENU to access these...

Страница 49: ...or altered Typically these default values are factory set based on the cabinet type model and size as previously discussed However they may be altered in special cases for modified cabinets The setpoi...

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Страница 51: ...r NU 565 600 Downflow setpoint 60 Downflow setpoint 60 Downflow high limit 66 Downflow high limit 66 Downflow low limit 54 Downflow low limit 54 Exhaust setpoint 740 Exhaust setpoint 1122 Exhaust high...

Страница 52: ...e necessary for proper cabinet performance The cabinet is considered to be certifiable if the following airflow measurements are present a Downflow average 60 LFPM 5 LFPM 30 m s 025 m s b Inflow avera...

Страница 53: ...ct inflow velocity Use Table 7 1 to relate downflow and exhaust inflow volumes and corresponding average airflow velocities It is always desirable to achieve airflow values that are set as close as po...

Страница 54: ...stated below Model Low Alarm Limit Pressure Switch Low Trip Point CFM CMH CFM CMH NU 565 400 629 1069 529 899 NU 565 600 954 1621 854 1451 If present remove cardboard from supply air intake and turn...

Страница 55: ...ption Set Up Step 1 Activate power to the cabinet Turn on exhaust system and make sure damper actuator is plugged into the 3 position connector on the top right side of the cabinet Access sensor setup...

Страница 56: ...city using the recommended procedure found in Table 7 0 If necessary adjust the exhaust system and or damper to achieve the correct inflow velocity Use Table 7 1 to relate downflow and exhaust inflow...

Страница 57: ...ft sides of the control box and rotate control box forward to access the pressure switch adjustment Pressure switch must be adjusted while control box is open Adjust as needed the adjustment dial unti...

Страница 58: ...edge of the window Spot check several points on the recommended downflow velocity Step 4 Press or button of supply duty cycle to adjust blower speed The objective of this spot check is to obtain the d...

Страница 59: ...t Setback Calibration Optional The night set back calibration is performed within the calibration mode after the inflow and downflow calibration procedure is complete NOTE If night setback exhaust air...

Страница 60: ...ent method The goal is to reduce the inflow volume to the following values which represents approximately 100 fpm 51m s inflow or may be set at any other values within the damper operational range of...

Страница 61: ...t filter is checked using a gross leak method since the exhaust filter is not easily scanned when connected to an exhaust system It may be leak tested by drilling a hole in the duct at a downstream lo...

Страница 62: ...low the wiper gasket The criteria used to evaluate the smoke patterns is the following 1 The smoke inside the cabinet shall show smooth downward flow with no dead spots or reflux 2 No smoke shall esca...

Страница 63: ...st using non hazardous materials A minimum of three 3 1 minute particle counts shall be sampled and recorded while the user simulates aseptic compounding manipulations At Rest Particle Count Test Take...

Страница 64: ...r should be turned on thus the inflow volume balanced with the downflow volume can be measured to set the desired average inflow velocity The inflow exhaust volume is measured by using a Direct Inflow...

Страница 65: ...3 8 1263 53 89 6 1369 58 05 4 1475 62 21 2 1580 66 37 5 1686 Number of Readings Average Velocity of Constricted Area ft min mps 1 Average Velocity of Constricted Area fpm m s 2 X Constricted Access Ar...

Страница 66: ...e from the table for the nominal average velocity the calibrated exhaust value entered should be 740cfm 1257CMH NOTE 2 For additional guidance the values provided below with a constant 60fpm 30mls dow...

Страница 67: ...AG FUSE SIZE 1 4 X 1 1 4 INCH 5 X 20MM 5 X 20MM NU 565 400 10 AMPS 3 AMPS 1 AMP NU 565 600 10 AMPS 3 AMPS 1 AMP 7 10 3 Main Control Board Replacement Note All setup and calibration data will be lost t...

Страница 68: ...rror in operation Cabinet type motor type and calibration data will not be affected with this reset Master Reset Resets all calibration cabinet type motor type sensor data and options settings back to...

Страница 69: ...OM0301 August 20 Page 69 of 105 Select desired function from menu Perform either reset function as selected below...

Страница 70: ...screen will revert back to the Nuaire logo main menu At this point the cabinet MUST be turned off to complete the process Either unplug the cabinet or use the power switch within the control center t...

Страница 71: ...ey matches push in and turn the locking ring clockwise until ring locks Proceed to calibrate sensor menu 7 11 2 Exhaust Pressure Sensor The exhaust pressure sensor is a digital differential velocity p...

