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Chapter 1

Microscopy Procedures

29

Micr

oscopy

Pr
oced

ures

Epi-flu

o

re

scence

Mi
croscopy

Chapter 1-5

2

Close the shutter and block the illumination
path.

Set the shutter open/close lever of the epi-fluorescence
attachment to position “C” to close the shutter and block
the optical path.

Shutter of the epi-fluorescence attachment

The shutter blocks illumination.
If the specimen is continuously exposed to the strong
light of the mercury lamp, it may become damaged or
decolorized.
Be sure to close the shutter when suspending the
microscopy or when pausing epi-fluorescence
microscopy to perform microscopy with diascopic
light. Be sure to get into the habit of performing this
operation.

A

1 - 2 - 3

- 4

B

1 - 2 / 3

- 4

C

1 - 2 - 3 - 4

1

1

4

2

3

4

CUBE

Closing the shutter

Bringing the filter cube into the optical path

3

Bring the filter cube into the optical path.

Bring the desired cube into the optical path by turning the
filter cube switching knob.
(Confirm the address of the filter cube to be used, and
match the switching knob with the number.)

Selecting a filter cube

A filter cube consists of three types of optical
components: an excitation filter (EX filter), a
barrier filter (BA filter), and a dichroic mirror (DM).
Select the filter cube with the appropriate
combination of optical components for the
characteristics of the specimen and the
fluorescence dye.

4

Fully open the field diaphragm of the
epi-fluorescence attachment.

Push in the field diaphragm lever of the epi-fluorescence
attachment to open the diaphragm fully.

5

Turn on the mercury lamp.

See your illuminator's manual for details.

6

Open the shutter.

Set the shutter open/close lever of the epi-fluorescence
attachment to position “O” to open the shutter.

Light shielding plate of the

epi-fluorescence attachment

The light shielding plate protects the observer's
eyes from reflected ultraviolet light, which is
originally emitted from the objective at the
specimen.

A

1 - 2 - 3

- 4

B

1 - 2 / 3

- 4

C

1 - 2 - 3 - 4

1

1

4

2

3

4

CUBE

Fully opening the field diaphragm

Opening the shutter

Filter cube
switching knob

Filter cube
nameplate window

Field diaphragm

lever

Light shielding plate

Shutter closed (C)

position

Shutter opened
(O) position

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Содержание eclipse Ci-L

Страница 1: ...Upright Microscope Instruction Manual M568 E 11 8 NF 1 1 2 M568EN01 ...

Страница 2: ......

Страница 3: ...to ensure the accuracy of this manual errors or inconsistencies may remain If you note any points that are unclear or incorrect please contact your nearest Nikon representative Some of the equipment described in this manual may not be included in the set you have purchased If you intend to use any other equipment with this product read the manual for that equipment too If this equipment is used in...

Страница 4: ...e Microscopy Individual Operations Assembly Troubleshooting Maintenance and Storage Specifications and Safety Standards Bright field Microscopy Quick Guide Symbols Used in This Manual The following symbols are used in this manual Symbols for Safety WARNING CAUTION Highlights important information that should be noted for safety Read Safety Precautions for details Other Symbols Indicates informatio...

Страница 5: ...mbols Used in This Manual Safety Precautions Notes on Handling the Product Microscopy Procedures Bright field Microscopy Microscopy Procedures Phase Contrast Microscopy Microscopy Procedures Simple Polarizing Microscopy Microscopy Procedures Sensitive Tint Plate Microscopy Microscopy Procedures Epi fluorescence Microscopy Individual Operations Assembly Troubleshooting Maintenance and Storage Speci...

Страница 6: ...5 2 Epi fluorescence Microscopy Procedure 28 Individual Operations 33 1 Adjusting the Brightness of a Diascopic Image 33 1 1 Adjustment with the Dia illumination Brightness Control Knob 33 1 2 Adjustment with the ND Filter for Ci S 33 1 3 Removing an NCB filter for a brighter image Ci S 34 2 Focusing on the Specimen Vertical Stage Movement 35 2 1 Focus Knob Rotation and Stage Movement 36 2 2 Numbe...

Страница 7: ...Configuration 57 2 Assembly for Bright field Microscopy 58 3 Assembly for Phase Contrast Microscopy 62 4 Assembly for the Simple Polarizing Microscopy 62 5 Assembly for Sensitive Polarization Microscopy 63 6 Assembly for Epi fluorescence Microscopy 64 7 Attaching a Camera 66 Troubleshooting 67 1 Optical System and Operation 67 1 1 General 67 1 2 Epi fluorescence Microscopy 71 1 3 Phase Contrast Mi...

