NEW BRUNSWICK SCIENTIFIC BioFlo 4500, Руководство по эксплуатации

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New Brunswick Scientific Co., Inc. User’s Guide
59
4. Ensure sure that the stainless steel port steam cap is in place.
5. Open the
STEAM INLET VALVE
. Allow steam to pass through the
valve for about 15 minutes.
6. Close the
STEAM INLET VALVE
. Allow the valve to cool thoroughly
before using.
7. Reduce the vessel pressure to 0 PSIG using the
BACK PRESSURE
REGULATOR
located in the upper left rear corner of the open piping
frame (manual pressure control) or set the setpoint to “O” in the
PRESSURE
DISPLAY GAUGE
on the touchscreen
.
8. Open the valve on the sterile quick-connect.
9. To open the drain, rotate the drain valve handle as far as it will allow
so it is facing the front of the vessel. When the valve is open, the word
OPEN
is visible on the end of the handle.
10. Draining of the liquid may be accomplished by gravity, a peristaltic
pump or by using the vessel pressure to drive out the liquid from
inside the vessel.
11. After draining the vessel, close the drain valve by rotating the drain
valve 180
°
.
12. In the closed position, the handle should be facing the front panel
console and the word
CLOSE
is visible on the end the handle. Open
the valve on the sterile quick-connect.
13. Close the valve on the quick-connect fitting. Detach the sterile quick-
connect from the harvest/drain port.
14. Put the stainless steel port steam cap back on the port. Re-sterilize the
port.