Страница 72: ...arm will be on for 30 seconds upon initial alarm condition then once every ten seconds When presented with an error message please perform the following Step 1 NOTE ALL ERROR MESSAGES Error message wi...

Страница 73: ...verride is activated preventing the usage of the cabinet Power Loss Alert Indicates a power interruption has occurred Press DISPLAY to clear message Cabinet outlets won t turn on Check outlet fuse loc...

Страница 74: ...s Calibration Service parameter Entry into the Calibration Service functions requires a service password for entry After pressing the Calibration Service menu item a password screen will appear The de...

Страница 75: ...ust 20 Page 75 of 105 8 2 2 Calibration Service Menu The Calibration Service menu provides a list of sub menu items to accomplish all service tasks Each sub menu item will be described in the followin...

Страница 76: ...control is on the downflow and inflow displays will indicate nominal setpoints Airflow adjustments can be made in the manual mode by going into airflow calibration and adjusting the blower duty cycle...

Страница 77: ...arameter allows for the selection of time programmable from 30 to 240 seconds determining how often the exhaust controller updates the control signal to the automatic damper i e NU 951 012 if installe...

Страница 78: ...ing a constant exhaust alarm when the exhaust system is turned off Valid settings for the exhaust control damper position are open close freeze or off Off keeps the exhaust control alarms active when...

Страница 79: ...damper function will close during a remote override contact closure However this option allows the opposite damper functio 8 2 2 2 Service The service setup menu allows a QUALIFIED TECHNICIAN to conf...

Страница 80: ...eset both UV light and motor blower run timer Select desired run time parameter from menu UV Run Time Quantity of hours the UV light has logged ON Reset time for UV lamp replacement Motor Blower Run T...

Страница 81: ...feature to indicate an advance notice that re certification is due Press DUE to enter into the certification required time period screen Select desired interval of certification required i e No Remin...

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Страница 83: ...et the filter status data used to predict filter life availability Filter status is based on maximum RPM minus the starting RPM entered by technician then scaled to current RPM to determine filter per...

Страница 84: ...t conditions as shown Alarm Setup Alarm Silence This parameter allows for the selection of the alarm silence key function When the alarm silence function is on all current and future alarms will be si...

Страница 85: ...be silenced The time is displayed in minutes with a programmable range of 1 to 60 Downflow Alarm Time This parameter allows for the selection of time to determine how many continuous seconds of an ala...

Страница 86: ...OM0301 August 20 Page 86 of 105 Interlock Features All interlocks are enable disabled by a user through the display...

Страница 87: ...HLINK system UV window interlock there is a double redundant UV window interlock relay To override the UV window interlock for service purposes only both interlocks must be changed through the TOUCHLI...

Страница 88: ...switch is pending or blue the fan relay will be on or energized In either case the fan relay will be on when the blower switch is on or green and off when the blower switch is off or not colored Blowe...

Страница 89: ...his menu item provides the access to aux relay function and accessory outlet functions Aux Relay function This parameter allows for the selection of the AUX relay function When the AUX relay is on the...

Страница 90: ...has its own menu screen to excise the control system Select menu screen as desired Blower Sensor Diagnostics Test outputs allow a service technician to exercise these output functions Pressing TOGGLE...

Страница 91: ...tic Read inputs all a service technician to exercise or check these input functions The inputs may be checked by altering the state of the input function i e sliding window position and monitoring the...

Страница 92: ...utes and then remove This action will cause the cabinet to go into a downflow alarm condition The exhaust airflow reading should increase during this test However the downflow reading should go down b...

Страница 93: ...to aid the supply On Off and exhaust delay options The relay will activate whenever the blower is turned on and stay on unless after 5 minutes there is a low exhaust alarm then the relay will de acti...

Страница 94: ...that personnel leave the cabinet face area 10 1 2 Operation The operation of the ultraviolet light is accomplished by closing the sliding window and pressing the UV switch location on the front panel...

Страница 95: ...p hermolyticus alpha type 5 500 Staphylococcus aureus 6 600 Staphylococcus albus 5 720 Micrococcus sphaeroides 15 400 Sarcina lutea 26 400 Pseudomonas fluorescens 7 040 Escherichia coli 7 040 Proteus...

Страница 96: ...h laboratories and most industrial laboratories the expected transient over voltage is 2500 V for a 230 V supply and 1500 V for a 120 V supply 11 5 Pollution Degree 2 0 Pollution degree describes the...

Страница 97: ...Steel Worksurface Stainless Steel Window Faring Stainless Steel Window Glides HDPE Window Safety Glass Window Frame Stainless Steel Front Service Panel Painted Steel Front Decorative Panel Painted Ste...

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