Страница 8: ...g of Symbols Used on the Product When appearing on this product the symbols below indicate the need for caution at all times during use Read the relevant instructions in this manual before attempting to use or adjust any part to which the symbol has been affixed Biohazard This symbol is affixed to the front of the stand of this product to call your attention to the following WARNING Using this pro...

Страница 9: ...ately after a period of illumination There is a risk of burn if you touch the hot area Always attach the lamphouse cover when using this product Make sure the lamp and surrounding areas have cooled sufficiently before attempting to replace the lamp about thirty minutes To avoid the risk of fire do not place fabric paper or highly flammable volatile materials such as gasoline petroleum benzine pain...

Страница 10: ...des Check to determine whether a sample is hazardous before handling If sample is hazardous handle it according to the standard procedure specified for your laboratory If the sample is potentially infectious wear rubber gloves and avoid directly touching samples If such a sample is spilled onto this product the portion must be decontaminated in a safety manner Consult your safety supervisor or saf...

Страница 11: ...cloth or something similar If water enters stop the use of the product and contact your nearest Nikon representative 5 Do not place any object on top of the product Do not place any object on top of this product 6 Cautions on assembling installing and carrying the product Take care to avoid pinching your fingers or hands during product assembly and installation Scratches or fouling optical compone...

Страница 12: ...this product may enter the objective as extraneous light If possible switch off the room lights directly above this product when making observations Select a location with minimal dust To avoid splashes do not use this product near water Make sure the ambient temperature is 0 to 40 C and humidity is 60 or less When transporting or storing this product the ambient temperature must be 20 to 60 C wit...

Страница 13: ...eceive d includin g interfer ence that may cause undesi red operati on This Class A digital appara tus complie s with Canadi an ICES 0 03 Cet appare il numéri que de la classe A est confirm e à la norme NMB 0 03 du Canada 940001 4N75 INSPE CTION EQUIP MENT 70 60 50 40 30 20 10 0 90 80 X knob Condenser focus knob Dia illumination brightness control knob Y knob Grip Condenser centering screw AC inle...

Страница 14: ... Adjust interpupillary 9 Focus and center condenser 10 Bring the desired objective into the optical path 11 Adjust the aperture diaphragm 12 Focus on specimen 13 Circumscribe field diaphragm to field of view 14 View specimen 15 Turn off the power CL AM P TO RQ UE ND4 ND8 OUT IN Preparation for microscopy 1 Turn on the power Press the switch to the position to turn on the power to the microscope th...

Страница 15: ...gm Turn the field diaphragm dial and the aperture diaphragm lever clockwise to open them completely CL AM P TO RQ UE ND4 ND8 OUT IN Fully open field and aperture diaphragms 4 Bring the 10x objective into the optical path Turn the nosepiece to bring the 10x objective into the optical path Turn the nosepiece until it clicks Bring the 10x objective into the optical path 5 Place a specimen on the stag...

Страница 16: ... ergonomic tube push in the optical path switching lever to distribute 100 light to the binocular section 2 Look into the eyepiece and turn the coarse focus knob away to raise the stage to the upper limit From there focus on the specimen by lowering the stage Switching the Optical Path 100 to the Binocular Part 3 When the focus was roughly adjusted using coarse focus knob turn the fine focus knob ...

Страница 17: ... adjustment reference position 2 Focus on the specimen using the 40x objective 3 Bring the 10x or 4x objective into the optical path Reference position for diopter adjustment 4 Look into the right eyepiece with your right eye and the left eyepiece with your left eye Turn the diopter adjustment ring of each eyepiece to focus on the specimen At this point no focus knobs are used 5 Repeat Steps 2 thr...

Страница 18: ...adjust the condenser centering screws to center the diaphragm image within the field of view 2 Bring the 40x objective into the optical path to check the focus and centering of the field diaphragm image Make adjustments in the same way as step 1 as necessary POWER 0 8 0 8 0 6 0 6 0 4 0 4 0 2 0 2 3 Turn the field diaphragm dial and adjust the field diaphragm image so that its size is almost the sam...

Страница 19: ...re diaphragm lever on the condenser to adjust the aperture diaphragm so that it is set to 70 to 80 of the numerical aperture of the objective used Be sure to adjust the aperture diaphragm each time you change the objective You can see the aperture diaphragm image with the centering telescope Right size of the aperture diaphragm 12 Focus on the specimen 1 Look into the eyepiece and adjust the brigh...

Страница 20: ...d reducing the image contrast In addition the specimen will become decolorized over a wider area Field diaphragm s adjustment timing Be sure to adjust the field diaphragm each time you change the objective Circumscribe around the field of view Adjusting the field diaphragm 14 View the specimen Rotate the stage knob to move the target If the target is not in focus use the focus knob to adjust the f...

