NEW BRUNSWICK SCIENTIFIC BioFlo 4500, Руководство по эксплуатации

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New Brunswick Scientific Co., Inc. User’s Guide
167
Dry components may be added through this port. Liquid may then be added; the
solids dissolve in the vessel (the unit and agitator may be turned on to facilitate
dissolution of the solids). Do not add liquid beyond the maximum working
volume allowable for the vessel, and remember to allow room for the inoculum
which will be added after sterilization.
Liquid media may also be added through the charging/ light port or through any
of the other ports normally plugged in the headplate.
Once the media has been added, the port used must be closed.
Also verify that all valves are closed.
NOTE:
Media, liquid and solid, can be also added to the vessel by removing
the headplate and adding the required components. If media is
added in this manner, make sure that the headplate is reinstalled
securely in place prior to turning on the system.
27.3 Sterilization of Vessel Using Heat-Labile Media
Cell culture media is often composed of a variety of heat-labile components. If
filter-sterilized media is to be added to the vessel, the vessel may be sterilized
empty or with phosphate-buffered saline (PBS) in the vessel. After sterilization
the PBS can be aseptically removed and pre-sterilized liquid media (or media
components) can be added aseptically though a re-sterilizable addition port.
If the vessel is to be sterilized empty, the instructions for sterilization with liquid
in the vessel should be followed
with the exception that the drain valve should
be open instead of closed during sterilization and a slight modification of the
sterilization valve sequence be performed
. (Refer to the Section 28, Automatic
Sterilization.)