Страница 21: ...hat may cause undesi red operati on This Class A digital appara tus complie s with Canadi an ICES 0 03 Cet appare il numéri que de la classe A est confirm e à la norme NMB 0 03 du Canada 940001 4N75 INSPE CTION EQUIP MENT 70 60 50 40 30 20 10 0 90 80 Condenser focus knob Dia illumination brightness control knob Grip Condenser centering screw AC inlet Input voltage label Eyepiece Objective Stage Ph...

Страница 22: ...ust diopter 9 Adjust interpupillary 10 Focus and center condenser 11 Bring the Ph annular diaphragm Ph1 position into the optical path 12 Center the Ph annular diaphragm 13 Bring the desired Ph objective into the optical path 14 Match the Ph codes of annular diaphragm and the objective 15 Focus on specimen 16 Circumscribe field diaphragm to field of view 17 View specimen 18 Turn off the power CL A...

Страница 23: ...re diaphragm from the optical path CL AM P TO RQ UE P H A S E C O N T R A S T 0 9 0 D R Y J A P A N A Move the condenser turret to the A empty position 5 Bring the 10x Ph objective Ph1 into the optical path 6 Place a specimen on the stage and move the stage to bring the target into view 7 Focus on the specimen See Chapter 2 2 Focusing on the Specimen for details 8 Adjust the diopter See Chapter 2 ...

Страница 24: ... been placed into the optical path and that the Ph1 symbol on the condenser turret is facing the front 2 Rotate the stage knob to move the specimen and bring a portion where there is no sample under the cover glass into the optical path 3 Remove one eyepiece from the tube and insert the centering telescope with adapter into the tube 4 Hold the flange of the centering telescope and rotate the eyepi...

Страница 25: ...ed on the Ph objective depending on the size of the phase plate Ph codes have nothing to do with the magnification of the objective Always use a Ph objective and Ph annular diaphragm with the same Ph code You cannot experience the phase effect if a different combination of the codes might be used Centering of the annular diaphragm and the phase plate The position of each annular diaphragm in the c...

Страница 26: ...sure to adjust the field diaphragm each time you change the objective Circumscribe around the field of view Adjusting the field diaphragm 17 View the specimen Rotate the stage knob to move the target If the target is not in focus use the focus knob to adjust the focus To switch to bright field microscopy Turn the condenser turret until the A empty symbol comes to the front Microscopy is possible w...

Страница 27: ...interfer ence that may cause undesir ed operati on This Class A digital appara tus complie s with Canadi an ICES 0 03 Cet appare il numériq ue de la classe A est confirm e à la norme NMB 00 3 du Canada 940001 4N75 INSPEC TION EQUIP MENT 70 60 50 40 30 20 10 0 90 80 Condenser focus knob Dia illumination brightness control knob Grip Condenser centering screw AC inlet Input voltage label Eyepiece Obj...

Страница 28: ...s on specimen 8 Adjust diopter 9 Adjust interpupillary 10 Focus and center condenser 11 Bring the portion without sample into the optical path 12 Install polarizer unit 13 Adjust orientation of analyzer and polarizer 14 Bring the desired objective into the optical path 15 Focus on specimen 16 Circumscribe field diaphragm to field of view 17 View specimen 18 Turn off the power CL AM P TO RQ UE ND4 ...

Страница 29: ...e with simple analyzer to remove the analyzer from the optical path The polarizer unit for simple polarization has not yet been installed at this point Remove the analyzer from the optical path 7 Focus on the specimen See Chapter 2 2 Focusing on the Specimen Vertical Stage Movement for details 8 Adjust the diopter See Chapter 2 4 Adjusting the Diopter for details 9 Adjust the interpupillary distan...

Страница 30: ...ion mark on the polarizer roughly comes to the front at this point Keep the fixing screw of the polarizer unit loosened CL AM P TO RQ UE ND4 ND8 OUT IN ND4 ND8 OUT IN Bringing the analyzer into the optical path Installing the polarizer Slider type analyzer for simple polarization Using the D SA Analyzer Slider for Simple Polarization instead of an analyzer tube for simple polarization can keep the...

Страница 31: ...may result in uneven illumination around the field of view 15 Focus on the specimen 1 Look into the eyepiece and adjust the brightness of the field of view by turning the dia illumination brightness control knob You can also adjust the brightness with an ND filter for Ci S 2 Rotate the stage knob to move the stage and bring the target into the optical path 3 If the specimen is not in focus turn th...

Страница 32: ...arizing microscopy If you need a retardation measurement or stricter polarizing observation use a dedicated polarizing microscope To switch to bright field microscopy Pull out the analyzer IN OUT knob to remove the analyzer from the optical path Remove the polarizer unit from the field lens 18 Turn off the power Turn off the power switch press to the O position for the microscope The power LED on ...

Страница 33: ... interfer ence that may cause undesir ed operati on This Class A digital appara tus complie s with Canadi an ICES 0 03 Cet appare il numériq ue de la classe A est confirm e à la norme NMB 00 3 du Canada 940001 4N75 INSPEC TION EQUIP MENT 70 60 50 40 30 20 10 0 90 80 Condenser focus knob Dia illumination brightness control knob Grip Condenser centering screw AC inlet Input voltage label Eyepiece Ob...

Страница 34: ...ocus on specimen 8 Adjust diopter 9 Adjust interpupillary 10 Focus and center condenser 11 Bring the portion without sample into the optical path 12 Install polarizer unit 13 Adjust orientation of analyzer and polarizer 14 Bring the desired objective into the optical path 15 Focus on specimen 16 Circumscribe field diaphragm to field of view 17 View specimen 18 Turn off the power CL AM P TO RQ UE N...

Страница 35: ...late analyzer unit to remove the analyzer from the optical path The polarizer unit for first order red compensation has not been installed at this point Removing the analyzer from the optical path 7 Focus on the specimen See Chapter 2 2 Focusing on the Specimen Vertical Stage Movement for details 8 Adjust the diopter See Chapter 2 4 Adjusting the Diopter for details 9 Adjust the interpupillary dis...

Страница 36: ...rizer roughly comes to the front at this point Keep the fixing screw of the polarizer unit loosened CL AM P TO RQ UE ND4 ND8 OUT IN ND4 ND8 OUT IN Bringing the analyzer into the optical path Installing the polarizer Slider type analyzer for first order red compensation Using the C AS Analyzer Slider for First order Red Compensation instead of an analyzer tube for first order red compensation can k...

Страница 37: ... objective into the optical path Turn the nosepiece to bring the desired objective into the optical path Swing out condenser 1 100x Mounting a 1 100x swing out condenser on the Ci L main body when using the 1x objective may result in uneven illumination around the field of view 15 Focus on the specimen 1 Look into the eyepiece and adjust the brightness of the field of view by turning the dia illum...

Страница 38: ...al Leftmost Rightmost Urate crystal Calcium pyrophosphoric acid crystal Keep the lambda plate clean Note that dirt such as dust and fingerprint on the lambda plate can significantly degrade the polarization performance Keep it clean To switch to bright field microscopy Pull out the analyzer IN OUT knob to remove the analyzer from the optical path Remove the polarizer unit from the field lens 18 Tu...

Страница 39: ...u s complies with Canadia n ICES 00 3 Cet appareil numériq ue de la classe A est confirme à la norme NMB 00 3 du Canada 940001 4N75 INSPEC TION EQUIPM ENT 70 60 50 40 30 20 10 0 90 80 X knob Condenser focus knob Dia illumination brightness control knob Y knob Grip Condenser centering screw AC inlet Input voltage label Eyepiece Objective Stage Tube Nosepiece Main unit The figure shows ECLIPSE Ci S ...

Страница 40: ...ecimen 1 Turn off the dia illumination power 2 Close the shutter 3 Bring the filter cube into the optical path 4 Fully open the field diaphragm 5 Turn on the mercury lamp 6 Open the shutter 7 Bring the desired objective into the optical path 8 Focus on specimen 9 Circumscribe field diaphragm to field of view 10 View specimen 11 Turn off the mercury lamp CL AM P TO RQ UE ND4 ND8 OUT IN INTENSILIGHT...

Страница 41: ...ed and match the switching knob with the number Selecting a filter cube A filter cube consists of three types of optical components an excitation filter EX filter a barrier filter BA filter and a dichroic mirror DM Select the filter cube with the appropriate combination of optical components for the characteristics of the specimen and the fluorescence dye 4 Fully open the field diaphragm of the ep...

Страница 42: ...n the focus knob to focus on it ND4 ND8 ND16 A 1 2 3 4 B 1 2 3 4 C 1 2 3 4 Adjusting the brightness with ND filters 9 Adjust the field diaphragm of the epi fluorescence attachment Use the field diaphragm lever of the epi fluorescence attachment to adjust the field diaphragm so that it almost circumscribes the field of view Size of the field diaphragm Normally adjust the field diaphragm so that it ...

Страница 43: ...uring fluorescence observations To return to bright field microscopy Close the shutter of the epi fluorescence attachment and block the light emitted by the mercury lamp Turn on the microscope power switch to turn on the dia illumination lamp Turn the filter cube switching knob and bring the position where a filter cube is not located into the optical path 11 Turn off the mercury lamp See your ill...

Страница 44: ...Chapter 1 Microscopy Procedures 32 Chapter 1 5 Microscopy Procedures Epi fluorescence Microscopy ...

Страница 45: ...htness control knob to the position and use the ND filters for brightness adjustments 1 2 Adjustment with the ND Filter for Ci S ND filters are used to adjust light intensity Higher filter numbers correspond to lower transmittance i e darker images The color balance of the image will not change Ci S has built in ND filters ND4 and ND8 Pushing the each filter IN OUT switch inserts the corresponding...

Страница 46: ... cord from the receptacle 2 Wait until the lamp and the peripheral devices cool down sufficiently about thirty minutes 3 Remove all accessories such as the camera objective nosepiece eyepiece tube condenser and the stage See Chapter 3 Assembly and use a reverse order of assembling to remove the accessories 4 Place the microscope main body upside down with its bottom facing up 5 Loosen two NCB filt...

Страница 47: ...er limit 4 Look into the eyepiece and turn the coarse focus knob slowly to lower the stage and focus on the specimen Release your hand from the coarse focus knob once it has been focused 5 Turn the fine focus knob to adjust the focus more accurately TO RQ UE CL AM P POWER 0 90 80 70 60 50 40 30 20 10 Using the coarse focus knob to focus Using the fine focus knob for more accurate focus Raising the...

Страница 48: ...the rear Stage is raised TO RQ UE CL AM P POWER 0 90 80 70 60 50 40 30 20 10 Stage Vertical Movement 2 2 Number of Focus Knob Turns and Distance of Stage Travel Number of Focus Knob Turns and Distance of Stage Travel No of knob turns Distance of stage travel vertical direction One rotation of the coarse focus knob Approx 9 33 mm One rotation of the fine focus knob Approx 0 1 mm One scale of the fi...

Страница 49: ... the fine focus knob will not be locked Using this function you can refocus with ease by simply turning the coarse focus knob to the limit This is helpful when switching between similar specimens during the observation 1 With the focus set on the specimen tighten the coarse focus clamp ring by turning it approximately 3 4 of a rotation in the direction of the arrow on the base of the microscope Th...

Страница 50: ...d to the coarse focus knobs using magnet so you can detach the left and right knobs from the coarse focus knobs and swap them Position them to best suit your usage Removing a flat focus knob A flat fine focus knob can be easily removed by inserting a flathead screwdriver or the likes into the notch in the knob POWER 0 8 0 6 0 4 0 2 Position Exchange of the Fine Focus Knob Flat fine focus knob Conv...

Страница 51: ...ge Movement 3 2 Adjusting the Knob Heights The heights positions of the X knob and Y knob can be changed Hold the knob and move it along the vertical axis to the desired height 3 3 Adjusting the Knob Rotation Torque When the X knob and Y knob are moved to the top and bottom positions the torque adjustment screws can be found between the knobs Turning the torque adjustment screw to move it closer t...

Страница 52: ...ion 2 Follow Steps 1 through 6 in Chapter 1 1 2 Bright field Microscopy to focus on the specimen using the 10x objective 3 Turn the nosepiece to bring the 40x objective into the optical path and turn the coarse focus knob and then the fine focus knob to focus on the specimen 4 Bring the 10x or 4x objective into the optical path 5 Look into the left eyepiece with your left eye Without touching the ...

Страница 53: ... the field diaphragm image size is almost the same as the field of view 7 When the center of the field diaphragm image is not centered turn the condenser centering screws to move the field diaphragm image to the center of the field of view This is easiest if you adjust the field diaphragm aperture so that it is slightly smaller than the eyepiece field of view Correct focusing of the field diaphrag...

Страница 54: ...ot recommend setting the aperture diaphragm to less than 60 of the numerical aperture of the objective Adjustment timing for the aperture diaphragm Be sure to adjust the aperture diaphragm each time you change the objective 6 1 Adjusting the Aperture Diaphragm Using the Condenser Scale The scale on the condenser indicates the numerical aperture The index on the aperture diaphragm lever should be a...

Страница 55: ... achieved For example when an objective with a numerical aperture of 1 4 is used the maximum aperture of the swing out condenser or the Abbe condenser will only be about 65 of the N A of the objective even when the condenser s aperture diaphragm is wide open Refer to Section 14 Tips for the Phase Contrast Microscopy for the phase contrast condenser 8 Adjusting the field diaphragm The field diaphra...

Страница 56: ... 0 C TE2 Ergonomic tube Pulled out 50 50 Pushed in 100 0 Pulled out by one notch 20 80 T C TT trinocular tube Pulled out by two notches 0 100 Pushed in 100 0 F C TF trinocular tube Pulled out 0 100 9 2 Disabling the Clicking of the Optical Path Switching C TT trinocular tube and F C TF trinocular tube have a NO CLICK switch on their tube attaching surface Slide this switch in the direction of the ...

Страница 57: ...n the tube and the microscope arm allows you to raise the eye point 25 mm higher Using the eyelevel riser 11 Using Stage at Lowered Position Spacer for Nosepiece Inserting a spacer for the nosepiece between the arm and the nosepiece allows for operation with the stage set 20 mm lower A lowered stage facilitates specimen replacement during a cytodiagnostic examination etc Using the filter cassette ...

Страница 58: ... will degrade image quality Be careful to prevent bubbles from forming To check for air bubbles fully open the field diaphragm and aperture diaphragm remove the eyepiece and examine the exit pupil bright round section of the objective inside the eyepiece tube If it is difficult to ascertain the presence of bubbles attach a centering telescope with the adapter then look for air bubbles while turnin...

Страница 59: ...e adjusted These lenses also are further subdivided into several types depending on the properties of the internal phase plate For favorable microscopy results the phase contrast amount of the specimen must match the properties of the phase plate See the table below for the use properties of the Ph objectives When using a dark contrast Ph objective make sure that the phase contrast of the specimen...

Страница 60: ...ic at each magnification In addition slightly decentering the annular diaphragm and the phase plate will produce a shadowing effect resulting in a stereo image Use this method as appropriate for the specimen Infrared ray blocking filter Ci S only The lamphouse is equipped with an infrared ray cut filter which reduces the infrared ray component of the illumination to protect live specimens from bei...

Страница 61: ...bjective into the optical path Fully open the aperture diaphragm To use epi fluorescence and phase contrast microscopy concurrently 1 Use phase contrast microscopy to adjust the focus onto the target 2 Remove the GIF filter if installed on the field lens 3 Insert the desired excitation filter cube into the optical path 4 Open the shutter for the epi fluorescence attachment to focus it again 5 Adju...

Страница 62: ...rsion oil For fluorescence observations be sure to use a non fluorescent slide cover glass and our designated immersion oil for an image with better contrast Restricting the illumination to the area of the specimen being viewed adjusting the field diaphragm The field diaphragm is used to restrict illumination to the area of the specimen being viewed Turning the field diaphragm lever of the epi flu...

Страница 63: ...rocedure for epi fluorescence microscopy is to first locate the target under differential interference contrast or phase contrast microscopy and then switch to epi fluorescence microscopy To locate the target under dia illumination bright field microscopy you will need to note the following Under dia illumination bright field microscopy start with a 10x objective and adequately stop down the conde...

Страница 64: ...e types of filter cubes cannot be inserted directly into the epi fluorescence attachment Follow the procedure described in Chapter 3 Section 6 Attaching a filter cube to remove an internal spacer or reverse the spacer before insertion Excitation filter EX filter Excitation filters allow selective transmission of light excitation light in the wavelength range required for fluorescent light emission...

Страница 65: ...inct tending to darken the background of fluorescent images However recent developments in filter performance have resulted in increased use of filters of short cut on wavelengths 2 For multiple labeled specimens use an LP filter for microscopy of fluorescent images of all fluorophores Note that a combination involving an ordinary dichroic mirror an excitation filter and an LP filter type barrier ...

Страница 66: ... on the monitor should be in the opposite direction of the stage When the stage is moved in the direction from left to right the image on the monitor should move from right to left After making the appropriate adjustments tighten the screws firmly Adjusting the camera head attachment position 3 Focus the image If the image viewed through the eyepiece appears to be in focus but the image on the mon...

Страница 67: ...usting the condenser Always focus and center the condenser For phase contrast microscopy center the annular diaphragm The diaphragm aperture should generally be adjusted to 70 to 80 of the numerical aperture of the objective Confirming the photomicrographic range The image on the monitor represents the photomicrographic range Confirming the focus Check the focus both through the eyepiece and on th...

Страница 68: ...d light from the illuminator reduces the contrast of an image insert an optional IR cut filter in the field lens before attaching the polarizer unit Cover the IR cut filter for the simple polarizer over the field lens Screw the IR cut filter for a sensitive tint plate polarizer into the bottom of the polarizer unit for first order red compensation ND4 ND8 OUT IN ND4 ND8 OUT IN ND4 ND8 OUT IN ND4 N...

Страница 69: ...ber illuminator Ergonomic tube Binocular tube Trinocular tube DSC port Centering telescope CFI eyepiece TV adapter Ci S Ci L main body Spacer for the nosepiece Sextuple nosepiece with analyzer slot Sextuple nosepiece Analyzer slider Stage CFI objective Condenser Filter cassette holder Polarizer unit ENG mount camera C mount camera photomicrography device DS U3 L3 DS camera control unit Camera head...

Страница 70: ... operation This Class A digital apparatus complies with Canadian ICES 003 Cet appareil numériqu e de la classe A est confirme à la norme NMB 003 du Canada 940001 4N75 INSPECT ION EQUIPME NT 70 60 50 40 30 20 10 0 90 80 Checking the input voltage 2 Attaching the stage 1 Turn the coarse focus knob to remove the cushioning material from the substage section 2 Turn the coarse focus knob until the elev...

Страница 71: ...UE CL AM P 0 90 80 70 60 50 40 30 20 10 Attaching the condenser 4 Attaching the tube Place the tube on the microscope arm and secure it by tightening the clamp screw on the arm Attaching the tube Using the eyelevel riser Place the eyelevel riser on the microscope arm and secure it by tightening the clamp screw on the arm Attach the tube on the eyelevel riser and secure it by tightening the clamp s...

Страница 72: ...e spacer for the nosepiece 1 Slide the spacer for the nosepiece along the groove to attach it to the nosepiece and secure it in place with the provided tool 2 Lower the stage to the limit 3 Place the nosepiece with the spacer on the microscope following the procedure described in Attaching the nosepiece on the previous page Attaching the spacer for the nosepiece Exclusivity of the spacer for the n...

Страница 73: ...rformance Properties for the specified power cords To prevent electric shock always turn off the power switch press to the O position for the microscope before connecting or disconnecting the power cord 1 Check that the microscope power switch is OFF 2 Plug the power cord into the AC inlet at the back of the microscope 3 Plug the other end of the power cord into a wall outlet Assembly of the syste...

Страница 74: ...the tube on the analyzer unit and tighten the tube clamp screws of the analyzer unit using the tool provided with the microscope to secure the tube Slider type analyzer for simple polarization When using the D SA Analyzer Slider for Simple Polarization instead of an analyzer tube for simple polarization insert it into the analyzer slot of the nosepiece To use the D SA Analyzer Slider for Simple Po...

Страница 75: ...Compensation instead of an analyzer tube for first order red compensation insert it into the analyzer slot of the nosepiece To use the C AS Analyzer Slider for First order Red Compensation the C NA sextuple nosepiece with analyzer slot is required CL AM P TO RQ UE ND4 ND8 OUT IN ND4 ND8 OUT IN Attaching an analyzer tube for first order red compensation polarizer unit for first order red compensati...

Страница 76: ...hment and tighten the tube clamp screws of the epi fluorescence attachment using the tool provided with the microscope to secure the tube A 1 2 3 4 B 1 2 3 4 C 1 2 3 4 1 1 4 2 3 4 CUBE Attaching the epi fluorescence attachment Attaching a filter cube WARNING Always turn off the mercury lamp before inserting or removing a filter cube 1 Remove the filter cube replacement cover on the left of the epi...

Страница 77: ...d barrier filters The excitation filter barrier filter and dichroic mirror can be removed from the filter cube for replacement Excitation filters are screw in filters while barrier filters are slide in filters Align the projection on the barrier filter with the groove on the filter cube and turn clockwise by approximately 30 degrees to secure it in place Replacing the excitation and barrier filter...

Страница 78: ...ith a camera cable 5 Use a camera trigger cable to connect the DSC connector on the back of the microscope to DS U3 or DS L3 Notes when connecting cables When connecting a capture cable to the DSC connector insert it to the end Attaching a camera head Prior to photomicrography adjust the camera position as appropriate See Chapter 2 16 1 Photomicroscopy Installing a camera head to the trinocular tu...

Страница 79: ...lean the objective Chapter 5 2 1 Lens Cleaning 4 Field diaphragm image is not focused on the specimen surface Condenser is not correctly adjusted 4 Make sure the condenser is focused and centered Chapter 2 5 Focusing and Centering the Condenser Dirty or dusty field of view when looking into eyepiece 5 An aperture diaphragm is stopped down too far 5 Open it to proper size Chapter 2 6 Adjusting the ...

Страница 80: ...The lamp voltage is too high Turn the brightness control knob to the mark position and adjust the brightness with ND filters Chapter 2 1 Adjusting the Brightness of a Diascopic Image Lamp voltage is too low Turn the brightness control knob to the mark position and adjust the brightness with ND filters Chapter 2 1 Adjusting the Brightness of a Diascopic Image Condenser aperture diaphragm is stopped...

Страница 81: ...1 Replacing the Lamp for Ci S Lack of visibility around periphery of field of view Illumination is uneven across the field of view Field of view is not visible Lens and specimen are dirty or dusty Clean them as appropriate Chapter 5 2 1 Lense Cleaning The specimen is upside down Turn up the cover glass and attach it to the stage Chapter 2 2 1 Bright field Microscopy Procedure 5 Place a specimen on...

Страница 82: ... Microscopy 3 Attaching the condenser Interpupillary adjustment has not been performed Perform interpupillary adjustment Chapter 2 2 1 Bright Field Microscopy Procedure 8 Adjust the interpupillary distance Images in left and right eyepieces are not coincident Diopter adjustment has not been performed Perform diopter adjustment See Chapter 2 4 Adjusting the Diopter in the Operation Diopter adjustme...

Страница 83: ... a mercury lamp Chapter 2 15 Tips for Epi fluorescence Microscopy Protecting the specimen and preventing it from decoloration shutter for the epi fluorescence attachment Mercury lamp on the HG precentered fiber illuminator has reached the end of its product life Replace the lamp Check your illuminator s manual A designated objective is not used at UV or V excitation Use a designated objective The ...

Страница 84: ...tch Put the Ph annular diaphragm with the same Ph code as the objective into the optical path Chapter 1 2 2 Phase Contrast Microscopy Procedure 14 Adjust the Ph annular diaphragm in the condenser with the Ph objective to be used The phase contrast of the specimen is too large Change the mounting agent or thickness of the specimen when preparing the specimen Chapter 2 14 Tips for Phase Contrast Mic...

Страница 85: ...amp for Ci S Capture button Problem Cause Measure The capture button does not function Camera trigger cable is not properly connected Attach it correctly Chapter 3 7 Attaching a Camera 2 2 Epi fluorescence Microscopy Problem Cause Measure There is no power supplied Plug in the power cord Check your illuminator s manual Lamp has burned out Replace the lamp with the specified type Check your illumin...

Страница 86: ...Chapter 4 Troubleshooting 74 Chapter 4 Troubleshooting ...

Страница 87: ...f the lamp Lamphouse cover Make sure the lamphouse cover is securely fitted to the lamphouse after lamp replacement Used lamps Do not break used lamps It should be disposed of as industrial waste according to local regulations and rules 1 Remove the lamphouse cover on the back of the microscope Remove the old lamp 2 Attach a new lamp Avoid touching the glass surface of the lamp with your bare hand...

Страница 88: ... used only to wipe off immersion oil from the objective and never to clean the entrance lens at the bottom of the eyepiece tube prism surface of the eyepiece tube or the filters 2 1 Cleaning Lenses Keep the lens free of dust fingerprints etc If there is contamination on the lenses or filters image quality decreases If any of the lenses become dirty clean them by following the procedure given below...

Страница 89: ...sal If contact occurs between a sample and this product determine whether the sample is hazardous If the sample is hazardous follow the standard procedures for your facility 3 Storage Store this product in a dry location where mold is unlikely to form Storage conditions are as follows temperature 20 C to 60 C humidity 90 RH max no condensation Store the objectives and eyepieces in a desiccator or ...

Страница 90: ...Chapter 5 Maintenance and Storage 78 Chapter 5 Maintenance and Storage ...

Страница 91: ...ing them Intended user It is intended for the medical professional and those who work on experimentations in the field of pathology and cytology 2 Performance Properties Nikon Microscope ECLIPSE Ci S Model ECLIPSE Ci S Optical system Infinity corrected CF optical system Objective CFI60 Eyepiece Field number 22 with ergonomic tube binocular tube 25 with T F trinocular tube Nosepiece Sextuple Focus ...

Страница 92: ...hable power cord set 3 conductor grounding 3 conductor grounding Type SVT No 18 AWG 3 m long maximum rated at 125 VAC minimum When used in 220 240 V regions Detachable power cord set approved in accordance with EU EN standard 3 conductor grounding 3 conductor grounding Type H05VV F 3 m long maximum rated at 250 VAC minimum When used inside Japan PSE approved detachable power cord set 3 conductor g...

Страница 93: ...i L Operating conditions Temperature 0 C to 40 C Humidity 60 RH max no condensation Altitude 2000 m max Pollution degree Degree 2 Installation Category II Electrical shock protection class Class I Indoor use only Transport storage conditions Temperature 20 C to 60 C Humidity 90 RH max no condensation External dimensions and weight Main body only External dimensions 223 W x 331 5 H x 331 D mm exclu...

Страница 94: ...Chapter 6 Specifications and Safety Standards 82 Chapter 6 Specifications and Safety Standards ...

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